RESEARCH LETTERS

 

Fungus gnats and other Diptera in South African forestry nurseries and their possible association with the pitch canker fungus

 

 

Brett P. Hurley^{I, II}; Prem Govender^I; Teresa A. Coutinho^II; Brenda D. Wingfield^II; Michael J. Wingfield^II

^IDepartment of Zoology and Entomology, University of Pretoria, Pretoria 0002, South Africa
^IIForestry and Agricultural Biotechnology Institute, University of Pretoria

 

 

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ABSTRACT

Fusarium circinatum is the causal agent of a serious disease of seedlings in South African pine nurseries. Insects, especially fungus gnats (Diptera: Sciaridae, Mycetophlidae), are suspected of transmitting this fungus in nurseries. The aim of this study was to ascertain which species of gnats are present in South African pine nurseries, and to consider whether these and other Diptera carry F. circinatum. Dipteran fauna were surveyed in four major forestry nurseries between 2000 and 2001. Fungi were isolated from these flies and the resulting Fusarium species were identified. Bradysia difformis was the only fungus gnat species found and it occurred in all nurseries. Other Dipteran families collected included Agromyzidae, Cecidomyiidae, Chironomidae, Ephydridae, Muscidae, Simulidae and Tachinidae. This is the first report of B. difformis in South Africa. Fusarium circinatum was not isolated from any of the Diptera collected. Fusarium oxysporum and F. stil-boides were isolated from Chironomidae, but these fungi are not considered important pathogens in the nurseries surveyed.

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References

1. Binns E.S. (1981). Fungus gnats (Diptera: Mycetophilidae/Sciaridae) and the role of mycophagy in soil: a review. Rev. Ecol. Biol. Sol. 18, 77-90.         [ Links ]

2. Barraclough D.A. and Londt J.G.H. (1996). Diptera. In Insects of Southern Africa, eds C.H. Scholtz and E. Holm, pp. 283-321. University of Pretoria, Pretoria.         [ Links ]

3. Gillespie D.R. and Menzies J.G. (1993).Fungus gnats vector Fusarium oxysporum f.sp. radicis-lycopersici. Ann. Appl. Biol. 123, 539-544.         [ Links ]

4. Springer T.L. (1995). Fungus gnat (Diptera: Sciaridae) feeding damage to legume seedlings. J. Kansas Entomol. Soc. 68, 240-242.         [ Links ]

5. EickerA. and Ludick E. (1993). Methoprene for the control of Lycoriellaauripila sciarid infestation of the cultivated mushroom Agaricus bisporus. Mushroom Res. 2, 19-24.         [ Links ]

6. Gouge D.H. and Hague N.G.M. (1995). Glasshouse control of fungus gnats, Bradysia paupera on fuchsias by Steinernema feltiae. Fundam. Appl. Nematol. 18, 77-80.         [ Links ]

7. Jarvis W.R., Shipp J.L. and Gardner R.B. (1993). Transmission of Pythium aphanidermatum to greenhouse cucumber by the fungus gnat Bradysia impatiens (Diptera: Sciaridae). Ann. Appl. Biol. 122, 23-29.         [ Links ]

8. Leath K.T and Newton R.C. (1969). Interaction of a fungus gnat, Bradysia sp. (Sciaridae) with Fusarium spp. on alfalfa and red clover. Phytopathology 59, 257-258.         [ Links ]

9. James R.L., Dumroese R.K. and Wenny D.L. (1995). Botrytis cinerea carried by adults fungus gnats (Diptera: Sciaridae) in container nurseries. Tree Plant. Notes 46, 48-53.         [ Links ]

10. Landis T.D. (1996). Biocontrols for fungus gnats. For. Nurs. Notes 1, 18-19.         [ Links ]

11. Springer T.L. and Carlton C.E. (1993). Oviposition preference of darkwinged fungus gnats (Diptera: Sciaridae) among Trifolium species. J. Econ. Ent. 86, 1420-1423.         [ Links ]

