On-line version ISSN 2224-9435
J. S. Afr. Vet. Assoc. vol.81 n.2 Pretoria Jan. 2010
SHORT COMMUNICATION KORT BERIG
S MukaratirwaI,*; V P SinghII
ISchool of Biological and Conservation Sciences, University of KwaZulu-Natal, Westville Campus, Durban, 4000 South Africa
IIState Veterinary Services, Department of Agriculture, PO Box 527, Umzimkulu, 3297 South Africa
Coprological examination was used to determine the prevalence and intensity of gastrointestinal parasites of stray dogs impounded by the Society for the Prevention of Cruelty to Animals (SPCA), Durban and Coast, South Africa. Helminth and protozoan parasites were found in faeces of 240 dogs with an overall prevalence of 82.5 % (helminth parasites 93.1 % and protozoan parasites 6.9 %). The following parasites and their prevalences were detected; Ancylostoma sp. (53.8 %), Trichuris vulpis (7.9 %), Spirocerca lupi (5.4 %), Toxocara canis (7.9 %), Toxascaris leonina (0.4 %) Giardia intestinalis (5.6 %) and Isospora sp. (1.3 %). Dogs harbouring a single parasite species were more common (41.7 %) than those harbouring 2 (15 %) or multiple (2.1 %) species. Ancylostoma sp., Toxocara canis and Giardia intestinalis have zoonotic potential and were detected in 66.7 % of the samples.
Keywords: Durban, gastrointestinal parasites, intensity, prevalence, SPCA, stray dogs, zoonosis.
Gastrointestinal (GI) parasites are common pathogens in stray dogs and some are reservoirs of parasitic infections of humans, particularly in urban areas and especially in informal urban areas. Some of the important zoonotic diseases include visceral larva migrans due to Toxocara canis, cutaneous larva migrans caused by Ancylostoma caninum and A. braziliensis, giardiosis, cryptosporidiosis and echinococcosis8. Transmission may occur from dogs to humans directly or indirectly.
The prevalence of GI parasites may vary due to several factors, including geographical region, level of veterinary care before straying and habits of the animal7. In most cities in developing countries control of stray dogs is practically nonexistent, resulting in an increased risk of exposure to zoonoses transmitted by these animals19.
Several studies have demonstrated that stray dogs have a high prevalence of gastrointestinal parasites18,19. In South Africa there are reports on prevalence of GI parasites in dogs from resourcelimited communities16,17 and a metropolitan population from Pretoria20. Minaar et al.16 examined 106 dogs and found Ancylostoma spp. as the most common helminth parasite followed by T. canis.
Information on GI parasites of urban stray dogs is lacking in South Africa and the purpose of this study was to provide baseline information on GI parasite infection of stray dogs on a local scale in the Durban metropole, South Africa.
MATERIALS AND METHODS
Source of samples
In this study a stray dog is defined as any dog roaming in a public place without its owner or a person who is responsible for it and consequently impounded by the Society for the Prevention of Cruelty to Animals (SPCA). Between August 2008 and January 2009, faecal samples were collected in plastic containers from 240 male and female stray dogs of ages varying between 6 months and 6 years. The dogs had been impounded by the SPCA, Durban and Coast, Springfield Park, and were accommodated in individual kennels. The faecal samples were collected from each dog at the 1st day of impoundment. The dogs were of a variety of breeds from various suburbs in the Durban metropole, South Africa.
Fresh faecal samples were processed individually and examined microscopically for helminth eggs and protozoan cysts and/or oocysts after concentration by the modified formol-ether technique13.
