versión impresa ISSN 0375-1589
S. Afr. j. anim. sci. vol.40 no.3 Pretoria 2010
B.D. NkosiI,#; R. MeeskeII
IARC-LBD: Animal Production Institute, P/Bag x 2, Irene 0062, South Africa
IIWestern Cape Department of Agriculture, P.O. Box 249, George 6530, South Africa
The aim of the study was to determine the effect of whey or molasses on the fermentation quality when added to potato hash silage. In addition, lamb performance, digestibility and feed intake of diets containing potato hash silage were compared with a diet containing maize silage (MSd). Potato hash silage (treated with no additive, or whey, or molasses) and MS were produced in 210 L drums for 90 days and the fermentation quality of the silages was determined thereafter. Diets were formulated and fed ad libitum to 32 South African Dorper lambs (23.5 ± 0.873 kg live weight) for 63 days. A digestibility study was conducted during the last week of the study. The untreated potato hash silage (UPHS) was poorly fermented as indicated by higher concentrations of butyric acid, ammonia-N and pH compared to the other silages. Higher dry matter intake (DMI) and daily weight gains (218 and 250 g/d) were obtained with MSd and molasses treated potato hash silage diet (MPHSd) compared to the other diets. Nutrient digestibility was lower in the UPHS diet compared to the other dietary treatments. It was concluded that the fermentation quality of potato hash was improved with the addition of whey and molasses. Furthermore, growth performance was higher with the MSd and MPHSd than of the other treatments, suggesting that MPHSd can safely replace MSd in a lamb diet at a dietary inclusion level of 20% without any adverse effect on animal performance.
Keywords: Digestibility, dry matter, fermentation, intake, maize silage
A major constraint to profitable livestock production under resource poor farmers in the Gauteng Province (South Africa) is the limited feed supply throughout the year (GADS, 2006). This province is experiencing a rapid growth in human population coupled with high demands for housing which limits availability of land suitable for fodder production. The utilization of less traditional feeds such as byproducts combined with roughage sources may provide farmers with a variety of feeding options. By-product feeds are produced by a number of food processing industries, and such resources may impact traditional ruminant feeding practices by reducing the amount of concentrates fed to ruminants, providing feeding options when there is a scarcity of feed and reducing feed cost. Potato hash, a by-product from the production of potato snacks and chips, is available in large amounts in the Gauteng Province and is not widely used in livestock feeding. This by-product contains 150 g dry matter (DM)/kg of fresh potato hash, 700 g starch/kg DM, 11.16 MJ metabolisable energy (ME)/kg DM, 105 g crude protein (CP)/kg DM, 370 g neutral detergent fibre (NDF)/kg DM and 163 g acid detergent fibre (ADF)/kg DM (Nkosi, 2009). If it is not consumed, it often gets mouldy and sour, and therefore unlikely to be used as an animal feed. Because of problems associated with the fresh form of potato hash, drying and ensiling are two options of preserving high moisture by-products. However, the drying process is costly and may not be affordable to the resourcepoor farmers.
Interest in conserving by-products by ensiling is steadily increasing, largely due to the increase in their use as animal feeds (Megias et al., 1998; Kayouli & Lee, 1999; Bakshi et al., 2006; Kholif et al., 2007). Properly ensiled silage from high moisture by-products can replace costly feeds such as maize silage in ruminant diets (Itavo et al., 2000; Lallo et al., 2003; Pirmohammadi et al., 2006). However, ensiling of potato hash requires substantial amounts of fermentable sugars to produce sufficient lactic acid to reduce the pH and stabilize the product (McDonald, 1981; Wilkinson, 2005). Generally, potato by-products contain relatively low concentrations of water soluble carbohydrates (WSC) and lactic acid bacteria (LAB) (O`Kiely et al., 2002; Okine, 2007) the latter being killed off during the food processing of the potato (Moon, 1981). Thus, potato by-products require silage additives to improve the fermentation during ensiling. Some food byproducts have been ensiled successfully with chemical additives resulting in an improvement in fermentation quality and digestibility of the silage (Megias et al., 1998; Kholif et al., 2007).
