On-line version ISSN 2224-9435
Print version ISSN 1019-9128
J. S. Afr. Vet. Assoc. vol.86 n.1 Cape Town 2015
Rutendo ManyararaI; Ulf TubbesingI; Minty SoniI; Bruce H. NodenII
IRhino Park Veterinary Clinic, Windhoek, Namibia
IIDepartment of Health Sciences, Polytechnic of Namibia, Namibia
Ehrlichia canis is a major pathogen in dogs throughout Africa, yet it has not been reported in Namibia. The aim of this study was to determine the seroprevalence of canine ehrlichiosis in central Namibia using the ImmunoComb assay (Biogal, Galed Laboratories). The study included 76 dogs that presented to the Rhino Park Veterinary Clinic in the north-western suburb of Khomasdal, Windhoek, Namibia, as well as 30 stray dogs from the Windhoek branch of the Society for the Prevention of Cruelty to Animals. Of the 106 dogs tested, 53.8% were seropositive at titres > 1:80. Dogs that presented with symptoms of E. canis infection had a significantly higher seroprevalence (86.6%) compared with apparently healthy dogs (41.6%) (P = 0.00). Location of habitation was significant (P < 0.017), with a high percentage of dogs exposed to E. canis living in the northern or north-western part of Windhoek. As the first study to serologically establish E. canis as a major pathogen in dogs in central Namibia, it is notable that the highest proportion of seropositive dogs came from low-income areas. Further investigation is necessary to describe the ecology of this important tick-borne pathogen of companion animals in Namibia.
Canine ehrlichiosis is caused by Ehrlichia canis, a rickettsial Gram-negative species in the family Anaplasmataceae (Dumler et al. 2007). Throughout the world, E. canis is transmitted by the brown dog tick, Rhipicephalus sanguineus (Harrus & Waner 2011; Nicholson et al. 2010), and is one of four species in the genus (E. canis, Ehrlichia chaffeensis, Ehrlichia ewingii and Ehrlichia ruminantium) that cause serious disease in animals and humans (Dumler et al. 2007; Louw, Allsopp & Meyer 2005; Murphy et al. 1998). Ehrlichia canis infection in dogs has been reported widely in Africa (Kamani et al. 2013; Kelly, Eoghain & Raoult 2004; Matthewman et al. 1993; Pretorius & Kelly 1998; Socolovschi et al. 2012), but not in Namibia specifically. Therefore, the aim of this study was to determine the seroprevalence of canine ehrlichiosis in central Namibia using a point-of-care assay.
Materials and methods
The study was conducted at the Rhino Park Veterinary Clinic in Khomasdal, a north-western suburb of Windhoek, Namibia. Data were collected over a period of 8 weeks from dogs that presented to the clinic as well as from stray dogs under the care of the Windhoek branch of the Society for the Prevention of Cruelty to Animals (SPCA). In all cases, participation was voluntary and depended on ease of handling the patient and written informed consent from the patient's direct owner or guardian. The study protocol was approved by the Namibian Ministry of Agriculture, Water and Forestry.
At the clinic, veterinarians evaluated dogs for presenting with symptoms such as inappetence, lethargy and emaciation. Blood samples (2 mL - 5 mL) were obtained from central sites (e.g. the jugular vein) by venipuncture. After initial evaluation, these dogs were assigned to either of two groups, (1) those suspected of E. canis infection or (2) those with non-specific symptoms or that came to the clinic for elective procedures. At inclusion in the study, the sex and age of the dog were noted. Clinic veterinarians also evaluated 30 stray dogs from the local SPCA during the same period to provide another estimate of the potential prevalence of the disease in central Namibia.
The ImmunoComb kit (Biogal, Galed Laboratories, Israel), a solid-phase dot enzyme-linked immunosorbent assay (ELISA) based on a crude antigen of E. canis (Israeli strain), was used according to the manufacturer's instructions to detect IgG-specific antibodies. The manufacturer's cut-off value for positive samples was a titre > 1:80. Reported sensitivity and specificity of this test are 96% and 87%, respectively, when compared with the 'gold standard' immunofluorescent antibody test for Nigerian strains of E. canis (Okewole & Adejinmi 2009). Similarly, sensitivity and specificity for Middle Eastern strains of E. canis are reported at 86% and 98%, respectively, with this ELISA (Harrus et al. 2002). Serum samples were assayed immediately after processing and the remainder of each sample was stored at -20 °C for further analysis.
