versión On-line ISSN 2224-9435
versión impresa ISSN 1019-9128
J. S. Afr. Vet. Assoc. vol.85 no.1 Cape Town ene. 2014
Gert J. VenterI, II; Karien LabuschagneI, III; Solomon N.B. BoikanyoI; Liesl MoreyIV
IParasites, Vectors & Vector-borne Diseases Programme, Agricultural Research Council-Onderstepoort Veterinary Institute, Pretoria, South Africa
IIDepartment of Veterinary Tropical Diseases, University of Pretoria, South Africa
IIIDepartment of Zoology & Entomology, University of Pretoria, South Africa
IVAgricultural Research Council-Biometry Unit, Pretoria, South Africa
The use of insect repellents to reduce the attack rate of Culicoides species (Diptera: Ceratopogonidae) should form part of an integrated control programme to combat African horse sickness and other diseases transmitted by these blood-feeding midges. In the present study the repellent effects of a commercially available mosquito repellent, a combination of citronella and lemon eucalyptus oils, on Culicoides midges was determined. The number of midges collected with two 220 V Onderstepoort traps fitted with 8 W 23 cm white light tubes and baited with peel-stick patches, each containing 40 mg of active ingredient, was compared with that of two unbaited traps. Two trials were conducted and in each trial the four traps were rotated in two replicates of a 4 x 4 randomised Latin square design. Although more midges were collected in the baited traps, the mean number in the baited and unbaited traps was not significantly different. This mosquito repellent did not influence either the species composition or the physiological groups of Culicoides imicola Kieffer. The higher mean numbers in the baited traps, although not statistically significant, may indicate that this mosquito repellent might even attract Culicoides midges under certain conditions.
Small (< 3 mm) blood-feeding flies of the genus Culicoides (Diptera: Ceratopogonidae) are associated worldwide with the transmission of several pathogens to a variety of hosts (Borkent 2005; Meiswinkel, Venter & Nevill 2004). Of the more than 66 viruses (Borkent 2005) isolated from Culicoides midges, African horse sickness virus (AHSV) is probably the most devastating to South Africa owing to its effect on the horse industry. Despite the widespread use of live-attenuated African horse sickness (AHS) vaccines, more than 1500 horses in South Africa have succumbed to AHS since 2005 (African Horse Sickness Trust 2012). This relatively high death rate can partly be ascribed to the large numbers of vectors, Culicoides species, present in the endemic AHS areas of South Africa. Culicoides imicola Kieffer (C. imicola), the most abundant livestock-associated Culicoides species, is considered to be a proven vector of AHSV (Meiswinkel et al. 2004; Mellor, Boorman & Baylis 2000). Under favourable conditions, more than a million C. imicola females can be collected overnight in a single light trap near livestock (Meiswinkel et al. 2004). Although these traps intercept only a relatively small percentage of the active blood-seeking females (Meiswinkel et al. 2004) and do not always reflect the true biting rate on livestock (Carpenter et al. 2008; Gerry et al. 2009; Scheffer et al. 2012; Viennet et al. 2011), these numbers give an indication of the potentially high attack rate that horses and other livestock can be exposed to in endemic areas. This perceived high attack rate emphasises the need for supportive control measures, for example the use of insect repellents and/or insecticides, in addition to vaccination, as part of an integrated control programme. Repellents and insecticides can be applied directly to livestock and/or to their immediate surroundings, for example gauze coverings of stable windows (Meiswinkel, Baylis & Labuschagne 2000).
Since the 1960s increasing concerns about the environmental impact of insecticides and an increased resistance in pest species have resulted in a decline in the number of agents available for pest management. An additional shortcoming of insecticides is that infected midges may be able to feed and potentially transmit pathogens before being incapacitated (Mullens et al. 2000).
In addition to the use of insecticides, repellents can be used to reduce the Culicoides attack rate and potential transmission of pathogens (White & Evans 2002). The evaluation of repellents against Culicoides midges is hampered by their small size (3 mm to 4 mm) and mainly nocturnal activity. To date in South Africa only two compounds have been shown to repel Culicoides midges from a light trap when applied to the polyester mesh surrounding the trap. These products are a 15% N,N-diethyl-3-methylbenzamide (DEET) (Page et al. 2009) and a mixture of octanoic acid (C8), nonanoic acid (C9) and decanoic acid (Cl0) (Venter et al. 2011), fatty acids that occur naturally in a variety of plants and on the surface of human skin.