Briefly, 1 g of faeces was added to 7 m of 10 % formol-saline in a 15 mℓ test tube. The content was mixed thoroughly and strained through a 200-µm aperture sieve and collected into another test tube. The suspension was centrifuged at 2000 rpm (Sigma 204, swing-out, rotor diameter 12 cm, g-force 536.6 ) for 3 min and the supernatant fluid decanted. The procedure was repeated by adding formolsaline to the sediment to make up 12 mℓ and centrifuged and the supernatant decanted. Eight mℓ of buffered-alcohol (H2PO4) solution was added to the sediment and the solution stirred using a wooden applicator to break up the sediment followed by addition of 4 mℓ of ether. The solution was vigorously shaken and centrifuged at 1500 rpm for 1 min, and the supernatant fluid decanted. The remaining sediment was diluted with 3mℓ of normal saline and strained through a 200 µ aperture sieve into a clean 15 mℓ centrifuge tube. The filtrate was left to settle for 15 min and the supernatant fluid decanted. The sediment was transferred to a glass slide using a Pasteur pipette and the tube was rinsed with a few drops of saline to remove the last bits of sediment and examined under a light microscope. The number of eggs/cysts/ oocysts were identified18,24 , counted and expressed as eggs/cysts/oocysts per gram of faeces.
Faecal samples were recorded as positive if 1 egg/cyst/oocyst was observed in the faecal analysis. The prevalence of infection for each parasite was calculated as the number of positive samples divided by the total number of samples tested expressed as a percentage . Intensity of infection was quantified by the number of eggs/cysts/oocysts for each parasite in a gram of faeces. The infection status of each dog was classified into either no infection, single or multiple infection.
The prevalence of GI parasites in stray dogs is shown in Table 1 and indicates that 82.5 % of the dogs were infected by 8 genera and/or species. Ancylostoma sp. had the highest prevalence (53.8 %) followed by T. canis and Trichuris vulpis (both 7.9 %) and the lowest was Toxascaris leonina and Taenia sp. (both 0.4 %). Species of zoonotic potential represented by Ancylostoma sp., T. canis and Giardia sp. were detected in 66.7 % of the samples (Table 2).
The observed infection status of GI parasites and the intensity of infection are shown in Tables 1 and 2, respectively. Intensities of 50-500 eggs/cysyts/oocysts per gram of faeces were recorded in 26.4 % of the dogs with Ancylostoma sp.infected dogs contributing the highest percentage (16.7 %). A small proportion of the dogs (6.7 %) had more than 5000 eggs/cysts/oocysts per gram of faeces with Ancylostoma sp.-infected dogs again contributing the highest percentage (3.7 %). No dogs infected with T. leonina, Giardia intestinalis or Isospora sp. had an intensity of infection >5000.
Infection with 1 species of parasite was more common (41.7 %) than double infection (15 %) and multiple infection (2.1 %) (Table 2). A single infection of Ancylostoma sp., T. vulpis, T. canis and Giardia intestinalis was recorded in 84 (35 %), 6 (2.5 %), 5 (2.1 %) and 5 (2.1 %) dogs respectively (Table 2).
Helminth parasites were more prevalent (6 species/groups) than protozoans (2 species/group). A large proportion of dogs had a low to medium intensity of infection by helminth parasites while the protozoan parasites had an evenly distributed intensity of infection and no dogs were recorded with intensity of infection >5000 oocysts/cysts per gram of faeces.
The overall prevalence of GIT parasites in stray dogs found in this study revealed a very high level of infection comparable with studies elsewhere18. Helminth infection predominated over protozoan infections in this study and this is similar to other studies14. The history of deworming of the dogs in our study was unknown and they might have been a mixture of dogs which were routinely dewormed before straying and those that were not dewormed. However, the increased helminth infection and reduced protozoan infection is most likely due to decreased routine use of anthelmintics in the stray dogs in contrast to what has been observed in other studies3.
Ancylostoma sp. was the most prevalent parasite species and this is similar to findings from other studies16,18,20,27. The species of Ancylostoma are the most pathogenic in dogs and are also involved in human infection as the cause of cutaneous larva migrans11. The high prevalence suggests that the environmental conditions in Durban are conducive for the survival and transmission of the parasite, but reports on human infection are nonexistent. There is a possibility that Ancylostoma sp. might have developed resistance to the common anthelmintics16 as some dogs from this study must have been regularly dewormed before straying.
Prevalence of T. canis observed in this study is comparable with that reported in Zimbabwe18 but lower than what has been reported in dogs from resource-poor communities in South Africa16. The difference might have been due to the differences in the age distribution of dogs sampled as young dogs that acquire infection via congenital transmission tend to have a high prevalence compared with adults24.