However, chemical additives have their own limitations since they are corrosive to the equipment used and can be dangerous to handle. Consequently, biological additives are often preferred (Gwayumba, 1997). Biological additives have also their own limitations. They are costly to the farmer and their effectiveness can be less reliable, since it is based on the activity of living organisms (Weinberg & Muck, 1996). Alternatively, food waste materials such as whey (Nkosi, 2003; Zobell et al., 2004; Bautista-Trujillo et al., 2009) and sugarcane molasses (Bolsen et al., 1996; Yunus et al., 2000; Van Niekerk et al., 2007; Nkosi et al., 2009) can be used as silage additives to ensile high moisture potato hash. The present study compared the fermentation characteristics of potato hash silage produced without or with additives (whey and molasses) with that of maize silage. This was followed by a study on the feed intake and performance of lambs consuming the silage diet and the digestibility of the diets.
Materials and Methods
Potato hash was collected from Simba (PTY) LTD (Andre Greyvensteyn Avenue, Isando, South Africa), a food producing factory in the Gauteng Province, and brought to the Agricultural Research Council -Irene Institute (longitude 28º13`S : latitude 25º55`E, altitude 1524 m) South Africa for chemical analysis, silage making and the lamb feeding trial. To prevent effluent loss during ensiling, potato hash silage was prepared by mixing 800 g potato hash/kg (850 g H2O/kg and /kg DM: 105 g CP, 43 g ash, 370 g aNDF, 163 g ADF and 110 g ether extract (EE)) with 200 g/kg of Eragrostis curvula hay (60 g H2O/kg and /kg DM: 45 g CP, 39 g ash, 789 g aNDF, 432 g ADF and 33 g EE). The silage-hay mixture was subjected to the following treatments: untreated control (no additive), or additives as whey or molasses added to the silage mixture. Where molasses was used, it was diluted with warm water at a ratio of 1 : 2 (4 h before application), and was sprayed over the material at a application rate of 30 L per ton fresh material (FM). Whey was screened for lactic acid bacteria (LAB) concentration, and contained a population of 6.95 x 105 LAB cfu/mL (± 0.341 s.e.m.) before ensiling. Whey was sprayed at 30 L per ton FM to obtain at least >1.7 x 104 cfu/g FM. Maize silage, a fourth silage treatment was produced by chopping whole crop maize (Senkuil, Sensako, Brits, South Africa) (360 g H2O/kg, a pH of 5.7 and 43 g WSC/kg DM) with a Feraboli 945 precision silage chopper (Fondata Nel, Cremona, Italy) to obtain a 5 mm chop length, and was ensiled in 210 L drums without an additive. In order to compensate, water that was added to the treated silage; both the maize and untreated potato hash were sprayed with 30 L of distilled water over a ton of FM to keep them at the same level of moisture as the treated silages. The materials were ensiled in 210 L drums, lined with plastic bags and closed with rubber lids to prevent damages to the bags by rodents. After 90 d of ensiling, the drums were opened and samples were collected and analysed for chemical composition and fermentation characteristics.
Experimental diets that contained either the potato hash silages or maize silage were formulated as shown in Table 1. The dietary treatments were: a) maize silage diet (MSd), b) untreated potato hash silage diet (UPHSd), c) whey treated potato hash silage diet (WPHSd) and d) molasses treated potato hash silage diet (MPHSd). Samples of the diets were collected fortnightly to determine the chemical composition. The diets were fed ad libitum to 32 South African Dorper lambs (23.5 ± 0.873 kg live weight) housed in individual metabolism crates (2.2 m2) in an insulated well-ventilated barn. The lambs were allocated in a randomized complete block design on the basis of live weight to the four diets, resulting in eight lambs per treatment. Lambs were ear tagged, and treated against internal and external parasites before the commencement of the trial. Feed intake was measured daily while live weight was measured at the start of the trial and at weekly intervals until the end of the trial. A 14 day dietary adaptation period was offered and the trial lasted for 63 days. All animals were treated according to the regulations of the Animal Ethics Committee of the ARC-API (2008).