Data were analysed using the PASW Statistics 18 (SPSS Inc, Chicago, IL) software package. Chi-square and Fisher exact tests were used to compare proportions between groups and f-tests and Analysis of variance (ANOVA) were used for comparisons of continuous variables. Age of animals was analysed in three groups: < 1 year, between 1 and 8 years, and > 8 years. A P-value of less than 0.05 was considered statistically significant for all tests.
A total of 106 dogs from various areas of Windhoek and the surrounding Khomas region were evaluated. Of these, 76 were dogs that visited the clinic and 30 were apparently healthy strays from the local SPCA. Ehrlichia canis infection was suspected for the majority (86.2%) of the clinic-evaluated dogs as they presented with inappetence, lethargy and emaciation. The majority of dogs sampled were males (53.5%) and younger than 8 years (92.7%) (Table 1). Ticks were found on 17 (22.4%) of the clinic-evaluated dogs, with 13 (76.5%) having clinical signs as opposed to 4 (23.5%) without clinical signs.
Of the 106 dogs tested, 57 (53.8%) tested seropositive for antibodies against E. canis at titres > 1:80. Dogs with symptoms of E. canis had a higher seroprevalence (86.6%) than dogs without symptoms (42.3%) or stray dogs (40.0%) (P = 0.00).
Seroprevalence was similar for male and female dogs and did not differ significantly between the age groups (Table 1). Location of habitation was significant, with a high proportion of seropositive dogs coming from the northern or northwestern parts of Windhoek (Khomasdal and Katatura areas) (Table 1). Ticks were found on more dogs from the northern or north-western areas (32.1%) than on dogs from other areas (15.4%), but the difference was not significant (P < 0.150).
This is the first serological study to establish E. canis as a major pathogen in dogs in central Namibia. The high seroprevalence (53.8%) of E. canis in the sample of Namibian dogs is comparable to results from studies in rural Zimbabwe (34%) (Kelly et al. 2004) and urban South Africa (42%) (Pretorius & Kelly 1998). The highest proportion of seropositive dogs came from the lower-income areas of Khomasdal and Katatura, which reflects a similar trend to that reported in Bloemfontein, South Africa (Pretorius & Kelly 1998). The only described vector of canine ehrlichiosis, Rhipicephalus sanguineus (the brown dog tick), is the principal tick species found on dogs throughout central and southern Namibia (Matthee et al. 2010). With a higher prevalence of ticks found on dogs from lower-income areas, this pattern may be due to a lack of access to tick control (Pretorius & Kelly 1998).
Although the involvement of only one veterinary clinic and the limited timescale and sample size may introduce several limitations, this study demonstrates that dogs in central Namibia are frequently exposed to Ehrlichia species, the most likely being E. canis. Based on the serological detection, further molecular investigation of Namibian dogs and their ticks is required to verify the presence of Ehrlichia species.
The authors wish to acknowledge the assistance of those who have provided invaluable assistance in this study. R. Manyarara acknowledges the assistance of Kathleen Tennant (MRCVS), Dr Linda Matthewman, and Alice Schahaff (Biogal, Galed Laboratories, Israel). The authors would also like to thank the Permanent Secretary of the Namibian Ministry of Agriculture, Water and Forestry and the Directorate of Veterinary Services for their permission to publish this paper as well as their helpful suggestions. B.H.N. was partially supported by the Oklahoma Agricultural Experiment Station (OKL-02909).
The authors declare that they have no financial or personal relationships that may have inappropriately influenced them in writing this article.
R.M. (Rhino Park Veterinary Clinic) and U.T. (Rhino Park Veterinary Clinic) were the project leaders and were responsible for the experimental design. R.M., U.T. and M.S. (Rhino Park Veterinary Clinic) performed the experimental procedures and were responsible for the acquisition and testing of samples. B.H.N. (Polytechnic of Namibia) analysed the data and organised the original draft of the manuscript. All authors contributed to the writing of the manuscript.
