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Journal of the South African Veterinary Association

versão On-line ISSN 2224-9435

J. S. Afr. Vet. Assoc. vol.79 no.3 Cape Town  2008

 

ARTICLE ARTIKEL

 

Gammaherpesvirus carrier status of black wildebeest (Connochaetes gnou) in South Africa

 

 

J A Pretorius; M C Oosthuizen; M van Vuuren

Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110 South Africa

 

 


ABSTRACT

Malignant catarrhal fever (MCF) is an economically important disease primarily of domestic cattle with a high case fatality rate. It is causedby either alcelaphine herpesvirus type 1 (AlHV-1) or ovine herpesvirus type 2 (OvHV-2). The major reservoir host of AlHV-1 is the blue wildebeest (Connochaetes taurinus),but it is generally accepted that the black wildebeest (Connochaetes gnou) is also a reservoir host. No viral studies in the black wildebeest have been reported and the carrier status of black wildebeest has not been documented. Specimens were collected from several game farms and conservation areas in central South Africa representing the geographical area historically linked to the natural habitat of the black wildebeest. Specimens were obtained from 304 black wildebeest of different ages and sex, as well as 51 black wildebeest foetuses at different stages of gestation. Virus was isolated from a black wildebeest calf. Morphological features and antigenic characteristics suggested it to be a gammaherpesvirus closely related to AlHV-1. All serum samples tested positive with a competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA) for group-specific malignant catarrhal fever virus antibody. A SYBR Green real-time PCR assay was developed for the detection of gammaherpesviral DNA. Only 15.8 % of the animals tested positive with the real-time PCR assay whereas 90 % of the foetuses tested positive. This finding suggests that, unlike OvHV-2 infection in lambs in which the infection takes place after weaning, the virus in black wildebeest is mainly transmitted in utero or soon after birth. The results suggest that black wildebeest are latent carriers of a gammaherpesvirus similar or closely related to AlHV-1 present in blue wildebeest and that it is likely that all black wildebeest are persistently infected.

Key words: alcelaphine herpesvirus type 1, black wildebeest, Connochaetes gnou, gamma-herpesvirus, malignant catarrhal fever


 

 

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REFERENCES

1. Barnard B J H 1990 Epizootiology of wildebeest-derived malignant catarrhal fever: Possible transmission among cows and their calves in the north-western Transvaal. Onderstepoort Journal of Veterinary Research 57: 201-204        [ Links ]

2. Barnard B J H 1991 Malignant catarrhal fever (snotsiekte) and the role of the black wildebeest (Connochaetes gnou). Pelea 10: 43-44, 61-63        [ Links ]

3. Dungu B, Bosman A-M, Kachelhoffer C, Viljoen G J 2002 Single-tube nested PCR for detection of the sheep-associated agent of MCF. Veterinary Record 151: 703-706        [ Links ]

4. Ensser A, Pflanz R, Fleckenstein B 1997 Primary structure of the alcelaphine herpesvirus 1 genome. Journal of Virology 71: 6517-6525        [ Links ]

5. Flach E J, Reid H W, Pow I, Klempt A 2002 Gammaherpesvirus carrier status of captive artiodactyls. Research in Veterinary Science 73: 93-99        [ Links ]

6. Fraser S J, Nettleton P F, Dutia B M, Haig D M, Russell G C 2006 Development of an enzyme-linked immunosorbent assay for the detection of antibodies against malignant catarrhal fever viruses in cattle serum. Veterinary Microbiology 116: 21-28        [ Links ]

7. Handley J A, Sargan D R, Herring A J, Reid H W 1995 Identification of a region of the alcelaphine herpesvirus-1 genome associated with virulence for rabbits. Veterinary Microbiology 47: 167-181        [ Links ]

8. Keel M K, Patterson J G, Noon T H, Bradley G A, Collins J K 2003 Caprine herpesvirus-2 in association with naturally occurring malignant catarrhal fever in captive sika deer (Cervus Nippon). Journal of Veterinary Diagnostic Investigation 15: 179-183        [ Links ]

