RESEARCH IN ACTION

 

Struvite infection calculi in dogs: problems with urinary calculus identification, and the value of the results

 

 

John Dunlevey^{I, *}; Michael Laing^II

^ISchool of Earth Science, University of KwaZulu-Natal, Durban 4041, South Africa
^IISchool of Pure and Applied Chemistry, University of KwaZulu-Natal, Durban

 

 

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ABSTRACT

Identifying the crystalline components of a urinary calculus is a valuable aid to correct diagnosis, and subsequent treatment of the underlying cause of the problem. Calculus formation is often an end product brought about by urine with a high concentration of insoluble material causing bladder irritation. This promotes bacterial infection by Proteus, which not only raises the pH in the bladder and generates ammonia, but also provides sites for mineral nucleation and crystal growth. Treating the infection and removing the stone will not necessarily cure the disorder. Correct identification of a calculus as struvite and even its potassium analogue can be critical in choice of curative protocols, but this is not always straightforward. Our experience with 'problem calculi' is described.

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Introduction

We have been systematically analysing urinary calculi by the X-ray powder diffraction method since 1978, in both humans and domestic animals.^1–3 The formation of a struvite calculus is normally due to the presence of ammonia and a raised pH in the urine, caused by infection. We have found over the past four years that some struvite bladder calculi from dogs display abnormal intensity peak patterns that are not immediately identifiable, even through the d-spacings of the planes appear to be generally correct.⁴ These discrepancies are so large as to be of significance and warrant reporting.

 

Experimental

X-ray powder diffraction (XRD) patterns were recorded with cobalt K_α radiation (λ = 1.7889 Å) as described in ref. 1. A selection of observed patterns is shown in Fig. A (see supplementary material online), and a list of the d-spacings and the relative intensities of diffraction peaks are given in Table 1 (online). The calculi were also analysed for the presence of various chemical elements using the EDAX technique on a JEOL scanning electron microscope, because the presence of calcium is indicative of amorphous hydroxyl apatite, which often exists as an amorphous solid and is therefore not evident in the XRD scan.

 

Results

Typical analytical data are presented in Fig. B (online). The results from a collection of struvite stones are given in Fig. 1 and Fig. A (online). A comparison of the observed peak intensities (Table 1) with the standard pattern⁴ shows quite clearly that few of the samples (e.g. A–D, Table 1) give the 'normal' intensity distribution. For convenience, the diffraction patterns in Table 1 are grouped by the common most intense diffraction peak, whicharises from the 4.26, 5.60, 2.69 and 4.13 Å planes. The powder diffraction patterns in Fig. A illustrate the problem, and it is hard to believe that all were generated from the same crystalline compound. Thus, there will be a major problem if one uses the set of four strongest lines as the primary criterion (Hanawaldt search) for identifying the 'unknown'. However, there are always the same groups of d-spacings with strong reflections {5.90, 5.60, 5.38}; {4.26, 4.13}; {2.80, 2.69, 2.66}, but the strongest may occur in any of the three groups (compare the examples in Table 1: Suzy 5.60 Å [G]; Dallas 4.26 Å [D];Cleo 2.80 Å [S] and Cheeky 2.69 Å [U]).

 

 

The problem of potassium

The most commonly occurring mineral species in calculi from dogs is struvite, an ammonium magnesium phosphate hydrate [NH₄MgPO₄·6H₂O]. However, we were surprised when potassium was detected by EDAX in several of the stones. It transpires that a well-defined potassium analogue [KMgPO₄·6H₂O] of struvite exists, where K⁺ replaces the NH₄⁺ ammonium cations. These two compounds are isostructural,^6,7 with the existence of a complete isomorphous series from 100% K⁺ to 100% NH₄⁺ struvite.⁶ This ion replacement is possible, as the ionic radii of K⁺ and NH₄⁺ are almost identical⁸ (1.52 vs 1.51 Å).

It is evident that when the struvite (infection) calculus forms and is growing, any potassium present in the urine can be incorporated into the crystal structure of the stone. This potassium can be detected by EDAX, but can also show itself by subtle effect on all the d-spacings (those of ideal pure struvite are about 2% greater than those of the potassium analogue). Table 2 (see supplementary material online) compares the unit cell dimensions and important d-spacings of the two crystalline compounds. It is clear that material that contains both K⁺ and NH₄⁺ cations will have d-spacings of intermediate size; but the effect is very small, with differences being detectable only in the second decimal place of the d-spacing. Unfortunately, this level of precision is not usually attainable during typical routine powder diffraction analysis of calculi samples.

Figures C and D (both online) are projections of the crystal structures along the b-axis. It is evident that the site occupied by the K⁺ and NH₄⁺ ions lies on the 110, 020, 021, 200 and 130 planes and diffraction from these should be most affected by the replacement of NH₄⁺ by K⁺ (Table 2).

