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Water SA

versión On-line ISSN 1816-7950
versión impresa ISSN 0378-4738

Water SA vol.34 no.2 Pretoria feb. 2008


Faecal coliforms, faecal enterococci, Salmonella Typhi and Acanthamoeba spp. UV inactivation in three different biological effluents



NA BeltránI; BE JiménezII

IInstituto de Investigaciones Eléctricas. Calle Reforma 13. Col. Palmira, C.P. 62490, Cuernavaca Morelos. México
IIInstituto de Ingeniería, UNAM. Apdo. Postal 70472; Ciudad Universitaria, Delegación Coyoacán, C.P. 04510, D.F. México





Efficiency of UV-light to inactivate microbial indicators, Salmonella Typhi and Acanthamoeba spp. was studied in three different biological secondary effluents. Even though effluents differed in terms of their total suspended solids content, transmittance and particle size distribution, the UV-light dose required to fulfil WHO agricultural water reuse criteria was the same (30 mW-s/cm2), because the particle content with sizes >40 |xm was similar and very small. Using this dosage, 3 log of Salmonella Typhi and faecal enterococci were also inactivated. To avoid faecal coliform and Salmonella Typhi photoreactivation, the UV dose had to be doubled and in the process 2.5 log of Acanthamoeba spp. were also inactivated. This is interesting because its presence in wastewater, pathogenicity and resistance to conventional disinfection processes has been reported in the literature. Additionally, it was found that the faecal coliforms' inactivation rate constant was the lowest one of all the bacteria studied (Salmonella Typhi and faecal enterococci), suggesting the limitation of this indicator when several kinds of pathogens are present, as is the case in developing countries.

Keywords: amoebae, UV disinfection, indicators, pathogen, risks assessment, wastewater


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Received 31 July 2007
Accepted in revised form 29 January 2008