9. Li H, Dyer N, Keller J, Crawford T B 2000 Newly recognized herpesvirus causing malignant catarrhal fever in white-tailed deer (Odocoileus virgianus). Journal of Clinical Microbiology 38: 1313-1318        [ Links ]

10. Li H, Gailbreath K, Flach E J, Taus N S, Cooley J, Keller J, Russell G C, Knowles D P, Haig D M, Oaks J L, Traul D L, Crawford T B 2005 A novel subgroup of rhadinoviruses in ruminants. Journal of General Virology 86: 3021-3026        [ Links ]

11. Li H, Keller J, Knowles D P Taus N S, Oaks J L, Crawford T B 2005 Transmission of caprine herpesvirus 2 in domestic goats. Veterinary Microbiology 107: 23-29        [ Links ]

12. Li H, Shen D T, Jessup D A, Knowles D P, Gorham J R, Thorne T, O'Toole D, Crawford T B 1996 Prevalence of antibody to malignant catarrhal fever virus in wild and domestic ruminants by competitive-inhibition ELISA. Journal of Wildlife Diseases 32: 437-43        [ Links ]

13. Li H, Shen D T, Knowles D P Gorham J R, Crawford T B 1994 Competitive inhibition enzyme-linked immunosorbent assay for antibody in sheep and other ruminants to a conserved epitope of malignant catarrhal fever virus. Journal of Clinical Microbiology 32: 1674-1679        [ Links ]

14. L0ken T, Aleksandersen M, Reid H, Pow I 1998 Malignant catarrhal fever caused by ovine herpesvirus-2 in pigs in Norway. Veterinary Record 143: 464-467        [ Links ]

15. World Organisation for Animal Health 2004 Manual of diagnostic tests and vaccines for terrestrial animals (5th edn), Chapter 2.3.14: Malignant catarrhal fever. Online at: www.oie.int/engnormes/mmanual/a_00065.htm        [ Links ]

16. Michel A L, Aspeling I A 1994 Evidence of persistent malignant catarrhal fever infection in a cow obtained by nucleic acid hybridisation. Journal of the South African Veterinary Association 65: 26-27        [ Links ]

17. Mushi E Z, Karstad L, Jessett D M 1980 Isolation of bovine malignant catarrhal fever virus from the ocular and nasal secretions of wildebeest calves. Research in Veterinary Science 29: 168-171        [ Links ]

18. Peters I R, Helps C R, Hall E J, Day M J 2004 Real-time RT-PCR: considerations for efficient and sensitive assay design. Journal of Immunological Methods 286: 2038-217.         [ Links ]

19. Plowright W 1986 Malignant catarrhal fever. Revue scientifique et technique, Office International des Épizooties 5: 897-918        [ Links ]

20. Reid H W, Van Vuuren M 2004 Malignant catarrhal fever. In CoetzerJAW,Tustin R C (eds) Infectious diseases of livestock Vol. 2. Oxford University Press, Cape Town: 895-907        [ Links ]

21. Rossiter P B 1981 Immunofluorescence and immunoperoxidase techniques for detecting antibodies to malignant catarrhal fever in infected cattle. Tropical Animal Health Production 13: 189-192        [ Links ]

22. Rossiter P B, Jessett D M 1980 A complement fixation test for antigens of and antibodies to malignant catarrhal fever virus. Research in Veterinary Science 28: 228-233        [ Links ]

23. Sentsui H, Nishimori T, Nagai I, Nishioka N 1996 Detection of sheep-associated malignant catarrhal fever virus antibodies by complement fixation tests. Journal of Veterinary Medical Science 58: 1-5        [ Links ]

24. Traul D L, Elias S, Taus N S, Herrmann L M, Oaks J L, Li H 2005 A real-time PCR assay for measuring alcelaphine herpesvirus-1 DNA. Journal of Virological Methods 129: 186-190        [ Links ]

25. Ulrich M P, Norwood D A, Christensen D R, Ulrich R L. 2006 Using real-time PCR to specifically detect Burkholderia mallei. Journal of Medical Microbiology 55, 551-559        [ Links ]

 

 

Received: February 2008
Accepted: September 2008

 

 

* Author for correspondence. E-mail: jana@thabamanzi.co.za

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