A seven-day faecal collection period was conducted a week after the growth study. Lambs were fitted with leather harnesses and canvass bags attached to the back of each lamb three days before the collection period started. Daily feed intake was recorded and faeces were collected. Faeces accumulated for the seven day period were pooled per lamb and sub-samples were collected for laboratory analyses. Faeces samples were frozen at -20 ºC.
A 40 g silage sample from each drum was collected and mixed with 360 mL of distilled water in a stomacher bag, homogenized and left at 10 ºC for 24 h (Suzuki & Lund, 1980). It was then homogenized for 4 min and filtered through Whatman No. 4 filter paper (G.I.C. Scientific, Midrand, South Africa). The extract was used for determination of pH, WSC, volatile fatty acids (VFAs), lactic acid (LA) and ammonia-N (NH3-N). The WSC were determined by the phenol-sulphuric acid method according to Dubois et al. (1956) and lactic acid, using the colorimetric method of Barker & Summerson (1941) as modified by Pryce (1969). The VFA concentrations were determined with a Varian 3300 FID Detector gas chromatograph (Varian Associates, Inc., Palo Alto, CA, USA) by the procedure of Suzuki & Lund (1980). Ammonia-N was determined by distillation using a Buchi 342 apparatus and a Metrohm 655 Dosimat with an E526 titrator according to AOAC (1990). This method is based on the method of Pearson & Muslemuddin (1968) for determining volatile nitrogen.
The DM of silage and the diets was determined by drying the samples at 60 ºC in a forced air oven until a constant mass was achieved (AOAC, 1990). After drying, the samples were ground through a 1 mm screen (Wiley mill, Standard Model 3, Arthur H. Thomas Co., Philadelphia, PA) for chemical analyses. Following the procedures of Van Soest et al. (1991), the aNDF concentration was determined using amylase (Sigma-Aldrich Co. LTD., Gillingham, UK, no. A-1278) and 2-ethoxyethanol, and the ADF concentration was determined using the Fibretec System equipment (Tecator LTD., Thornbury, Bristol, UK). Residual ash content was taken into account for both aNDF and ADF results. Crude protein, ash and EE were determined according to the procedure of AOAC (1990), while ME was determined, using the gas production technique of Pienaar (1994).
Data for the fermentation and chemical composition of the silage and diets was subjected to ANOVA for randomized complete design, while that of growth and digestibility studies were subjected to a randomized complete block design using Genstat (2000). Where F value was significant (P <0.05), statistical differences between means were declared using the Fisher's protected least significance difference (LSD).
Results and Discussion
Data on the chemical composition and fermentation characteristics of the silages are presented in Table 2. After 90 days of ensiling, the DM of MS was higher (P <0.05) than that of the potato hash silages due to low DM content (250 g/kg) of the latter at pre-ensiling. Water-soluble carbohydrates are regarded as essential substrates for the growth of LAB for proper fermentation, and low levels may restrict LAB growth (McDonald et al., 1991). Haigh & Parker (1985) suggested that a concentration of 30 g WSC/kg DM in a herbage is critical for successful fermentation. The concentration of WSC in the potato hash mixture at preensiling was 22 g/kg DM, indicating that it was not enough for efficient fermentation. Although potato hash contains high levels of starch, starches are only sparingly soluble in water and lactic acid bacteria do not have the ability to ferment starch (McDonald et al., 1991). This justified the addition of whey and molasses to improve the fermentation process.