The apparent repellent effect of citronella on mosquitoes and other pest insects was already observed in 1901 (Granett 1940) and numerous reports on the efficacy of citronella or citronella-derived components as repellents against a variety of arthropods have been published since (Fradin & Day 2002; Kongkaew et al. 2011; Osmani, Anees & Naidu 1972; Revay et al. 2013; Tawatsin et al. 2001, 2006). Depending on the mode of application, additives and test procedures, the efficacy varies between species (Kongkaew et al. 2011; Osmani Anees & Naidu 1972). It has been found that some plant-derived repellents, including citronella, can give up to 9 h protection against mosquitoes and blackflies (Tawatsin et al. 2006). The principle constituent of citronella, Citronellol (3,7-dimethyl-6-octen-8-ol), is a volatile unsaturated aldehyde (terpene) with a characteristically strong, sweet smell (Botha & McCrindle 2000).
Because certain plant-derived mosquito repellents are regarded as safe for use on vertebrates, studies in Israel have begun to focus on their use to protect livestock against C. imicola (Braverman & Chizov-Ginzburg 1998; Braverman, Chizov-Ginzburg & Mullens 1999; Braverman, Wegis & Mullens 2000). Braverman et al. (1999) found that a plant-derived mosquito repellent based on the oil of Eucalyptus maculata can attract large numbers of C. imicola. However, other studies have shown that it will repel the Scottish species, Culicoides impunctatus Goetghebeur, from humans in the field (Trigg 1996) and the North American species, Culicoides variipennis Coquillett, from humans in the laboratory (Trigg & Hill 1996). In Spain, it was shown that citronella-based products will repel biting flies (Simulium and Culicoides species) from fumigated bird nests for up to three days (Martínez-de la Puente et al. 2009). These findings highlight the fact that the genus Culicoides is biologically highly diverse and that extrapolation of data from one species to another is not recommended (Tabachnick 1992).
Plant-derived repellents, if effective, may provide a cheap, environmentally safe and practical alternative to protect horses and other livestock against midges. In the present study, light traps were used to determine the potential repellent efficacy of a commercially available mosquito repellent against Culicoides species - a combination of citronella and lemon eucalyptus oils. This data may help to identify compounds that can be further evaluated for their use on livestock and will enable researchers to provide scientific advice to veterinarians and stock owners on the effectiveness of these products.
Material and methods
To determine the repellent effects of a combination of citronella and lemon eucalyptus oil, commercially available (Lifecare Medical Instruments Co., Ltd., Taiwan) peel-stick patches (diameter: 40 mm x 1 mm x 1.0 g) were used. Each patch, containing 40 mg active ingredient, is individually sealed in an aluminium package and can, according to the manufacturer, give up to 12 h protection against mosquitoes when stuck to clothing. As in previous studies conducted on repellents in South Africa (Venter et al. 2011), the number of midges collected with two 220 V down-draught Onderstepoort light traps baited with the repellent was compared with that of two unbaited control traps. A new patch was stuck on to the top of a light trap each night immediately before trapping was started. To reduce the relatively strong attraction of the light trap, the black light tubes were replaced with 8 W, 23 cm white light tubes (Venter & Hermanides 2006). Moths and larger insects were excluded by polyester netting (hole size 2 mm), which was placed around the entrance portals of the traps.
Two trials were conducted. In the first, trapping was carried out for eight nights in autumn, between 11 May 2009 and 03 June 2009. The second was conducted for eight nights in the height of summer between 27 January 2010 and 20 February 2010. Light traps were suspended 1.8 m above ground-level under the eaves of open-sided barns housing between 20 and 30 cattle; each were at the Agricultural Research Council-Onderstepoort Veterinary Institute (ARC-OVI) (25°39'S 28°11'E; 1219 m above sea level). At all four sites, the traps were hung as close to the cattle as practicably possible. To minimise interference between traps, sites were located at least 15 m apart (Venter et al. 2012). To eliminate any effects due to site or occasion, trap treatments at the four collection sites were alternated in three replicates of a 4 × 4 randomised Latin square design (Perry, Wall & Greenway 1980). Light trap operating procedure was conducted as previously described (Venter et al. 2009).