Trichuris vulpis is one of the common intestinal helminiths in dogs in Europe1,5,6,21. The prevalence of 7.9% from this study fell within the wide range of values that have been reported by the above authors. Surprisingly, the parasite was absent in the previous studies in South Africa16,17but reported in stray dogs in neighbouring Zimbabwe18. Human infections by T. vulpis have been reported9,12,25 and have been attributed to humans being in continuous contact with environments contaminated by infected dogs.
The prevalence of S. lupi in dogs in some parts of South Africa has been reported and in urban stray dogs in Zimbabwe15,17,18 with rates comparable to our study. However, the prevalence rate recorded from our study is on the lower side compared with what has been reported elsewhere2,4,10. Variation in prevalence of this parasite has been mainly attributed to high prevalence of intermediate and paratenic hosts and whether the dogs are stray, farm dogs or pets26.
Giardia intestinalis was the most prevalent protozoan recorded in this study, similar to what has been reported in other studies3,11,22. In a similar study in Zimbabwe18, Giardia intestinalis was second in prevalence to Cryptosporidium sp., a protozoan which was surprisingly absent from our study despite the use of a sensitive technique3. High prevalence of Giardia in dogs in Australia has been attributed to the fact that most of the anthelmintics do not interfere in the development of the parasite and the ability to colonise niches previously occupied by parasites such as T. canis and Dipylidium caninum3.This does not seem to be the case for the recorded prevalence of Giardia intestinalis as the parasite was also recorded in dogs with multiple infections involving helminth parasites.
Isospora sp. was recovered with a prevalence of 1.3 % and has been reported as an important cause of diarrhoea, especially in puppies23.
The results from this study serve as baseline information on the potential risk posed by stray dogs to urban environmental contamination by zoonotic parasites.
Special appreciation to the management and staff of the Society for the Prevention of Cruelty to Animals (SPCA), Durban and Coast, for their cooperation and assistance in collecting the faecal specimens.
1. Barutzki D, Schaper R 2003 Endoparasites in dogs and cats in Germany 1999-2002. Parasitology Research 90: 148-150 [ Links ]
2. Brodey R S, Thompson R G, Sayer P D, Eugster B 1977 Spirocerca lupi infection in dogs in Kenya. Veterinary Parasitology 3: 49-59 [ Links ]
3. Bugg R J, Robertson I D, Elliot A D, Thompson R C 1999 Gastrointestinal parasites of urban dogs in Perth, Western Australia. Veterinary Journal 157: 295-301 [ Links ]
4. Chandrasekharon K P, Sastry G A, Menon M N 1958 Canine spirocercosis with special reference to the incidence and lesions. British Veterinary Journal 114: 388-395 [ Links ]
5. Fok E, Szatmari V, Busak K, Rozgonyi F 2001 Prevalence of intestinal parasites in dogs in some urban and rural areas of Hungary. Veterinary Quarterly 23: 96-98 [ Links ]
6. Georgieva D, Ivanov A, Prelesov P 1999 Studies on the parasitic fauna in stray dogs in the Stara Zagora region. Bulgarian Journal of Veterinary Medicine 2: 121-124 [ Links ]
7. Jittapalapong S, Inparnkaew T, Pinyopanuwat N, Kengradomkij C, Sangvaranond A, Wongnakphet S 2007 Gastrointestinal parasites of stray cats in Bangkok Metropolitan areas, Thailand. Katsetsart Journal of Natural Science 41: 69-73 [ Links ]
8. Kaewthamasorn K, Niwetpathomwat A, Assrasakaron S, Wongsamee S, Tiawsirisup S 2006 A surveillance of canine gastrointestinal parasites fecal samples from public areas of Bangkok, Thailand. Journal of Animal and Veterinary Advances 5: 1209-1213 [ Links ]