The pH of potato hash silages after 90 days of ensiling was reduced to 4.5 for the UPHS and 4.2 for the MPHS and WPHS. However, the pH of UPHS was not low enough for efficient preservation. Weissbach (1968) stated that for efficient preservation of material with DM contents of 200 and 250 g/kg, a pH of 4.20 and 4.35, respectively, are required. According to McDonald et al. (2002) silage with a pH range of 3.8 to 4.2 is considered well preserved and the UPHS did not reach this target. Moreover, good quality silage is characterized by a lactic acid concentration of 30 - 140 g/kg (Zobell et al., 2004) and the UPHS silage had a lactic acid concentration (26 g/kg DM) lower than this level. Maize silage had the lowest (P <0.05) pH, acetic acid, butyric acid, propionic acid and NH3-N, and higher lactic acid (P <0.05) concentrations compared to the potato hash silages. It has been reported that silage from maize can be produced without the use of additives due to the fact that maize has a low buffering capacity and enough sugar for efficient fermentation (McDonald, 1981; Meeske, 2005). This might be the reasons for its better fermentation quality compared to the potato hash silages.
The fermentation characteristics of MS recorded in the present study correspond well with that of maize silage with a DM content of 300 to 400 g/kg, reported by Kung & Shaver (2001). This study further revealed that the addition of whey and molasses increased (P <0.05) the concentration of lactic acid, reduced silage pH and the concentrations of butyric acid and NH3-N compared to the UPHS, indicative of wellpreserved silages (McDonald et al., 1991). This result agrees well with previous work that reported higher lactic acid concentrations, lower pH and NH3-N concentration when molasses (Bolsen et al., 1996; Yunus et al., 2000) and whey (Zobell et al., 2004; Bautista-Trujillo et al., 2009,) were added to a forage at ensiling compared to the control. Moreover, whey and molasses addition reduced (P <0.05) the fibre content of the silage as compared to MS and UPHS. This could be attributed to partial hydrolysis of hemicelluloses in the treated silages (Muck & Kung, 1997). Our result agrees with Fazaeli et al. (2003) and Guney et al. (2007) who reported a decrease in fibre content of liquid whey treated straw silage and for molasses treated sorghum silage, respectively.
Ammonia-N in silage reflects the degree of protein degradation (Wilkinson, 2005), and well-preserved silages contain less than 100 g NH3-N/kg total nitrogen (TN) (McDonald et al., 2002). The silages in the present study had NH3-N concentrations of less than 100 g NH3-N/kg TN. However, treating potato hash silage at ensiling with either whey or molasses further reduced (P <0.05) the NH3-N concentration compared to the UPHS, confirming the work of other researchers (Yunus et al., 2000; Bautista-Trujillo et al., 2009; Nkosi et al., 2009) who found reduced NH3-N production. This could be explained by the fact that whey and molasses reduced the pH, resulting in a decreased production of NH3-N in the silage compared to the UPHS. The higher concentration of NH3-N in UPHS led to a decrease in the CP content of the silage compared to the other silages.
A higher (P <0.05) concentration of butyric acid occurred in the UPHS, leading to a reduced energy content of the silage compared to the other silages. It is well established that adding molasses and whey reduced the concentration of butyric acid in silage (Bautista-Trujillo et al., 2009; Nkosi et al., 2009). A concentration of <0.1 g butyric acid/kg DM is typically found in well-preserved silage (Kung & Shaver, 2001) and only the MS had the acceptable butyric acid concentration compared to the potato hash silage. Butyric acid is associated with a clostridial type of fermentation and is usually associated with high moisture silages (McDonald, 1981), and MS had a higher DM content compared to the potato hash silages. The ME content in the MPHS, WPHS and MS were within the range of 9.6 - 12.2 MJ ME/kg DM, typically reported for silages (Wilkinson, 2005). The reduction of ME in the UPHS might be attributed to the high butyric acid content, which is an indication of the loss of energy in the silage (McDonald, 1981).
According to Wilkinson (2005) silages should contain 72 - 89 g CP/kg DM (McDonald, 1981) and 9.6 - 12.2 MJ ME/kg DM and would require supplementation to achieve a daily gain of more than 150 g/d in commercial lamb operations (Marley et al., 2007). Feeding lambs on silage alone generally leads to either loss of live weight or limited daily gains (Fitzgerald, 1986). Speijers et al. (2005) obtained daily gains of 45 g/d from lambs that were fed lucerne silage supplemented with 250 g sugarbeet pellets. Data on the chemical composition of the silages (Table 2) showed that they were low in DM, CP and energy content. Therefore diets that contained <200 g/kg silage (either potato hash or maize silages) were formulated (Table 1) to improve the nutritive value of the silage diets. The chemical composition of the diets (Table 3) shows that the diets had similar (P >0.05) energy levels, but had a different (P <0.05) content of DM, CP, fibre, EE and in vitro organic matter digestibility (IVOMD). The maize silage diet contained higher (P <0.05) concentrations of the fibre fractions while the fibre fractions for WPHSd and MPHSd were lower.