An unusual result

Calcium is regularly found in calculi,^2,3 most commonly as the phosphate [hydroxyl apatite Ca₅(PO₄)₃(OH)] and less often as an oxalate [monohydrate whewellite CaC₂O₄·H₂O; dihydrate weddelite CaC₂O₄·2H₂O]. The struvite calculi from the dog named Rambo consist of two types of material: (i) dark, hard, containing only magnesium (typical struvite); (ii) softer, granular containing some calcium. The intensities of the diffraction peaks from the type (ii) material were distinctly weaker than those from the type (i) material, indicating that type (ii) was less crystalline, that is, the internal structure was not as regular as in the ideal struvite structure. Close analysis of the XRD powder pattern reveals a broad background hump between 35° and 39° 2θ (d-spacing between 2.72 and 2.81 Å) and indicates the presence of amorphous hydroxyl apatite. The presence of apatite in a calculus is indicative of hyperparathyroidism. ^1,2

 

Discussion

The anomalous intensity patterns are immediately obvious and can arise from several sources. Examples are: orientation effects from abnormal crystal shape due to the mode of crystal growth;¹⁰ particle size effects; degree of pressure during grinding of the sample; loss of water from the crystal structure; isomorphous replacement of some magnesium by calcium, or ammonium by potassium. Our results show that there is no direct correlation between calcium content and the magnitude or pattern of anomalies in the X-ray powder patterns of the struvite in these stones. Where calcium is detected by EDAX or XRF, it is usually associated with solid, amorphous, hydroxyl apatite.

The results show that there are two types of complementary information obtainable. X-Ray powder diffraction immediately identifies the calculus as a 'struvite infection' stone, and thus the patient can be treated appropriately. However, identifying the presence of calcium and/or potassium by EDAX can give an important indication of the underlying problem. For example, calcium, present as amorphous hydroxyl apatite, may indicate a malfunction of the dog's parathyroid gland. If a dog presents a bladder calculus, it may be of value to routinely check the urine for pH as well as for ammonia, which may indicate infection. An excessively high result for potassium may be an indicator for the underlying cause of calculus formation.

 

Conclusions

We offer the above experiences as a salutary example to other researchers who may be using the X-ray powder diffraction technique. Do not be misled (as we were initially) by 'missing lines' and 'wrong intensities'. These anomalies are not uncommon, and their identification may provide valuable hints of minor but important complexities associated with the condition that generated the stone. Should any readers wish to have urinary calculi analysed by XRD (canine, feline or human), we would be pleased to have them as contributors to this continuing research project.

We thank the University of KwaZulu-Natal for supporting this research project and providing access to the XRD and SEM EDAX equipment. We acknowledge the assistance of the late F. Graham, and D. Rabie (Umbilo Veterinary Clinic) as well as the many pet owners who have donated the calculi removed from their dogs.

 

1. Gray D., Laing M., Nel F. and Naude J.H. (1982). Composition of urinary calculi collected in the Durban area. S. Afr. Med. J. 61, 121–125.         [ Links ]

2. Laing M., Kerr A. and Nel F. (1993). Fifteen years and 5000 X-ray patterns later: renal stone analysis at the University of Natal, Durban. S. Afr. J. Sci. 89, 528–530.         [ Links ]

3. Laing M. and Dunlevey J. (2000). A novel urinary calculus from Felis catus. S. Afr. J. Sci. 96, 220–221.         [ Links ]

4. JCPDS (1997). Powder Diffraction File 15–762. ICDD, Walnut Street, Philadelphia, PA.         [ Links ]

5. Banks E., Chianelli R. and Korenstein R. (1975). Crystal chemistry of struvite analogs of the type MgMPO₄·6H₂O(M⁺ =K⁺,Rb⁺,Cs⁺,Tl⁺,NH₄⁺). Inorg. Chem. 14, 1634–1639.         [ Links ]

6. Mathew M. and Schroeder L.W. (1979). Crystal structure of a struvite analogue MgKPO₄·6H₂O. Acta Cryst. B35, 11–13.         [ Links ]

7. Whitaker A. and Jeffery J.W. (1970). The crystal structure of struvite MgNH₄PO₄·6H₂O. Acta Cryst. B26, 1429–1440.         [ Links ]

8. Huheey J.E., Keiter E.A. and Keiter R.L. (1993). Inorganic Chemistry, 4th edn. HarperCollins, New York.         [ Links ]

9. JCPDS (1997). Powder Diffraction File patterns 15–762 and 35–812. ICDD, Walnut Street, Philadelphia, PA.         [ Links ]

10. Abbona F., Calleri M. and Ivaldi G. (1984). Synthetic struvite MgNH₄PO₄·6H₂O correct polarity and surface features of some complementary forms. Acta Cryst. B40, 223–227.         [ Links ]

 

 

Received 6 June. Accepted 20 August 2008.

 

 

This article is accompanied by supplementary analytical and crystallographic results in tabular and graphical form online at www.sajs.co.za
* Author for correspondence. E-mail: dunleveyj@ukzn.ac.za

 

 

Supplementary material to:

Dunlevey J. and Laing M. (2008). Struvite infection calculi in dogs: Problems with urinary calculus identification, and the value of the results. S. Afr. J. Sci. 104, 471–472.