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South African Journal of Chemistry
On-line version ISSN 1996-840X
Print version ISSN 0379-4350
S.Afr.j.chem. (Online) vol.61 Durban 2008
RESEARCH ARTICLE
Critical self-assembly concentration of bolaamphiphilic peptides and peptide hybrids determined by fluorescence measurements
Marco Martari *, **; Ronald D. Sanderson
Department of Chemistry and Polymer Science, Stellenbosch University, Private Bag X1, Matieland, 7602 South Africa
ABSTRACT
The study of the self-assembly properties of peptides and proteins is important for the understanding of molecular recognition processes and for the rational design of functionalbiomaterials. Novel bolaamphiphilic peptides and peptide hybrids incorporating non-natural aminoacids were designed around a model lysine/leucine-rich peptide with the intention to study their self-assembly behaviour. Steady-state fluorescence measurements using pyrene as fluorescent probe were adapted to the determination of the critical self-assembly concentrations (CSACs) of these amphiphilic peptides and peptide hybrids. Different experimental conditions were studied. The morphology of the peptide aggregates was evaluated by scanning electron microscopy (SEM). Concentration and pH have been revealed to play a key role in the control of the process. Peptides presented different three-dimensional supramolecular arrangements that were correlated with their aminoacid compositions (specifically considering the presence of tyrosine and proline) and CSAC values.
Keywords: Peptide aggregation, self-assembly, critical self-assembly concentration, fluorescence, pyrene
Full text available only in pdf format.
Acknowledgements
M.M. would like to thank Dr M. Rautenbach (Department of Biochemistry, Stellenbosch University) and Dr J. Juodaityte (Department of Chemistry and Polymer Science, Stellenbosch University) for helpful discussions.
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Received 6 November 2007
Revised 20 February 2008
Accepted 20 February 2008
* To whom correspondence should be addressed. E-mail: marco.martari@gmail.com.
** Present address: Johns Hopkins University, School of Medicine, Baltimore, MD, USA.