After retrieval in the morning, insects were transferred to 80% ethanol. Large collections were sub-sampled (Van Ark & Meiswinkel 1992) and Culicoides midges were counted, sexed and sorted to species level. Females were classified according to their abdominal pigmentation (Dyce 1969), these were: unpigmented (nulliparous); pigmented (parous); gravid (with eggs visible in the abdomen); or freshly blood-fed. The numbers of males and other insects were also recorded.
Analysis of variance (ANOVA) was used to differentiate between trap treatment effects at the 5% level. Treatment means were separated using Fisher's protected t-test least significant difference (LSD) at the 5% significance (VSN International 2012). Evenness in species distribution and diversity for each treatment site was calculated with the Shannon Wiener index (Al Young Studios 2012). Species abundance in the different treatments was compared using linear regression GraphPadInStat Version 3 (GraphPad, USA).
Materials used in the experiment posed no health risk to researchers and no animals were harmed. The study was done as part of a project on National Assets at the ARC-OVI (project OV 7/03/P002-Insect Collection).
In the first trial (11 May 2009 to 03 June 2009), 144 323 Culicoides midges were collected in 32 collections made over eight nights. Taking into account the substantial day-to-day variation in the numbers per trap per night, the higher mean in the 16 collections made with the two repellent traps (4660.6) was not significantly greater (p = 0.559) than the mean of that of the two unbaited control traps (4359.8) (Table 1).
Nine different Culicoides species were collected in the control and ten in the baited traps. The most abundant species, representing 95.0% in the control and 95.5% in the baited traps, was C. imicola (Table 1). The higher mean number of C. imicola in the baited traps (4449.3) was not significantly (p = 0.662) different from that (4359.8) in the control traps (Table 1). The second most abundant species in both trapping regimens was Culicoides enderleini Cornet and Brunhes (C. enderleini). This species represented 3.8% of the midges in the control traps and 4.0% of the midges collected in the baited traps (Table 1). The higher mean number in the baited traps (188.7) was not significantly different (p = 0.534) from that of the control traps (165.8) (Table 1).
A breakdown of the physiological stages of C. imicola showed that 99.2% (control traps) and 99.1% (baited traps) were females actively seeking a blood meal. The mean numbers of unpigmented (nulliparous) females collected in the baited (3309.5) and control (3029.8) traps were not significantly different (p = 0.563). Similarly the mean numbers of pigmented (parous) females in the baited (1098.4) and control traps (1079.7) were not significantly different (p = 0.932). Freshly blood-fed females (0.1%), gravid females (0.5%) and males (0.2%) represented 0.8% of the total number of C. imicola collected in the control traps, whilst in the baited traps blood-fed females (0.1%), gravid females (0.6%) and males (0.3%) represented 1.0% of the total number (Table 1). There was no significant difference (p = 0.889) in the numbers of other insects collected (Table 1).
In the second trial in summer (27 January 2010 to 20 February 2010), 123 941 Culicoides midges were collected in 32 collections. Of these, 59.5% were in the two baited traps. As in the previous trial, the higher mean collected with the baited traps (4605.8) was not significantly different (p = 0.481) from that in the control traps (3139.6) (Table 1).
Whilst 19 different Culicoides species were collected in the control traps, 22 were present in the baited traps (Table 1). Culicoides imicola, representing 97.9% in both regimens, was the dominant species with no significant difference (p = 0.500) in the mean numbers in baited traps (4509.2) or control traps (3073.3) (Table 1). Also, for C. enderleini (the second most dominant species) the higher mean in the baited traps (30.4) was not significantly (p = 0.114) different from that of the control traps (10.4) (Table 1).
Active blood-seeking C. imicola females represented 90.7% and 92.0% of the midges in the control traps and baited traps respectively. There were no significant differences in either the mean number of nulliparous (p = 0.179) or parous (p = 0.130) C. imicola collected (Table 1). Freshly blood-fed females (1.0%), gravid females (0.6%) and males (7.8%) represented 9.4% of the totals in the control, whilst in the baited traps, blood-fed females (0.7%), gravid females (0.3%) and males (6.9%) represented 7.9% of the total numbers (Table 1). As in the first trial, there was no significant difference (p = 0.495) in the numbers of other insects collected (Table 1).
