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Journal of the South African Veterinary Association

On-line version ISSN 2224-9435
Print version ISSN 1019-9128

J. S. Afr. Vet. Assoc. vol.79 n.3 Pretoria  2008




The use of the milk ring test and rose bengal test in brucellosis control and eradication in Nigeria



S I B CadmusI; H K AdesokanI; J StackII

IDepartment of Veterinary Public Health and Preventive Medicine, University of Ibadan, Ibadan, Nigeria
IIDepartment of Statutory & Exotic Bacteria, Veterinary Laboratories Agency, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom




In this study, milk and blood samples collected simultaneously from 532 trade cows to be slaughtered at Bodija abattoir, Ibadan (southwestern, Nigeria) were examined for antibodies to Brucella using the milk ring test (MRT) and the rose bengal test (RBT). Overall, 18.61 % of the milk samples were positive according to the MRT, while 9.77 % of the serum samples were positive according to the RBT. The difference was highly significant (Chi-square value 16.33; P < 0.05); only 32 (6.02 %) of the samples were positive for both tests. The Red Bororo breed of cattle and the White Fulani had the highest positive rates, namely 20.93 % and 11.69 % for the MRT and RBT respectively. No conclusion can be drawn about sensitivity because we do not know the true status of the animals tested. It is, however, obvious that although the MRT and RBT are 1st-line screening tests for brucellosis in cows in some countries, their lack of specificity is of concern. Therefore, the requirement for other confirmatory tests that are more specific should be considered for control and eradication of the disease, especially in Nigeria.

Key words: brucellosis, cows, diagnosis, serology, zoonosis



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1. Acha P N, Szyfres B 1987 Zoonoses and communicable diseases common to man and animals (2nd edn). Scientific Publication No. 503, Pan American Health Organization/ World Health Organization, Washington DC        [ Links ]

2. Alton G G, Jones L M, Angus R D, Verger J M 1988 Techniques for the brucellosis laboratory. Institut National de la Recherche Agronomique, Paris        [ Links ]

3. Beh K J 1974 Quantitative distribution of Brucella antibody amongst immunoglobulin classes in vaccinated and infected cattle. Research in Veterinary Science 17:        [ Links ]

4. Beh K J 1973 Distribution of Brucella antibody among immunoglobulin classes and a low molecular weight antibody fraction in serum and whey of cattle. Research in Veterinary Science 14: 381-384        [ Links ]

5. Bercovich Z, Moerman A 1979 Non-specific positive milk ring test(s) in tank milk and Estrumater in the treatment of cattle. Tijdschrift voor Diergeneeskunde 104: 713-716        [ Links ]

6. Cadmus S I B, Ijagbone I F, Oputa H E, Adesokan H K, Stack J A 2006 Serological survey of brucellosis in livestock animals and workers in Ibadan, southwestern Nigeria. African Journal of Biomedical Research 9: 163-168        [ Links ]

7. Food and Agriculture Organisatio of the United Nations 2004 Bovine brucellosis. Animal health/disease cards. Online at: (accessed on 11 February 2008)        [ Links ]

8. Hill W K W 1966 Een onderzoek over de waarde van de abortus bang-ringtest bij melkmonsters uit melktanks. Tijdschrift voor Diergeneeskunde 14: 885-895        [ Links ]

9. Keer W R, Pearson J K L, Rankin J E F 1959 The bovine udder and its agglutinins. British Veterinary Journal 115: 105-109        [ Links ]

10. MacMillan A 1990 Conventional serological tests. Animal brucellosis 206: 153-197        [ Links ]

11. Morgan W J B 1967 The serological diagnosis of bovine brucellosis. Veterinary Record 80: 612-621        [ Links ]

12. Patterson J M, Deyoe B L 1976 Effect of physical properties of milk fat globules on Brucella ring test sensitivity. Journal of Dairy Science 60: 851-856        [ Links ]

13. Tanwani S K, Pathak P N 1971 Studies on abortus bang ring test: factors affecting the nature of reaction in different milk samples. Indian Journal for Animal Science 41: 1037-1040        [ Links ]



Received: March 2008
Accepted: July 2008



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