SciELO - Scientific Electronic Library Online

 
vol.61 índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Articulo

Indicadores

Links relacionados

  • En proceso de indezaciónCitado por Google
  • En proceso de indezaciónSimilares en Google

Compartir


South African Journal of Chemistry

versión On-line ISSN 1996-840X
versión impresa ISSN 0379-4350

S.Afr.j.chem. (Online) vol.61  Durban  2008

 

RESEARCH ARTICLE

 

Critical self-assembly concentration of bolaamphiphilic peptides and peptide hybrids determined by fluorescence measurements

 

 

Marco Martari *, **; Ronald D. Sanderson

Department of Chemistry and Polymer Science, Stellenbosch University, Private Bag X1, Matieland, 7602 South Africa

 

 


ABSTRACT

The study of the self-assembly properties of peptides and proteins is important for the understanding of molecular recognition processes and for the rational design of functionalbiomaterials. Novel bolaamphiphilic peptides and peptide hybrids incorporating non-natural aminoacids were designed around a model lysine/leucine-rich peptide with the intention to study their self-assembly behaviour. Steady-state fluorescence measurements using pyrene as fluorescent probe were adapted to the determination of the critical self-assembly concentrations (CSACs) of these amphiphilic peptides and peptide hybrids. Different experimental conditions were studied. The morphology of the peptide aggregates was evaluated by scanning electron microscopy (SEM). Concentration and pH have been revealed to play a key role in the control of the process. Peptides presented different three-dimensional supramolecular arrangements that were correlated with their aminoacid compositions (specifically considering the presence of tyrosine and proline) and CSAC values.

Keywords: Peptide aggregation, self-assembly, critical self-assembly concentration, fluorescence, pyrene


 

 

Full text available only in pdf format.

 

Acknowledgements

M.M. would like to thank Dr M. Rautenbach (Department of Biochemistry, Stellenbosch University) and Dr J. Juodaityte (Department of Chemistry and Polymer Science, Stellenbosch University) for helpful discussions.

 

References

1 K. Rajagopal and J.P. Schneider, Curr. Opin. Struct. Biol., 2004, 14, 480-486.         [ Links ]

2 S. Zhang and M. Altman, React. Funct. Polym., 1999, 41, 91-102.         [ Links ]

3 S. Zhang, Nature Biotechnol., 2003, 21, 1171-1178.         [ Links ]

4 A. Aggeli, M. Bell, L.M. Carrick, C.W.G. Fishwich, R. Harding, P.J. Mawer, S.E. Radford, A.E. Strong and N. Boden, J. Am. Chem. Soc., 2003, 125, 9619-9628.         [ Links ]

5 R.C. Claussen, B.M. Rabatic and S.I. Stupp, J. Am. Chem. Soc., 2003, 125, 12680-12681.         [ Links ]

6 K.L. Niece, J.D. Hartgerink, J.J.J.M. Donners and S.I. Stupp, J. Am. Chem. Soc., 2003, 125, 7146-7147.         [ Links ]

7 H. Matsui and G.E. Douberly, Langmuir, 2001, 17, 7918-7922.         [ Links ]

8 J.H. Collier, B.H. Hu, J.W. Ruberti, J. Zhang, P. Shum, D.H. Thompson and P.B. Messersmith, J. Am. Chem. Soc., 2001, 123, 9463-9464.         [ Links ]

9 D.J. Pochan, J.P. Schneider, J. Kretsinger, B. Ozbas, K. Rajagopal and L.B. Haines, J. Am. Chem. Soc., 2003, 125, 11802-11803.         [ Links ]

10 J.H. Fuhrhop and J. Mathieu, Angew. Chem., Int. Ed. Engl., 1984, 23, 100-113.         [ Links ]

11 K. Yamada and S. Natori, Biochem. J., 1994, 298, 623-628.         [ Links ]

12 J. Alvarez-Bravo, S. Kurata and S. Natori, Biochem. J., 1994, 302, 535-538.         [ Links ]

13 VB. Naidoo, Supramolecular Structures of Biomacromolecular Assemblies, Ph.D. thesis, University of Stellenbosch, Stellenbosch, South Africa, 2004.         [ Links ]

