versão On-line ISSN 2224-9435
versão impressa ISSN 1019-9128
J. S. Afr. Vet. Assoc. vol.82 no.2 Pretoria Jan. 2011
CLINICAL COMMUNICATION KLINIESE MEDEDELING
L Le Roux-PullenI,*; D LessingII
IDepartment of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110 South Africa
IIFrankfort Dierekliniek, PO Box 215, Showgrounds, Frankfort, 9830 South Africa
Three clinically healthy Labrador puppies developed ataxia, hypermetria and convulsions shortly after eating the burnt crust of maize porridge. Two of the puppies died. Acrylamide toxicity was considered based on the history of all 3 puppies developing nervous signs after being exposed to a starch-based foodstuff that was subjected to high temperature during preparation. Acrylamide-induced neurotoxicity is thought to partially result from a distal axonopathy.
Keywords: acrylamide, ataxia, convulsions, distal axonopathy, high temperature, hypermetria, starch.
The presence of acrylamide, a potentially toxic substance, was only discovered in starch-rich foodstuffs as recently as 2002. The synthesis of acrylamide in starch-containing foods such as potatoes, cereals and bread is dependent on exposure to high temperatures10. The risk this compound poses to the consumer at the concentrations that occur in regularly consumed foodstuffs is still being debated, but prompted the European Chemical Agency to add acrylamide to the list of substances of very high concern in 20104.
The acrylamide monomer is a potent neurotoxin that has been known for over 50 years. Numerous toxicity trials have been conducted in mammals, including dogs.
The acrylamide monomer is a white, odourless, crystalline solid at room temperature and is used to produce non-toxic poly-acrylamide polymers. These polymers have multiple industrial uses, among others in wastewater treatment and the textile industry2.
The toxic effect and subsequent clinical manifestation depend on the degree of exposure to acrylamide. The clinical neurological signs are attributed to a distal axonopathy, otherwise known as a 'dying-back' neuropathy9,11,13.
Clinical signs reported in humans after acute acrylamide exposure include sweating, nausea, myalgia, speech disorders, numbness and weakened extremeties2.
To the authors' knowledge there are no previous cases documented of dogs that incidentally developed acrylamide neuropathy through their daily diet, but some of the trials during which acrylamide has been intentionally administered to domestic animals are discussed. The effects of acrylamide administration in dogs were found to be similar than those observed in other species12.
A Labrador bitch and her 3, 2-month-old puppies, all clinically healthy and in good physical condition, were fed maize porridge that was badly burnt. A day after exposure the owner reported that 1 of the puppies suffered a seizure and died. Soon afterwards another puppy started walking in an abnormal manner, vomited and had convulsions, but 24 to 48 hours after the 1st signs were noted, the puppy recovered. When the 3rd puppy started showing similar signs, the owner consulted a private veterinary practitioner.
On clinical examination the puppy was ataxic and displayed hypermetria. No other neurological signs were seen, although a full neurological examination was not done. The puppy was in good bodily condition. The temperature, pulse, respiratory parameters and mucous membrane colour were within normal limits. There was no oculonasal discharge or diarrhoea present. A blood smear showed no abnormalities and was negative for Babesia spp. parasites.
The veterinarian administered watersoluble vitamins (Vitamin B Co, Oberon Pharma), dexamethasone (Dexafort, Intervet Schering-Plough Animal Health) and intravenous fluid therapy. The puppy was hospitalized for observation. Despite sustained treatment the puppy's condition deteriorated. He developed tetraparesis and died 3 days later.
On post mortem examination a hard, black, thin, oval object 3 cm in diameter was found in the stomach. No other macroscopic lesions were noticed. The foreign object was identified as burnt porridge, which the owner fed to the puppy a few days earlier. The dam and litter had been fed porridge before and could have been exposed to burnt remnants on previous occasions.
All the puppies in this litter exhibited similar neurological signs in a short period , and either intoxication or the involvement of an infectious agent were considered as the most likely differential diagnoses for their ailment.
