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Journal of the South African Veterinary Association

versão On-line ISSN 2224-9435
versão impressa ISSN 1019-9128

J. S. Afr. Vet. Assoc. vol.80 no.3 Cape Town  2009

 

ARTICLE

 

Efficacy of a genotype 2 Newcastle disease vaccine (Avinew®) against challenge with highly virulent genotypes 5d and 3d

 

 

D G BwalaI, II; C AbolnikIII; A van WykI; E CorneliusIV; S P R BisschopI

IPoultry Reference Centre, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110 South Africa
IINational Veterinary Research Institute, pmb 01, Vom 930010, Nigeria
IIIBiotechnology Department, Onderstepoort Veterinary Institute, Private Bag X05, Onderstepoort, 0110 South Africa
IVDepartment of Veterinary Tropical Diseases, Faculty of Veterinary Science, Private Bag X04, Onderstepoort, 0110 South Africa

 

 


ABSTRACT

Since 2002, following its introduction, the lineage 5d Newcastle disease virus (so-called Goose paramyxovirus - GPMV) strain has caused numerous disease outbreaks among commercial and backyard poultry in South Africa, raising questions about the ability of commercially available Newcastle disease vaccines to fully protect poultry against the strain. This study aimed to determine whether there are differences in the level of protection offered by Avinew® Newcastle disease vaccine against GPMV virus as compared with a 3d Newcastle disease virus isolated in South Africa in 1993 (Rainbow challenge virus - RCV) strain. Six groups of 10-day-old, specific pathogen-free chickens were vaccinated with doses of 103.0, 104.5 and 106.0 EID50 of Avinew® vaccine and challenged at 4 weeks of age intramuscularly at a dose of 105.3 EID50/ 0.2 mi/bird of GPMV and RCV No statistically significant difference could be found in the protection offered by Avinew® vaccine against GPMV as compared to RCV challenge. The protection offered against the ND challenge was found to be dose dependent. At the recommended field dose of 106.0 EID50 the vaccine gave 100 % protection from mortality against both the challenge viruses, but not against infection and replication of the viruses, as gross lesions were evident even in apparently healthy birds that survived the challenge. The protective dose (PD90) of the Avinew® vaccine against GPMV challenge was calculated at 104.38 and against that of RCV at 104.43.

Keywords: lineage 3d, lineage 5d (GPMV), Newcastle disease, vaccine


 

 

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Received: June 2009
Accepted: August 2009

 

 

*Author for correspondence. E-mail: shahn.bisschop@up.ac.za

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