In both trials a strong linear correlation (R2 = 100%) was found in the proportion of different species collected with the control trap and repellent trap. Species diversity and evenness as reflected by the Shannon-Weiner index, which describes the evenness in distribution of species abundances taking sample size into account, was nearly identical between treatments (Table 1).
In the present evaluation using light traps, a combination of citronella and lemon eucalyptus oil did not appear to have any repellent effect on Culicoides midges. Females of C. imicola looking for a blood meal were the dominant grouping in both trapping regimens and no significant differences were found in the different physiological stages. Higher numbers as well as a larger number of species were collected with the baited traps. However, owing to the substantial day-to-day variation in the numbers these differences were not statistically significant. These results are in agreement with those of Page et al. (2009), who found that higher mean numbers of Culicoides midges, including C. imicola, were collected with light traps baited with 0.6% citronella oil.
Owing to the hole size (2 mm) of the netting used on the Onderstepoort trap, mosquitoes and insects larger than Culicoides midges were excluded from the trap, so the repellent effect of this combination of oils on the numbers of these insects could not be evaluated. The higher mean number of insects other than Culicoides midges collected in the baited traps was not significantly different from those of the controls.
In evaluating repellents, the influence of the ambient temperature, wind speed and other factors that affect the dispersal capacity of the repellent must be taken into consideration. During May to June the mean numbers collected with the baited traps was 1.1 times higher than those collected in the control traps. During the warmer period of January to February the baited traps collected on average 1.5 more midges than the control traps.
A shortcoming of the present evaluation is that the relatively strong attraction of the light trap for Culicoides species coupled with the strong downdraught of the fan could have counteracted any repellent effect of the compound tested. Attraction of insects to a light source is somewhat artificial and it may not be comparable to that of a warm-blooded host animal. It should be borne in mind that on mammals the far more complex nature of natural mixtures and concentrations of various organic components found on the skin might interact with other chemicals to increase or decrease attractiveness of a product (Bosch, Geier & Boeckh 2000). Despite these shortcomings, light traps were used efficiently to determine the repellent effect of DEET (Page et al. 2009) and a mixture of organic fatty acids (Venter et al. 2011) for Culicoides midges.
The apparent repellence of citronella for various species of mosquitoes and other pest insects has been documented since 1901 (Granett 1940). Even with some encouraging results having been published (Martínez-de la Puente et al. 2009; Tawatsin et al. 2006), citronella is generally rated as less effective than repellents with synthetic active ingredients (Kongkaew et al. 2011). Despite this, it has long been used in a number of commercial preparations (Curtis et al. 1987). In a review by Fradin and Day (2002) it is stated that none of thousands of plant extracts were able to repel mosquitoes for more than 1 h and 30 min and that most, regardless of their active ingredients and formulations, gave very short-lived protection, ranging from 3 min to 20 min.
In endemic situations like South Africa, vaccination will always remain the key control strategy for diseases caused by viruses transmitted by Culicoides midges. However, effective repellents can help in decreasing Culicoides attack rates (White & Evans 2002). In order to develop and evaluate repellents successfully an understanding of the molecular basis of insect olfaction will be essential (Bohbot & Dickens 2010; Logan & Birkett 2007). Repellents can either be applied directly on to the animals and/or to their immediate surroundings (Meiswinkel et al. 2000). To date, only two repellents, DEET (Page et al. 2009) and a combination of organic fatty acids (Venter et al. 2011), have been found to effectively repel Culicoides midges as determined with light traps under field conditions in South Africa. In an effort to control viral diseases transmitted by Culicoides midges it will be worthwhile to test these products for efficiency and safety on livestock. The evaluation of a great number of potential repellents on various livestock species will, however, be labour intensive, expensive and in certain instances, unethical. Light trap results can be used to screen products before their evaluation on livestock.
In the present study no evidence could be found to support the use of a combination of citronella and lemon eucalyptus oil as a repellent for Culicoides midges. The higher mean numbers, although not significant, collected with the baited traps may indicate that citronella, under certain conditions, might even attract Culicoides midges. An apparent attraction effect of citronella for C. imicola was also found in previous studies (Braverman et al. 1999; Page et al. 2009).
