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Journal of the South African Veterinary Association

On-line version ISSN 2224-9435
Print version ISSN 1019-9128

J. S. Afr. Vet. Assoc. vol.79 n.1 Cape Town  2008

 

ARTICLE ARTIKEL

 

A comparison of the oral application and injection routes using the Onderstepoort Biological Products Fowl Typhoid vaccine, its safety, efficacy and duration of protection in commercial laying hens

 

 

C PurchaseI; J PicardII; R McDonaldIV; S P R BisschopIII

IDepartment of Zoology and Entomology, University of Pretoria, Pretoria, 0002 South Africa
IIDepartment of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110 South Africa
IIIPoultry Reference Centre, Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110 South Africa
IVOnderstepoort Biological Products, Private Bag X01, Onderstepoort, 0110 South Africa

 

 


ABSTRACT

This study was undertaken to establish whether the Onderstepoort Biological Products Fowl Typhoid (OBPft) vaccine registered as an injectable vaccine was effective and safe when administered orally to commercial layers. Its efficacy and duration of protection were compared with application by intramuscular injection. Commercial brown layer hens were used as they were found to be highly susceptible to Salmonella gallinarum infections. In the vaccine safety trial birds were euthanased at timed intervals spanning 4 weeks postvaccination. Necropsies were performed and samples were taken and tested. No clinical signs or mortalities could be attributed to the OBPft vaccine nor could active shedding of the vaccine strain be detected. Slight pathological changes were noted with both routes of vaccination; however, these changes were transient, returning to normal within the observation period. The injected groups showed a better serological response with the rapid serum plate agglutination (RSPA) test than the orally vaccinated groups. In the duration of protection trial, birds were challenged at 3-8-week intervals post-vaccination. All unvaccinated birds died. Protection 8 and 16 weeks after vaccination was above 60 %, by 24 weeks after challenge, the vaccine protection was below 30 %. It was found that there was no significant difference (P < 0.05) in the protection offered by either the oral or injected route of vaccination with the OBPft vaccine.

Key words: efficacy, OBP fowl typhoid vaccine, safety, Salmonella gallinarum


 

 

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REFERENCES

1. AssokuRKG,Penhale W J 1974 The pathogenesis of fowl typhoid: the influence of anaemia and erythrocyte clearance on the susceptibility of the fowl to bacterial endotoxin. Journal of Comparative Pathology 84: 443-453        [ Links ]

2. Audisio M C, Terzolo H R 2002 Virulence analysis of a Salmonella gallinarum strain by oral inoculation of 20-day-old chickens. Avian Diseases 46: 186-191        [ Links ]

3. Barrow P A 1993 Salmonella control- past, present and future. Avian Pathology 22: 651-669        [ Links ]

4. Barrow P A, Berchieri Jr. A, Al-Haddad O 1992 Serological response of chickens to infection with Salmonella gallinarum-S. pullorum detected by enzyme-linked immuno-sorbent assay. Avian Diseases 36: 227-36        [ Links ]

5. Berchieri Jr. A, De Oliveira G H, Pinheiro L A S, Barrow P A 2000 Experimental Salmonella gallinarum infection in light laying hens. Brazilian Journal of Microbiology 31: 50-52        [ Links ]

6. Bouzoubaa K, Nagaraja K V, Newman J A, Pomeroy B S 1987 Use of membrane proteins from Salmonella gallinarum for prevention of fowl typhoid infection in chickens. Avian Diseases 31: 699-704        [ Links ]

7. Calnek B W, Barnes H J, Beard C W, Reid W M,Yoder H W 1991 Diseases of poultry (9th edn). Iowa State Press, Ames        [ Links ]

8. Calnek B W, Barnes H J, Beard C W, Saif Y M 1997 Diseases of poultry (10th edn). Iowa State Press, Ames        [ Links ]

9. Cameron C M, Buys S B 1979 Production and application of a live Salmonella gallinarum vaccine. Onderstepoort Journal of Veterinary Research 46: 185-189        [ Links ]

10. Harbourne J F 1957 The control of fowl typhoid in the field by the use of live vaccines. Veterinary Record 1102-1107        [ Links ]

11. Intervet 2001 Intervet product catalogue 36-37        [ Links ]

12. Jordan T W, Pattison M 1996 Poultry diseases, (4th edn). Iowa State Press, Ames        [ Links ]

13. Saif Y M, Barnes H J, Glisson J R, Fadly A M, McDougald L R, Swayne D E 2003 Diseases of poultry (11th edn). Iowa State Press, Ames        [ Links ]

14. Silva E N, Snoeyenbos G H, Weinack O M, Smyser C F 1981a Studies on the use of 9R strain of Salmonella gallinarum as a vaccine in chickens. Avian Diseases 25: 38-52        [ Links ]

15. Silva E N, Snoeyenbos G H, Weinack O M, Smyser C F 1981b The influence of native gut microflora on the colonization and infection of Salmonella gallinarum in chickens. Avian Diseases 25: 68-73        [ Links ]

16. Young J, Lee I P M, Kang M S 2005 Safety and efficacy of Salmonella Gallinarum 9R vaccine in young laying chickens. Avian Pathology 34: 362-366        [ Links ]

 

 

Received: July 2007
Accepted: March 2008

 

 

* Author for correspondence: E-mail: cpurchase@zoology.up.ac.za

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