9. Kagei N, Hayashi S, Kato K 1986 Human cases of infection with canine whipworms, Trichuris vulpis (Froelich, 1789), in Japan. Japanese Journal of Medical Science and Biology 39: 177-184 [ Links ]
10. Kamara J A 1964 The incidence of canine spirocercosis in the Freetown area of Sierra Leone. Bulletin of Epizoology Diseases of Africa 12: 465-469 [ Links ]
11. Katagiri S, Oliveira-Sequeira T C G 2008 Prevalence of dog intestinal parasites and risk perception of zoonotic infection by dog owners in Saõ Paulo State, Brazil. Zoonoses Public Health 55: 406-413 [ Links ]
12. Kenney M, Yermakov V 1980 Infection of man with Trichuris vulpis, the whipworm of dogs. American Journal of Tropical Medicine and Hygiene 29: 1205-1208 [ Links ]
13. Knight W B, Hiatt R A, Cline B L, Ritchie L S 1976 A modification of the formol-ether concentration technique for increased sensitivity in detecting Schistosoma mansoni eggs. American Journal of Tropical Medicine and Hygiene 25: 818-823 [ Links ]
14. Labruna M B, Pena H F G, Souza S L P, Pinter A, Silva J C R, Ragozo A M A, Camargo L M A, Gennari S M 2006 Prevalence of endoparasites in dogs from the urban area of Montenegro municipality, Rondônia. Arquivos do Instituto Biológico (São Paulo) 73: 183-193 [ Links ]
15. Lobetti R 2000. Survey of the incidence, diagnosis, clinical manifestations and treatment of Spirocerca lupi in South Africa. Journal of the South African Veterinary Association 71: 43-46 [ Links ]
16. Minaar, W N, Krecek R C, Rajput R J 1999 Helminth parasites of dogs from two resource-limited communities in South Africa. Journal of South African Veterinary Association 70: 92-94 [ Links ]
17. Minaar W N, Krecek R C, Fourie L J 2002 Helminths in dogs from a peri-urban resource-limited community in Free State Province, South Africa. Veterinary Parasitology 107: 343-349 [ Links ]
18. Mukaratirwa S, Busayi R 1995 A survey of patent gastrointestinal parasites of stray dogs in Bulawayo urban area. Zimbabwe Veterinary Journal 26: 19-27 [ Links ]
19. Oliveira-Sequiera T C, Amarane A F, Ferrari T B, Nunes L C 2002 Prevalence of intestinal parasites in dogs from São Paulo State Brazil. Veterinary Parasitology 103: 19-27 [ Links ]
20. Ortlepp R J 1934 Echinococcus in dogs from Pretoria and vicinity. Onderstepoort Journal of Veterinary Science and Animal Industry 3: 97-108 [ Links ]
21. Overgaauw P A M, Boersema J H 1998 Nematode infections in dog breeding kennels in the Netherlands, with special reference to Toxocara. Veterinary Quarterly 20: 11-14 [ Links ]
22. Palmer C S, ThompsonRCA,Traub R J, Res R, Robertson I D 2008 National study of the gastrointestinal parasites of dogs and cats in Australia. Veterinary Parasitology 151: 181-190 [ Links ]
23. Ramírez-Barrios R A, Barboza-Mena G, Munoz J, Angulo-Cubillan F, Hernandez E, Gonzalez F, Escalona F 2004 Prevalence of intestinal parasites in dogs under veterinary care in Maracaibo, Venezuela. Veterinary Parasitology 121: 11-20 [ Links ]
24. Reinecke R K 1983. Veterinary helminthology. Butterworth, Durban [ Links ]
25. Singh S, Samantaray C, Singh N, Das G B, Verma I C 1993 Trichuris vulpis infection in Indian tribal population. Journal of Parasitology 79: 457-45 [ Links ]
26. Van der Merwe L L, Kirberger R M, Clift S, Williams M, Keller N, Naidoo V 2008 Spirocerca lupi infection in the dog: a review. Veterinary Journal 176: 284-309 [ Links ]
27. Verster A 1979 Gastro-intestinal helminths of domestic dogs in the Republic of South Africa. Onderstepoort Journal of Veterinary Research 46: 79-82 [ Links ]
Received: November 2009.
Accepted: April 2010.