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Journal of the South African Veterinary Association

On-line version ISSN 2224-9435
Print version ISSN 1019-9128

J. S. Afr. Vet. Assoc. vol.79 n.4 Cape Town  2008

 

ARTICLE ARTIKEL

 

Provisional clinical chemistry parameters in the African Sharptooth catfish (Clarias gariepinus)

 

 

J G MyburghI; C J BothaI; D G BooyseII; F ReyersIII

IDepartment of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110 South Africa
IIDepartment of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110 South Africa
IIIDigital Veterinary Diagnostics, PO Box 41468, Garsfontein East, 0060 South Africa

 

 


ABSTRACT

Pollution affects aquatic systems worldwide and there is an urgent need for efficient monitoring. Fish are generally sensitive to their environment and are thus considered to be valuable bioindicator species. The African Sharptooth catfish (Clarias gariepinus)is particularly important in this respect because of its very wide distribution. In order to use C. gariepinus as a bioindicator species its baseline clinical chemistry must be defined. Existing data are scarce, and the objective of this work was therefore to establish clinical chemistry parameters for C. gariepinus. Blood was collected from male and female catfish and a number of clinical chemistry parameters were determined. Plasma protein values, but particularly those of plasma albumin, were found to be very low, approximately half the value for dogs, but similar to the values in Channel catfish (Ictalurus punctatus). Plasma urea values in Sharptooth catfish were found to be much lower than in dogs, but only marginally lower than in Channel catfish. Plasma creatinine in Sharptooth catfish, however, was only a quarter of that of dogs and one third of that found in Channel catfish. These findings may have implications for using urea and/or creatinine as an index of renal glomerular filtration, as is done in mammals. Plasma enzyme activity ranges were much lower in Sharptooth catfish than in dogs, particularly for alkaline phosphatase (ALP) and alanine amino-transferase (ALT). By comparison, Channel catfish have an even lower ALT activity range but an ALP range that is very similar to dogs. The implications for using these enzymes as markers for liver disease are not clear from these data, as factors such as plasma half-life and tissue distribution remain tobe determined. The very lowplasma thyroxine (T4) levels have important implications for laboratory personnel, who will have to set up calibration and standardisation adaptations for the methods that are generally designed for human samples. Although the sample size was too small for reliable comparisons, it appeared that there was little difference in the parameters measured between male and female fish. The values obtained are a useful startingpoint for using C. gariepinus as abioindicator species.

Key words: bioindicator, Clarias gariepinus, clinical chemistry, plasma enzymes, proteins, renal function, Sharptooth catfish, thyroxine, T4


 

 

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REFERENCES

1. Barnhoorn I E, Bornman M S, Pieterse G M 1999 Histological evidence of intersex in feral sharptooth catfish (Clarias gariepinus) from an estrogen-polluted water source in Gauteng, South Africa. Environmental Toxicology 19: 603-608        [ Links ]

2. Colborn T, Dumanoski D, Myers J P 1996 Our Stolen Future. Dutton Books, New York        [ Links ]

3. Colborn T, Thayer K 2000 Aquatic ecosystems: harbinger of endocrine disruption. Ecological Applications 10: 949-957        [ Links ]

4. Ellsaesser C F, Clem L W 1987 Blood serum chemistry measurements of normal and acutely stressed catfish. Comparative Biochemistry & Physiology. Part A: Comparative Physiology 88: 589-594        [ Links ]

5. Evans D H, Claiborne J B 2006 Physiology of fishes. CRC Press Taylor & Francis Group, Boca Raton, Florida        [ Links ]

6. Gupta B B P, Premabati Y 2002 Differential effects of melatonin on plasma levels of thyroxine and triiodothyronine levels in the air-breathing fish, Clarias gariepinus, during breeding and quiescent periods. General and Comparative Endocrinology 129: 146-151        [ Links ]

7. Heath R G M, Claassen M 1999 An overview of the pesticide and metal levels present in populations of the larger indigenous fish species of selected South African rivers. Water Research Commission Report, #428/1/99        [ Links ]

8. Horn P S, Feng L, Li Y, Pesce A J 2001 Effect of outliers and nonhealthy individuals on reference interval estimation. Clinical Chemistry 47: 2137-2145        [ Links ]

9. Horn P S, Pesce A J 2003 Reference intervals: an update. Clinica Chimica Acta 334: 5-23        [ Links ]

10. Ip Y K, Subaidah R M, Liew P C, Loong A M, Hiong K C, Wong W P, Chew S F 2004 African Sharptooth catfish Clarias gariepinus does not detoxify ammonia to urea or amino acids but actively excretes ammonia during exposure to environmental ammonia. Physiological and Biochemical Zoology 77: 242-254        [ Links ]

11. Latimer K S, Mahaffey E A, Prasse K W 2003 Duncan and Prasses's veterinary laboratory medicine (4th edn). Iowa State University Press        [ Links ]

12. Rodriguez-Cea A, Linde Arias A R, Fernandez de la Campa M R, Costa Moreira J, Sanz-Mendel A 2006 Metal speciation of metallothionein in white sea catfish, Netuma barba and pearl cichlid, Geophagus brasiliensis, by orthogonal liquid chromatography coupled to ICP-MS detection. Talanta 69: 963-969        [ Links ]

13. Rumke C L, Bezemer P D 1972 Method for the determination of normal values. II. New method. Nederlands Tijdschrift voor Geneeskunde 116: 1559-1568        [ Links ]

14. Siroka Z, Drastichova D 2004 Biochemical markers of aquatic environment contamination-Cytochrome P450 in fish. A review. Acta Veterinaria Brno 73: 123-132        [ Links ]

15. Skelton P 2001 A complete guide to the freshwater fishes of southern Africa. Southern Book Publishers, Halfway House        [ Links ]

16. Solberg E H, Lahti A 2005 Detection of outliers in reference distributions: performance of Horn's algorithm. Clinical Chemistry 51: 2326-2332        [ Links ]

17. Travlos G S, Morris R W, Elwell M R, Duke A, Rosenblum S, Thompson M B 1996 Frequency and relationships of clinical chemistry and liver and kidney histopathology findings in 13-week toxicity studies in rats. Toxicology 107: 17-29        [ Links ]

18. Van der Oost R, Beyer J, Vermeulen N P E 2003 Fish bioaccumulation and biomarkers in environmental riskassessment: a review. Environmental Toxicology and Pharmacology 13: 57-149        [ Links ]

19.Van Vuren J H J, Van der Merwe M, Du Preez H H 1994 The effect of copper on the blood chemistry of Clarias gariepinus (Clariidae). Ecotoxicology and Environmental Safety 29: 187-199        [ Links ]

 

 

Received: March 2007
Accepted: November 2008

 

 

* Author for correspondence. E-mail: jan.myburgh@up.ac.za

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