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SA Orthopaedic Journal

versão On-line ISSN 2309-8309
versão impressa ISSN 1681-150X

SA orthop. j. vol.7 no.4 Pretoria Out./Dez. 2008




Evaluating donor tissue for bacterial contamination at the South African National Tissue Bank



Dr A de VosI; Dr E MaritzI; Prof TLB le RouxII

IRegistrars: Department of Orthopaedic Surgery, University of Pretoria
IIMedical Director of the National Tissue Bank, University of Pretoria and Head of Orthopaedic Surgery at 1 Military Hospital





The internationally accepted method of acquiring allograft bone is to retrieve it in a sterile environment. In South Africa, we are limited by resources and funds, making it impossible to adhere to these standards of retrieval. The purpose of the study is to evaluate the safety of the surgically clean retrieval of allograft bone outside of a theatre set-up.
The study population consisted of all the accepted donors from the beginning of 2003 to September 2008. The donors included in the study (n=749) were tested for microbial growths at various stages of retrieval and processing.
An internal audit was done on the results and the safety of the processes was evaluated. The amount of bacterial contamination of the various samples was used to evaluate the safety of the process.
We concluded that by following a strict protocol for processing and by using mandatory gamma irradiation our allograft is bacteria-free and extremely safe, making our method comparable with international standards.



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1. Komiya K, Nasuno S, Uchiyama K. Status of bone allografting in Japan: Nation-wide survey of bone grafting performed from 1995 through 1999. Cell and tissue banking 2003;4:217-20.         [ Links ]

2. Bohatyrewicz A, Bohatyrewicz R, Mazur R. Bone allograft harvesting following multiorgan procurement. Transplantation Proceedings 2002;34:707-8.         [ Links ]

3. Holt G, Arthur A, Frame D. Human skeletal allograft collection: room for improvement? Scot Med J 2004;49:146-8.         [ Links ]

4. Journeaux SF, Johnson J, Bryce SL. Bacterial contamination rates during bone allograft retrieval. J Arthroplasty 1999;14(6):677-81.         [ Links ]

5. Bohatyrewicz A, Bohatyrewicz R, Dobiecki K. Is retrieval of bone material from multiorgan donors effective enough to cover demand for biostatic bone tissue grafts in Poland? Transplantation Proceedings 2006;38:297-300.         [ Links ]

6. Bohatyrewicz A, Bohatyrewicz R, Klek A. Factors determining the contamination of bone tissue procured from cadaveric and multiorgan donors. Transplantation Proceedings 2006;38:301-4.         [ Links ]

7. Kumta SM, Kendal N, Lee YL. Bacterial colonization of bone allografts related to increased interval between death and procurement: an experimental study in rats. Arch Orthop Trauma Surg 1997;116:496-7.         [ Links ]

8. Sommerville SMM, Johnson N, Bryce SL. Contaminated femoral head allograft: incidence, bacteriology and donor follow up. JBJS Br 1998;80B(2S):135.         [ Links ]

9. Gardner JF, Peel MM. Introduction to sterilization and disinfection. New York: Churchill Livingstone; 1986.         [ Links ]

10. Nather A, Yusuf N, Hilmy N. Radiation in tissue banking. Basic science and clinical applications of irradiated tissue allograft. World Scientific Publishing, Singapore 2007:128.         [ Links ]



Reprint requests:
Dr A de Vos
Department of Orthopaedic Surgery
7th Floor, Pretoria Academic Hospital
Bophelo Road, Pretoria
Tel: +27 12 354-1000; Fax: +27 12 354-2821; Cell: +27 82 926 6732



This article was not submitted to an ethical committee for approval. The content of this article is the sole work of the authors. No benefits of any form have been derived from any commercial party related directly or indirectly to the subject of this article.

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