Data on the growth performance and nutrient digestibility in lambs fed the experimental diets are shown in Table 4. Lambs fed the MS and MPHS diets had higher (P <0.05) dry matter intake (DMI), ADG and final live weights compared to those in the other diets. This might be attributed to a higher DM and CP content in the two silages which are known to improve DMI and growth rates in ruminants (Mustafa et al., 2008). Moreover, feeding sheep on maize silage is known to result in a positive associative effect on feed intake (Provenza, 1995). It has been reported that finishing lambs on a diet that contained 180 g apple pomace silage/kg depressed DMI compared to a dried apple pomace diet (Karami et al., 1996). In contrast, Jetana et al. (2009) did not report a depressed DMI when a diet containing 200 g pineapple wastes silage/kg was fed to buffaloes. The present study recorded DMI of 1056, 1099, 1188 and 1250 g/d for the UPHS, WPHS, MPHS and the MS diets, respectively. Taasoli & Kafilzadeh (2008) recorded DMI of 938 g/d in lambs fed a diet that contained 300 g apple pomace silage/kg, which is lower than those of the present study.
The present study recorded daily gains of 192, 205, 218 and 250 g/d for UPHSd, WPHSd, MSd and MPHSd, respectively. Rams fed on a halophytic silage without supplementation recorded gains of 162 g/d (Abdul-Aziz et al., 2001) which is lower than those of the present study. However, the work of Taasoli & Kafilzadeh (2008) reported daily gains of 199.8 g/d, comparable to those of the present study. Meeske & Basson (1998) recorded daily gains of 255 g/d in lambs fed a ration that contained 600 g maize silage/kg DM of diet, which is comparable to the MSd of the present study. However, Bosman et al. (2000) obtained a growth rate of 154 g/d in lambs fed a diet that contained 700 g/kg maize silage which is lower than that of MS diet in the present study. Higher (P <0.05) final weights were obtained in the MSd and MPHSd.
Lambs with higher feed conversion rates (FCR) require longer finishing periods and a FCR of <5 indicates a relatively good feeding management with the diet (Bosman et al., 2000). Better (P <0.05) FCR was obtained with MPHSd and WPHS (4.8 and 5.2) compared to 5.7 from the other diets. Dietary addition of 300 g apple pomace silage/kg resulted in a FCR of 4.69 in lambs (Taasoli & Kafilzadeh, 2008) comparable to the MPHSd in the present study. Higher FCR (9 - 12) were recorded in Omani lambs fed ration that contained 60% forage (Mahgoub et al., 2000). Other workers reported lower values (5.7 to 4.1) but these were obtained with rations of higher concentrate inclusion rates (Pineda et al., 1998; Archimede et al., 2007) which are in agreement with the results of the present study.
The digestibility of DM and OM was similar (P >0.05) for the MS, MPHS and WPHS diets, but higher (P <0.05) than that of UPHSd. The MS and MPHS diets had higher (P <0.05) digestibility of CP compared to the other diets which led to improved growth performance in lambs compared to those fed on other diets. In addition, MS diet had the higher (P <0.05) IVOMD and digestibility of ADF and aNDF compared to the other diets.
It can be concluded that whey and molasses addition improved the fermentation characteristics of potato hash silage. Improved lamb performance was obtained with MPHSd followed by MSd, suggesting that MPHSd can replace MSd in lamb diet at 20% dietary inclusion level without any adverse effect on animal performance. Furthermore, molasses addition to potato hash at ensiling improved its acceptability and nutrient digestibility in lambs compared to the control.
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