There was no history of exposure to any exogenous neurotoxins. The clinical signs that typically accompany frequently seen organic and inorganic intoxications in dogs (including methaldehyde, strychnine and tremogenic mycotoxins) were absent3. The typical haematological pathology seen in lead poisoning was not present on the blood smear. The puppies' daily diet included a good commercial puppy feed, making a thiamine deficiency unlikely. The involvement of neurotropic infectious agents causing diffuse neurological signs were ruled out as unlikely based on history and clinical examination. The following agents were excluded:
Toxocara canis: although ascarid toxaemia is a likely diagnosis in this case, none of the puppies showed any other signs of worm infestation prior to eating the burnt maize porridge, no worms were present in the puppy on which a post mortem was performed, and the dam was dewormed shortly before whelping.
Neospora caninum: neither the dam nor the puppies had any exposure to bovine placental tissue, neosporosis has never been diagnosed in cattle of the surrounding farming community, and the dam had whelped and raised normal puppies before.
Toxoplasma gondii: this parasite is more commonly seen in immunosuppressed animals and less likely in dogs, and the more commonly seen respiratory and gastrointestinal signs accompanying toxoplamosis were not observed in these puppies. The classic neurological sign of hyperextended pelvic limbs seen in young animals suffering from toxoplamosis or neosporosis were absent in all 3 puppies3.
Distemper virus: although the puppies had not yet received their 1st inoculation prior to the development of clinical signs, the dam was fully vaccinated, and the clinical signs that typically precede neurological signs in puppies infected with distemper virus after birth were not present3.
It is nevertheless a limitation of this report that no tests were done to confirm the absence of the abovementioned differential diagnoses.
Acrylamide is well absorbed by most routes, but exposure usually occurs through oral intake or contact with the skin. The monomer is distributed widely throughout the body and metabolized by the liver to non-toxic metabolites, which are mainly excreted in the urine9. Studies have demonstrated rapid and large-scale absorption in dogs after oral intake of acrylamide6. The resulting neuropathy and the severity of the syndrome that follows intoxication are dependent on the magnitude of the dose, the rate of administration and the period of exposure to the toxin11. The peripheral (motor and sensory) and central nervous systems are affected, but the latter seems to require higher doses of toxin. The neuropathy is widely accepted to be an example of a central- peripheral distal axonopathy13. This is defined as a process whereby the distal portion of the longest peripheral axons is affected 1st, but after continuous exposure the distal segments of corticospinal, spinocerebellar and dorsal column axons also become involved. Ataxia resulting from cerebellar dysfunction follows owing to afferent and efferent cerebellarand Purkinje fibre degeneration1,11. More recent studies suggest that terminal degeneration might be the primary site of pathology and precedes axonopathy8. With high-level acute acrylamide exposure, however, the clinical picture is typically an encephalopathy with accompanying ataxia and seizures, followed by a peripheral neuropathy. Widespread autonomic dysfunction can occur11. Acrylamide also has an effect on calmodulin (CaM) concentration and protein kinase C (PKC) activity, offering a hypothesis that this may be another mechanism whereby acrylamide induces neuropathy14.
Earlier studies in cats revealed severe tonic-clonic convulsions and other signs of a diffuse central nervous excitation when lethal doses (100 mg/kg adult feline) of acrylamide monomer were administered intravenously. Administration of sublethal doses induced ataxia and tremors7. When adult male Beagle dogs and miniature pigs were fed 1 mg acrylamide/kg/day in the diet for 3-4 weeks no neurotoxic signs were elicited, but acrylamide was present in muscle tissue collected at post mortem examination. Although the nervous system is the primary target for the acrylamide monomer, less than 1 % of the substance was detected in the brain. Neuropathy was observed in the dogs and pigs when the acrylamide was administered at 5 mg/kg/ day for an extended period of 30 to 60 days6. Chronic exposure in dogs leads to the typical progressive sensorimotor peripheral neuropathy, including toe cuffing and ataxia and weakness, with a unique association to megaoesophagus due to vagal nerve axonopathy12. When sublethal doses of acrylamide are discontinued, the neuropathy that developed may resolve slowly. More severe deficits like spasticity and cerebellar ataxia are likely to remain1,9.