14 M. Martari, Structure-Function Relationships of Bolaamphiphilic Peptides and Peptide Hybrids, Ph.D. thesis, University of Stellenbosch, Stellenbosch, South Africa, 2006.         [ Links ]

15 P. Chen, Colloids Surf. A, 2005, 261, 3-24.         [ Links ]

16 H. LeVine III, Protein Sci., 1993, 2, 404-410.         [ Links ]

17 M.R. Nilsson, Methods, 2004, 34, 151-160.         [ Links ]

18 R. Ninomiya, K. Matsuoka and Y. Moroi, Biochim. Biophys. Acta, 2003, 1634, 116-125.         [ Links ]

19 M.S. Bakshi and G.J. Kaur, J. Colloid Interface Sci, 2005, 289, 551-559.         [ Links ]

20 D. Yin, W. Yang, Z. Ge and Y. Yuan, Carbohydr. Res., 2005, 340, 1201-1206.         [ Links ]

21 S. Zhang, T. Holmes, C. Locksmith and A. Rich, Proc. Natl. Acad. Sci. U.S.A., 1993, 90, 3334-3338.         [ Links ]

22 S.Y. Fung, C. Keyes, J. Duhamel and P. Chen, Biophys. J., 2003, 85, 537-548.         [ Links ]

23 M.R. Caplan, P.N. Moore, S. Zhang, R.D. Kamm and D.A. Lauffenburger, Biomacromolecules, 2000, 1, 627-631.         [ Links ]

24 M.R. Caplan, E. Schwartzfarb, S. Zhang, R.D. Kamm and D.A. Lauffenburger, Biomaterials, 2002, 23, 219-227.         [ Links ]

25 E. Gazit, FASEB J., 2002, 16, 77-83.         [ Links ]

26 K. Wang, J.D. Keasling and S.J. Muller, Int. J. Biol. Macromol., 2005, 36, 232-240.         [ Links ]

27 S. Vauthey, S. Santoso, H. Gong, N. Watson and S. Zhang, Proc. Natl. Acad. Sci. U.S.A., 2002, 99, 5535-5360.         [ Links ]

28 P. Messina, M.A. Morini, P.C. Schulz and G. Ferrat, Colloid Polym. Sci., 2002, 280, 328-335.         [ Links ]

29 E. Atherton, M.J. Gait, R.C. Sheppard and B.J. Williams, Bioorg. Chem., 1979, 8, 351-370.         [ Links ]

30 E. Atherton and R.C. Sheppard, Solid-phase Synthesis: A Practical Approach (D. Rickwood and B.D. Hames, eds.), 1st edn., Oxford University Press, Oxford, UK, 1989.         [ Links ]

31 L.A. Carpino and G.Y. Han, J. Am. Chem. Soc., 1970, 92, 5748-5749.         [ Links ]

32 E. Kaiser, R.L. Colescott, C.D. Bossinger and P.I. Cook, Anal. Biochem., 1970, 34, 595-598.         [ Links ]

33 T. Christensen, Acta Chem. Scand., 1979, 33B, 763-766.         [ Links ]

34 Y. Fang and F. Cheng, Proceedings of the 2006 AlChE Annual Meeting, Sessionl62ag, San Francisco, CA, USA, 12-17 November 2006, American Institute of Chemical Engineers, New York, NY, USA, 2006.         [ Links ]

 

 

Received 6 November 2007
Revised 20 February 2008
Accepted 20 February 2008

 

 

* To whom correspondence should be addressed. E-mail: marco.martari@gmail.com.
** Present address: Johns Hopkins University, School of Medicine, Baltimore, MD, USA.

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons