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Journal of the South African Veterinary Association

On-line version ISSN 2224-9435

J. S. Afr. Vet. Assoc. vol.80 n.4 Cape Town  2009

 

ARTICLE ARTIKEL

 

Detection of the bovine viral diarrhoea/mucosal disease (BVD/MD) virus in tissues from aborted ruminant foetuses using immunohistochemistry

 

 

S M NjiroI; C M NkosiII

IOnderstepoort Veterinary Institute, Private Bag X5, Onderstepoort, 0110 South Africa, and Diagnosis of Zoonotic Diseases, Food, Feeds and Veterinary Public Health, Onderstepoort Veterinary Institute
IIDepartment of Anatomical Pathology, PO Box 213, MEDUNSA, 0204 South Africa

 

 


ABSTRACT

Various tissues from aborted ruminant foetuses were collected, fixed in formalin and embedded in paraffin wax. Sections were made and exposed to a primary monoclonal antibody against the bovine viral diarrhoea/mucosal disease (BVD/MD) virus, and subsequently to a goat anti-mouse secondary antibody conjugated to horse radish peroxidase (HRP). Diaminobenzidine (DAB) was the substrate and it released a brown pigment in the tissues on reacting with the HRP in an immunohistochemistry (IHC) procedure. Of 27 aborted foetuses, an immunoperoxidase staining reaction was observed in 1 ovine and 5 bovine foetuses. The IHC procedure located BVD/MD viral antigen in a wide variety of foetal tissues including cerebral cortical neurons, the pseudostratified columnar epithelial cells lining the bronchi, alveolar lining cells and alveolar macrophages, hepatocytes, renal tubular lining cells and the Purkinje fibres in the myocardium.

Keywords: aborted ruminant foetuses, BVD/MD virus, IHC


 

 

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Received: May 2009
Accepted: October 2009

 

 

* Author for correspondence. E-mail: njiros@arc.agric.za

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