The most prominent histological finding in acrylamide neuropathy is degeneration of peripheral nerves2. Chemical analysis of tissue samples, for example muscle, could aid in the diagnosis of acrylamide poisoning. In addition to neuropathy, carcinogenicity, mutagenicity and reproductive toxicity have also been demonstrated in rats2, 13 .
The LD50 in rats is 100-150 mg/kg13. The LD50 in cats and monkeys was determined to range between 100 and 200 mg/kg after a single dose2.
Quantitative food surveys done in the UK in recent years revealed that cereal can contain up to 57 mg/kg acrylamide and a kilogram of potatoes up to 112 mg5. Levels appear to rise as food is heated for longer periods of time. To the authors' knowledge there are no figures available for the levels of acrylamide that can be present in maize porridge exposed to high temperatures for a prolonged period.
Despite strong circumstantial evidence, it is a limitation of this report that tissue sampling for histopathology or determining tissue levels of acrylamide were not done in order to confirm the suspected acrylamide toxicity.
Porridge prepared from maize meal is a staple diet for people in South Africa and the burnt remnants are often fed to household pets. The possibility of acrylamide toxicity occurring in these animals, in particular immature animals, deserves consideration and should not be ignored when neurological cases are seen. Further studies are needed to quantify the risk acrylamide in foodstuffs poses to dogs.
1. Bradley W G 2004 Neurology in clinical practice Vol. 2: The neurological disorders (5th edn). Butterworth-Heinemann Publications, Oxford [ Links ]
2. Cloeren M 1992 Acrylamide. In Sullivan J B, Krieger G R (eds), Hazardous materials toxicology. Williams and Wilkins, Maryland: 940-944 [ Links ]
3. Ettinger S J, Feldman E C 2005 Textbook of veterinary internal medicine (6th edn), Vols 1 & 2. Elsevier Saunders, Missouri [ Links ]
4. European Chemical Agency. Online at: http://echa.europa.eu/chem_data/authoris ation_process/candidate_list_table_en.asp (accessed 28 January 2011) [ Links ]
5. Food Standards Agency. Online at: http://www.food.gov.uk/multimedia/pdfs/fsis712005.pdf (accessed 17 May 2011) [ Links ]
6. Ikeda G J, Miller E, Sapienza P P, Michel T C, Inskeep P B 1987 Comparative tissue distribution and excretion of [1-14C]acrylamide in beagle dogs and miniature pigs. Food and Chemical Toxicology 25: 871-875 [ Links ]
7. Kuperman A S 1958 Effects of acrylamide on the central nervous system of the cat. Journal of Pharmacology and Experimental Therapeutics 123: 182-192 [ Links ]
8. LoPachin R M 2002 The role of fast axonal transport in acrylamide pathophysiology: mechanism or epiphenomenon? Neuro-Toxicology 23: 253-257 [ Links ]
9. Murray L, Center of Clinical Toxicology, Nashville 1999, Acrylamide. Online at: http://www.inchem.org/documents/pims/chemical/pim652 (accessed 28 January 2011) [ Links ]
10. National Food Administration, Uppsala, Sweden 2002. Online at: http://www.mind fully.org/Food/Acrylamide-Heat-ProcessedFoods26apr02.htm (accessed 28 January 2011) [ Links ]
12. Satchell P M, McLeod J G, 1981 Megaoesophagus due to acrylamide neuropathy. Journal of Neurology, Neurosurgery and Psychiatry 44: 906-913 [ Links ]
13. Tareke E, Rydberg P, Karlsson P, Eriksson S, Tornqvist M 2002 Analysis of acrylamide, a carcinogenic formed in heated foodstuffs. Journal of Agricultural and Food Chemistry 50: 4998-5006. [ Links ]
14. YunHe Z, Ming J, Lu H 2010 Effects of acrylamide on calmodulin and protein kinase C in spinal cord of rats. Journal of Environment and Health 27: 598-600 [ Links ]
Received: March 2011.
Accepted: June 2011.