The authors would like to thank the ARC-OVI for supporting this work. Also, thank Dr Errol Nevill and Dr Truuske Gerdes for constructive comments on earlier drafts of this manuscript. We also thank the anonymous referees for their valuable input for improving the quality of this manuscript.
The authors declare that they have no financial or personal relationship(s) that may have inappropriately influenced them in writing this article.
G.J.V. (Agricultural Research Council-Onderstepoort Veterinary Institute) was responsible for the project design. K.L. (Agricultural Research Council-Onderstepoort Veterinary Institute) did all the Culicoides species analyses and age grading of the collections. S.N.B.B. (Agricultural Research Council-Onderstepoort Veterinary Institute) was responsible for the collection of the Culicoides midges and the rotation of the light traps. L.M. (Agricultural Research Council-Biometry Unit) was responsible for most of the statistical analyses. G.J.V. compiled the data and the draft of the manuscript.
Al Young Studios, 2012, Biodiversity calculator, viewed 03 January 2012, from http://alyoung.com/labs/biodiversity_calculator.html [ Links ]
Bohbot, J.D. & Dickens, J.C., 2010, 'Insect repellents: Modulators of mosquito odorant receptor activity', Plos ONE 5, 8 e12138. [ Links ]
Borkent, A., 2005, 'The biting midges, the Ceratopogonidae (Diptera)', in W.C. Marquardt (ed.), Biology of disease vectors, 2nd edn., pp. 113-126, Elsevier Academic Press, San Diego. [ Links ]
Bosch, O.J., Geier, M. & Boeckh, J., 2000, 'Contribution of fatty acids to olfactory host finding of female Aedes aegypti', Chemical Senses 25, 323-330. http://dx.doi.org/10.1093/oxfordjournals.chemse.a014042 [ Links ]
Botha, B.M. & McCrindle, C.M.E., 2000, 'An appropriate method for extracting the insect repellent citronellol from an indigenous plant (Pelargonium graveolens L'Her) for potential use by resource-limited animal owners', Journal of the South African Veterinary Association 71, 103-105. http://dx.doi.org/10.4102/jsava.v71i2.688 [ Links ]
Braverman, Y. & Chizov-Ginzburg, A., 1998, 'Duration of repellency of various synthetic and plant-derived preparations for Culicoides imicola, the vector of African horse sickness virus', Archives of Virology Supplement 14,165-174. [ Links ]
Braverman, Y., Chizov-Ginzburg, A. & Mullens, B.A., 1999, 'Mosquito repellent attracts Culicoides imicola (Diptera: Ceratopogonidae)', Journal of Medical Entomology 36, 113-115. [ Links ]
Braverman, Y., Wegis, M.C. & Mullens, B.A., 2000, 'Response of Culicoides sonorensis (Diptera: Ceratopogonidae) to 1-octen-3-ol and three plant-derived repellent formulations in the field', Journal of the American Mosquito Control Association 16, 158-163. [ Links ]
Carpenter, S., Szmaragd, C., Barber, J., Labuschagne, K., Gubbins, S. & Mellor, P., 2008, 'An assessment of Culicoides surveillance techniques in northern Europe: Have we underestimated a potential bluetongue virus vector?', Journal of Applied Ecology 45, 1237-1245. [ Links ]
Curtis, C.F., Lines, J.D., Ijumba, J., Callaghan, A., Hill, N. & Karimzad, M.A., 1987, 'The relative efficacy of repellents against mosquito vectors of disease', Medical and Veterinary Entomology 1, 109-119. http://dx.doi.org/10.1111/ij.1365-2915.1987.tb00331.x [ Links ]
Dyce, A.L., 1969, 'The recognition of nulliparous and parous Culicoides (Diptera: Ceratopogonidae) without dissection', Journal of the Australian Entomological Society 8, 11-15. http://dx.doi.org/10.1111/j.1440-6055.1969.tb00727.x [ Links ]
