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South African Journal of Enology and Viticulture

On-line version ISSN 2224-7904
Print version ISSN 0253-939X

S. Afr. J. Enol. Vitic. vol.40 n.2 Stellenbosch  2019

http://dx.doi.org/10.21548/42-2-3342 

ARTICLES
doi:https://doi.org/10.21548/42-2-3342

 

Recent Developments in Seedless Grapevine Breeding

 

 

M. Akkurt*; H. Tahmaz; S. Veziroğlu

Department of Horticulture, Faculty of Agriculture, Ankara University, Ankara, Turkey

 

 


ABSTRACT

Grapes are one of the most commonly produced fruit crops in the world and are consumed as table grapes, wine grapes and dried grapes. In line with consumer demands, seedlessness continues to be an important feature that increases the chances of marketing grapes. Nearly all of the dried grapes that are offered to the world markets are seedless grapes. In addition, the demand for seedless varieties has increased steadily in the table grape trade over the years. Therefore, the production of new seedless grape varieties with large berries that are suitable for table consumption is one of the important goals of breeding studies in viticulture. In this study, recently bred and released new seedless grape varieties are briefly introduced, seedless grape breeding methods are summarised, and the latest developments in breeding methods are explained.

Keywords: Grape, seedlessness, breeding, variety


 

 

INTRODUCTION

Consumer demand for seedless grape varieties - both dried and table grapes - has been increasing worldwide. Almost all of the dried grapes consumed in the world markets consist of seedless grape varieties. In principle, seedlessness is produced in two mechanisms: through parthenocarpy and through stenospermocarpy. Seedless fruit set occurs without fertilisation and pollination in parthenocarpic fruit set (Perl et al., 2000). Parthenocarpic grape varieties are not preferred in the market due to their small berry size, while stenospermocarpic varieties experience a greater demand due to both table fruit and dried fruit quality. In stenospermocarpic fruit set, fertilisation and pollination occur normally as in the case of seeded grape varieties. However, with genetically controlled mechanisms, the development of integuments in the ovules after pollination is prevented. As a result of degeneration of the embryo and then the endosperm, seed development stops, with only partially formed seeds or seed traces being left (Costantini et al., 2008).

 

INHERITANCE OF SEEDLESSNESS

Different hypotheses have been proposed to determine the inheritance of seedlessness in grapevines. While some researchers have stated that seedlessness is controlled by recessive genes, another group of researchers has reported that it is controlled by different numbers of dominant genes (Constantinescu et al., 1972, Spiegel-Roy et al., 1990, Sato et al., 1994, Ledbetter & Burgos, 1994). In recent years, the most accepted model for the inheritance of seedlessness is that seedlessness is controlled by three independent recessive genes that are regulated by a dominant gene named SdI (seed development inhibitor) (Bouquet & Danglot, 1996, Lahogue et al., 1998, Adam-Blondon et al., 2001, Doligez et al., 2002).

 

SEEDLESS GRAPE-BREEDING METHODS

The breeding of seedless, colourful, disease-resistant new grape varieties with large berries is one of the important topics studied in viticulture. In addition to traditional methods such as clonal selection and conventional crossbreeding, biotechnological methods such as embryo rescue techniques and polyploidy have gained importance in seedless grape breeding. Furthermore, supportive genetic techniques, such as early selection with molecular markers in conventional crossbreeding, have also started to be used widely in seedless grape breeding (Degirmenci & Marasali Kunter, 2007).

Conventional crossbreeding

In conventional crossbreeding studies on breeding seedless grape varieties, a seedless parent is used as the father (pollinator) and a seeded parent is used as the mother. However, in seeded x seedless crosses, the seedlessness rate obtained in the progeny is , varying between 0% and 49%, depending on the parental combination (Spiegel-Roy et al., 1990). The successful culture of the abortive embryos of stenospermocarpic grapevine varieties has enabled seedless x seedless crosses in conventional crossbreeding studies. This practice (embryo rescue technique) has increased the rate of seedlessness observed in F1 plants in seedless x seedless crosses, and the seedlessness rate in the progeny has ranged from 16.7% to 92%, depending on the parental combination (Ramming et al., 1990). During the past 30 years, embryo rescue has been used mostly in seedless grapevine breeding programmes in the United States, Israel, South Africa, Chile and Australia. "Prime", "Mystery", "Spring Blush" and "Black-finger" are seedless table grape cultivars developed in Israel (Perl et al, 2003). Similarly, many studies have been published in the literature reporting the successful use of the embryo rescue technique in crossbreeding studies in viticulture (Ramming et al., 2000; Yang et al., 2007; Tian & Wang, 2008; Ji & Wang, 2013; Uquillas et al., 2013; Li et al, 2014, 2015).

Many seedless grape varieties obtained by conventional crossbreeding studies have been offered to the world grape market. The names of some of these table grape varieties and parental breeds are as follows: Autumn Royal (Autumn Black x Fresno C74-1; black), Beauty Seedless (Koenigin der Weingaerten x Black Kishmish; black), Crimson Seedless (Emperor x Fresno C33-199; Red-black), Fantasy Seedless (Fresno B 36-27 x Fresno C 78-68; black), Flame Seedless ((Cardinal x Sultanina) X (Red Malaga x Tifafihi Ahmer) X (Muscat of Alexandria x Sultanina); red), Ruby Seedless (Emperor x Sultana Moscata; red), Scarlet Royal (C33-30 x C103-41; red), Sunred Seedless (Datal x Ruby Seedless; red), Sweet Scarlet (C33-30 x C51-63; red), Superior Seedless (Cardinal x ? (seedless variety), white) (Julius Kühn Institute, 2018a).