Dumler, J.S., Madigan, J.E., Pusterla, N. & Bakken, J.S., 2007, 'Ehrlichiosis in humans: Epidemiology, clinical presentation, diagnosis, and treatment', Clinical Infectious Diseases 45(S1), S45-S51. http://dx.doi.org/10.1086/518146 [ Links ]
Harrus, S., Alleman, A.R., Bark, H., Mahan, S.M. & Waner, T., 2002. 'Comparison of three enzyme-linked immunosorbant assays with the indirect immunofluorescent antibody test for the diagnosis of canine infection with Ehrlichia cam's', Veterinary Microbiology 86(4), 361-368. http://dx.doi.org/10.1016/S0378-1135(02)00022-6 [ Links ]
Harrus, S. & Waner, T., 2011, 'Diagnosis of canine monocytotropic ehrlichiosis (Ehrlichia cam's): An overview', Veterinary Journal 187(3), 292-296. http://dx.doi.org/10.1016/j.tvjl.2010.02.001 [ Links ]
Kamani, J., Baneth, G., Mumcuoglu, K.Y., Waziri, N.E., Eyal, O., Guthmann, Y. et al., 2013, 'Molecular detection and characterization of tick-borne pathogens in dogs and ticks from Nigeria', PLoS Neglected Tropical Diseases 7(3), e2108. http://dx.doi.org/10.1371/journal.pntd.0002108 [ Links ]
Kelly, P.J., Eoghain, G.N. & Raoult, D., 2004, 'Antibodies reactive with Bartonella henselae and Ehrlichia canis in dogs from the communal lands of Zimbabwe', Journal of the South African Veterinary Association 75(3), 116-120. http://dx.doi.org/10.4102/jsava.v75i3.465 [ Links ]
Louw, M., Allsopp, M.T. & Meyer, E.C., 2005, 'Ehrlichia ruminantium, an emerging human pathogen - a further report', South African Medical Journal 95(12), 948. [ Links ]
Matthee, S., Lovely, C., Gaugler, A., Beeker, R., Venter, H.R. & Horak, I.G., 2010, 'Ixodid ticks on domestic dogs in the Northern Cape Province of South Africa and in Namibia', Journal of the South African Veterinary Association 81(2), 126-128. http://dx.doi.org/10.4102/jsava.v81i2.125 [ Links ]
Matthewman, L.A., Kelly, P.J., Mahan, S.M., Semu, D., Tagwira, M., Bobade, P.A. et al., 1993, 'Western blot and indirect fluorescent antibody testing for antibodies reactive with Ehrlichia canis in sera from apparently healthy dogs in Zimbabwe', Journal of the South African Veterinary Association 64(3), 111-115. [ Links ]
Murphy, G.L., Ewing, S.A., Whitworth, L.C., Fox, J.C. & Kocan, A.A., 1998, 'A molecular and serologic survey of Ehrlichia canis, E. chaffeensis, and E. ewingii in dogs and ticks from Oklahoma', Veterinary Parasitology 79(4), 325-339. http://dx.doi.org/10.1016/S0304-4017(98)00179-4 [ Links ]
Nicholson, W.L., Allen, K.E., McQuiston, J.H., Breitschwerdt, E.B. & Little, S.E., 2010, 'The increasing recognition of rickettsial pathogens in dogs and people', Trends in Parasitology 26(4), 205-212. http://dx.doi.org/10.1016/j.pt.2010.01.007 [ Links ]
Okewole, E.A. & Adejinmi, J.O., 2009, 'Comparison of two clinic-based immunoassays with the immunofluorescence antibody test for the field diagnosis of canine monocytic ehrlichiosis', Acta Microbiologica et Immunologica Hungarica 56(2), 145-155. http://dx.doi.org/10.1556/AMicr.56.2009.2.3 [ Links ]
Pretorius, A.M. & Kelly, P.J., 1998, 'Serological survey for antibodies reactive with Ehrlichia canis and E. chaffeensis in dogs from the Bloemfontein area, South Africa', Journal of the South African Veterinary Association 69(4), 126-128. http://dx.doi.org/10.4102/jsava.v69i4.840 [ Links ]
Socolovschi, C., Gomez, J., Marié, J.L., Davoust, B., Guigal, P.M., Raoult, D. et al., 2012, 'Ehrlichia canis in Rhipicephalus sanguineus ticks in the Ivory Coast, Ticks and Tick-borne Diseases 3(5-6), 411-U3. http://dx.doi.org/10.1016/j.ttbdis.2012.10.005 [ Links ]
Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater Campus
74078 Oklahoma, United States of America
Received: 20 Jan. 2015
Accepted: 10 Apr. 2015
Published: 01 June 2015