Gerry, A.C., Sarto I Monteys, V., Moreno Vidal, J.O., Francino, O. & Mullens, B.A., 2009, 'Biting rates of Culicoides midges (Diptera: Ceratopogonidae) on sheep in north eastern Spain in relation to midge capture using UV light and carbon dioxide-baited traps', Journal of Medical Entomology 46, 615-624. http://dx.doi.org/10.1603/033.046.0329 [ Links ]
Granett, P., 1940, 'Studies of mosquito repellents II Relative performance of certain chemicals and commercially available mixtures as mosquito repellents', Journal of Economic Entomology 32, 566-572. [ Links ]
Kongkaew, C., Sakunrag, I., Chaiyakunapruk, N. & Tawatsin, A., 2011, 'Effectiveness of citronella preparations in preventing mosquito bites: Systematic review of controlled laboratory experimental studies', Tropical Medicine and International Health 16, 802-810. http://dx.doi.org/10.1111/j.1365-3156.2011.02781.x [ Links ]
Martínez-de la Puente, J., Merino, S., Lobato, E., Rivero-de Aguilar, J., del Cerro, S. & Ruiz-de-Castaneda, R., 2009, 'Testing the use of a citronella-based repellent as an effective method to reduce the prevalence and abundance of biting flies in avian nests', Parasitological Research 104, 1233-1236. http://dx.doi.org/10.1007/s00436-009-1353-9 [ Links ]
Meiswinkel, R., Baylis, M. & Labuschagne, K., 2000, 'Stabling and the protection of horses from Culicoides bolitinos (Diptera: Ceratopogonidae), a recently identified vector of African horse sickness', Bulletin of Entomological Research 90, 509-515. http://dx.doi.org/10.1017/S0007485300000626 [ Links ]
Meiswinkel, R., Venter, G.J. & Nevill, E.M., 2004, 'Vectors: Culicoides spp.', in J.A.W. Coetzer & R.C. Tustin (eds.), Infectious diseases of livestock, 2nd edn., pp. 93-136, Oxford University Press, Cape Town. [ Links ]
Mellor, P.S., Boorman, J. & Baylis, M., 2000, 'Culicoides biting midges: Their role as arbovirus vectors', Annual Review of Entomology 45, 307-340. http://dx.doi.org/10.1146/annurev.ento.45.1.307 [ Links ]
Mullens, B.A., Velten, R.K., Gerry, A.C., Braverman, Y. & Endris, R.G., 2000, 'Effects of permethrin and pirimiphos-methyl applied to cattle on feeding and survival of Culicoides sonorensis (Diptera: Ceratopogonidae)', Medical and Veterinary Entomology 14, 313-320. http://dx.doi.org/10.1046/j.1365-2915.2000.00243.x [ Links ]
Osmani, Z., Anees, I. & Naidu, M.B., 1972, 'Insect repellent creams from essential oils', Pesticides 6, 19-21. [ Links ]
Page, P.C., Labuschagne, K., Nurton, J.P., Venter, G.J. & Guthrie, A., 2009, 'Duration of repellency of N,N-diethyl-3-methylbenzamide, citronella oil and cypermethrin against Culicoides species when applied to polyester mesh', Veterinary Parasitology 163, 105-109. http://dx.doi.org/10.1016/jj.vetpar.2009.03.055 [ Links ]
Perry, J.N., Wall, C. & Greenway, A.R., 1980, 'Latin square designs in field experiments involving sex attractants', Ecological Entomology 5, 385-396. http://dx.doi.org/10.1111/j.1365-2311.1980.tb01162.x [ Links ]
Revay, E.E., Junnila, A., Xue, R-D., Kline, D.L., Bernier, U.R., Kravchenko, V.D., Qualls, W.A., Ghattas, N. & Müller, G.C., 2013, 'Evaluation of commercial products for personal protection against mosquitoes', Acta Tropica 125, 226-230. http://dx.doi.org/10.1016/j.actatropica.2012.10.009 [ Links ]
Scheffer, E.G., Venter, G.J., Labuschagne, K., Page, P.C., Mullens, B.A., McLachlan, N.J., Osterrieder, N. & Guthrie, A.J., 2012, 'Comparison of two trapping methods for Culicoides biting midges and determination of African horse sickness virus prevalence in midge populations at Onderstepoort, South Africa,' Veterinary Parasitology 185, 265-273. http://dx.doi.org/10.1016/jj.vetpar.2011.09.037 [ Links ]