Marker-assisted selection in crossbreeding

Since breeding by the conventional crossbreeding method requires a long time and high costs, studies on the development of selection with the help of DNA-based markers have gained importance in recent years. In this context, the segregation of seedless genotypes in the progeny obtained by the crossbreeding of seeded and seedless parents is performed with the help of molecular markers. Based on the hypothesis proposed by Bouquet and Danglot (1996), Lahogue et al. (1998) developed a codominant SCAR (sequence characterised amplified region) marker called SCC8, and it was successfully used for the segregation of seeded x seedless hybrid genotypes. In a study by Mejía and Hinrichsen (2003), the dominant SCAR marker, called SCF27, was used for selecting seedless progeny in Ruby Seedless x Sultanina hybrids.

Significant progress has been made in the development of molecular markers in grapevines over the past two decades. Linkage groups related to berry size and seedlessness in grapevines were detected in the quantitative trait loci (QTL) maps obtained from different seedless parental combinations (Doligez et al., 2002; Fanizza et al, 2005; Cabezas et al., 2006; Mejía et al., 2007; Costantini et al, 2007, 2008). Two of these were identified on linkage group 18 (LG18), and one on linkage group 4 (LG4) (Dai et al., 2011). Cabezas et al. (2006) developed a genetic map from the F1 combination obtained from the crossbreeding of the conventional, seeded V. vinifera variety "Dominga" and the seedless variety "Autumn Seedless". The QTL map indicated a large QTL region in the linkage group 18 associated with the seedlessness trait, and VMC7F2 was reported as the SSR marker closely associated with this QTL region. It was claimed that the VMC7F2 marker could be used in marker-assisted selection (MAS) for early detection of seedless individuals in breeding. Costantini et al. (2008) confirmed this finding after QTL analyses for berry size and ripening time in grapevines, and identified VMC7F2 as the most closely associated marker with the SdI locus. Followed by further research, papers have been published on the successful use of the VMC7F2 marker in breeding studies on seedlessness. Akkurt et al. (2012) found that the 198 bp allele from the VMC7F2 marker in the "Muscat Hamburg" x "Sultani" hybrid population was closely associated with seedlessness in the F1 generation and stated that it could be used for MAS in the early selection of seedlessness. Karaagaç et al. (2012) indicated that the 198 bp allele and the VMC7F2 marker could be used successfully in the selection of seedless individuals in segregations, and that the progeny size could be reduced to 54%.

Based on their study, involving sequence analyses, sequence characterisation and transcriptional analyses, Mejía et al. (2011) suggested that VvAGL11 was the major functional candidate gene in seedlessness. The researchers reported that VvAGL11 was the major locus for seedlessness in Vitis vinifera varieties, similar to AGL11 from the D-lineage MADS-box family responsible for seed development in different plant species, and developed a series of STS (sequence tagged site) markers associated with this gene. The P3-VvAGL11, which is one of the developed markers, was determined to be used effectively in the early detection of stenospermocarpic seedlessness in grapevines. Bergamini et al. (2013) used the VvAGL11 marker in the F1 genotype of 475 seeded x seedless crosses and indicated that the marker can be used in all seedless phenotypes.

 

NEWLY BRED SEEDLESS GRAPE VARIETIES

There are a total of 67 seedless grape varieties that have been bred since 2000 and recorded in the VIVC database. Of these varieties, 66 are table varieties and one is a wine grape variety. Most of the varieties have been registered in the USA, followed by Turkey, Brazil, China, South Korea and South Africa. The bred varieties and their parents are presented in Table 1.

 

CONCLUSION

The primary traits on which the focus is in the development of new varieties in grapevine-breeding studies are seedlessness, berry size, high quality, resistance to diseases, earliness and lateness, and resistance to stress conditions. In addition to conventional breeding methods in seedless grape breeding, biotechnological approaches have gained importance in recent years. The use of in vivo growth-regulating agents, along with the embryo rescue method, has led to a significant improvement in the survival of abortive ovules.

Especially in seedless grape breeding, the use of genetic markers has gained great importance in its contribution to selection studies. Various researchers have reported that, while VMC7F2, one of the SSR markers, can be used successfully in the early selection of seedless varieties, the p3-VvAGL11 STS marker can be used more effectively in MAS. In this way, the long-term, labour-intensive and costly grapevine-breeding studies can be made shorter and more effective. While studies focusing on the development of new seedless varieties are concentrated mainly in the USA, Turkey, Israel, Italy and China, studies on developing new seedless varieties are ongoing in all countries involved in viticulture business in accordance with their economic priorities.

 

LITERATURE CITED

Adam-Blondon, A.F., Lahogue, F., Bouquet, A., Boursiquot, J.M. & This, P., 2001. Usefulness of two SCAR markers for marker-assisted selection of seedless grapevine cultivars. Vitis 40(3), 147-155.         [ Links ]

Akkurt, M., Çakir, A., Shidfar, M., Mutaf, F. & Söylemezoglu, G., 2012. Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection (MAS) into Muscat of Hamburg χ Sultani Progeny. Turk. J. Biol. 37, 101-105.         [ Links ]

Bergamini, C., Cardone, M.F., Anaclerio, A., Perniola, R., Pichierri, A., Genghi, R., Alba, V., Forleo, L.R., Caputo, A.R., Montemurro, C., Blanco, A. & Antonacci, D., 2013. Validation assay of p3_VvAGL11 marker in a wide range of genetic background for early selection of stenospermocarpy in Vitis vinifera L. Mol. Biotechnol. 54(3), 1021-1030.         [ Links ]

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Date of submission for publication: January 2019
Date of acceptance for publication: March 2019

 

 

* Corresponding author: E-mail address: akkurt@agri.ankara.edu.tr
Acknowledgements:

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