Tabachnick, W.J., 1992, 'Genetics, population genetics, and evolution of Culicoides variipennis: Implications for bluetongue virus transmission in the USA and its international impact', in T.E. Walton & B.I. Osburn (eds.), Bluetongue, African horse sickness and related orbiviruses, pp. 262-270. CRC Press Inc., Boca Raton. [ Links ]
Tawatsin, A., Thavara, U., Changsang, U., Chavalittumrong, P., Boonraud, T., Wongsinkongman, P., Banshidi, J. & Mulla, M.S., 2006, 'Field evaluation of DEET, Repel Care®, and three plant based essential oil repellents against mosquitoes, back flies (Diptera: Simuliidae), and land leeches (Arhynchobdellida: Haemadipsidae) in Thailand', Journal of the American Mosquito Control Association 22, 306-313. http://dx.doi.org/10.2987/8756-971X(2006)22[306:FEODRC]2.0.CO;2 [ Links ]
Tawatsin, A., Wratten, S.D., Scott, R.R., Usavadee, T. & Techadamrongsin, Y., 2001, 'Repellency of volatile oils from plants against three mosquito vectors', Journal of Vector Ecology 26, 76-82. [ Links ]
Trigg, J.K., 1996, 'Evaluation of a eucalyptus-based repellent against Culicoides impunctatus (Diptera: Ceratopogonidae) in Scotland', Journal of the American Mosquito Control Association 12, 329-330. [ Links ]
Trigg, J.K. & Hill, N., 1996, 'Laboratory evaluation of a eucalyptus-based repellent against four biting arthropods', Phytotherapy Research 10, 313-316. [ Links ]
Van Ark, H. & Meiswinkel, R., 1992, 'Subsampling of large light trap catches of Culicoides (Diptera: Ceratopogonidae)', Onderstepoort Journal of Veterinary Research 59, 183-189. [ Links ]
Venter, G.J. & Hermanides, K.G., 2006, 'Comparison of black and white light for collecting Culicoides imicola and other livestock-associated Culicoides species in South Africa', Veterinary Parasitology 142, 383-385. http://dx.doi.org/10.1016/jj.vetpar.2006.07.015 [ Links ]
Venter, G.J., Labuschagne, K., Boikanyo, S.N.B., Morey, L. & Snyman, M.G., 2011, 'The repellent effect of organic fatty acids on Culicoides midges as determined with suction light traps in South Africa', Veterinary Parasitology 181, 365-369. http://dx.doi.org/10.1016/j.vetpar.2011.04.034 [ Links ]
Venter, G.J., Labuschagne, K., Hermanides, K.G., Boikanyo, S.N.B., Majatladi, D.M. & Morey, L., 2009, 'Comparison of the efficiency of five suction light traps under field conditions in South Africa for the collection of Culicoides species', Veterinary Parasitology 166, 299-307. http://dx.doi.org/10.1016/jj.vetpar.2009.08.020 [ Links ]
Venter, G.J., Majatladi, D.M., Labuschagne, K., Boikanyo, S.N.B. & Morey, L., 2012, 'The attraction range of the Onderstepoort 220 V light trap for Culicoides biting midges as determined under South African field conditions', Veterinary Parasitology 190, 222-229. http://dx.doi.org/10.1016/jj.vetpar.2012.05.019 [ Links ]
Viennet, E., Garros, C., Lancelot, R., Allène, X., Gardès, L., Rakotoarivony, I., Crochet, D., Delécolle, J-C., Moulia, C., Baldet, T. & Balenghien, T., 2011, 'Assessment of vector/host contact: Comparison of animal-baited traps and UV-light/suction trap for collecting Culicoides biting midges (Diptera: Ceratopogonidae), vectors of Orbiviruses', Parasites and Vectors. http://dx.doi.org/10.1186/1756-3305-4-119 [ Links ]
VSN International, 2012, GenStat for Windows 15th Edition, VSN International, Hemel Hempstead, UK. [ Links ]
White, S.D. & Evans, A.G., 2002, 'Culicoides hypersensitivity', in B.P. Smith (ed.), Large animal internal medicine, pp. 1217-1218, Mosby, St. Louis. [ Links ]
Private Bag X5
Received: 24 Jan. 2013
Accepted: 17 July 2013
Published: 08 Aug. 2014