12. Harris M.A. Oetting R.D. and Gardner W.A. (1995). Use of entomopathogenic nematodes and a new monitoring technique for control of fungus gnats, Bradysia coprophila (Diptera: Sciaridae), in floriculture. Biol. Control 5, 412-418.         [ Links ]

13. Anas O. and Reeleder R.D. (1988). Feeding habits of larvae of Bradysia coprophila on fungi and plant tissue. Phytoprotection 69, 73-78.         [ Links ]

14. Binns E.S. (1981). Sciarids as migrants. Mushroom J. 108, 415-423.         [ Links ]

15. Hungerford H.B. (1916). Sciara maggots injurious to potted plants. J. Econ. Ent. 9, 538-553.         [ Links ]

16. KennedyM.K. (1974). SurvivalanddevelopmentofBradysiaimpatiens(Diptera: Sciaridae) on fungal and non-fungal food sources. Ann. Ent. Soc. Am. 67, 745-749.         [ Links ]

17. Springer T.L. (1995). Vulnerability of pasture and range legumes to fungus gnats. Crop Sci. 35, 534-536.         [ Links ]

18. Gardner R.B., Jarvis W.R. and Shipp J.L. (1990). Ingestion of Pythium spp. by larvae of the fungus gnat Bradysia impatiens (Diptera: Sciaridae). Ann. Appl. Biol. 116, 205-212.         [ Links ]

19. Whipps J.M. and Budge S.P. (1993). Transmission of the mycoparasite Coniothyrium minitans by collembolan Folsomia candida (Collembola: Entomobryidae) and glasshouse sciarid Bradysia sp. (Diptera: Sciaridae). Ann. Appl. Biol. 124, 165-171.         [ Links ]

20. Kalb D.W. and Millar R.L. (1986). Dispersal of Verticillium albo-atrum by the fungus gnat (Bradysia impatiens). Plant Dis. 70, 752-753.         [ Links ]

21. Viljoen A., Wingfield M.J. and Marasas W.F.O. (1994). First report of Fusarium subglutinansf. sp. pini on pine seedlings in South Africa. Plant Dis. 78, 309-312.         [ Links ]

22. Wingfield M.J., Jacobs A., Coutinho T.A., Ahumda R. and Wingfield B.D. (2002). First report of the pitch canker fungus, Fusarium circinatum, on pines in Chile. Plant Pathol. 51, 397.         [ Links ]

23. Barnard E.L. and Blakeslee, G.M. (1980). Pitch canker of slash pine seedlings: a new disease in forest tree nurseries. Plant Dis. 64, 695-696.         [ Links ]

24. Rowan S.J. (1982). Tip dieback in southern pine nurseries. Plant Dis. 66, 258-259.         [ Links ]

25. Goldberg N.P. and Stanghellini M.E. (1990). Ingestion-egestion and aerial transmission of Pythium aphanidermatum by shore flies (Ephydrinae: Scatella stagnalis). Phytopathology 80, 1244-1246.         [ Links ]

26. Borror D.J. and White R.E. (1970). Insects. Houghton Mifflin, New York.         [ Links ]

27. Mcalpine J.F. (1981). Key to Families - Adults. In Manual of Nearctic Diptera, pp. 89-125. Biosystematics Research Institute, Ottawa.         [ Links ]

28. Barraclough D.A. (1995). An illustrated identification key to the acalyptrate fly families (Diptera: Schizophora) occurring in southern Africa. Ann. Natal Mus. 36, 97-133.         [ Links ]

29. Nash S.M. and Snyder W.C. (1962). Quantitative estimations by plate counts of propagules of the bean root rot Fusarium in field soils. Phytopathology 52, 567-572.         [ Links ]

30. Nirenberg H.I. and O'Donnell K.O. (1998).New Fusarium species and combinations within the Gibberela fujikuroi species complex. Mycology 90, 434-458.         [ Links ]

31. Nelson P.E., Toussoun T.A. and Marasas W.F.O. (1983). Fusarium Species: An Illustrated Manual for Identification. Pennsylvania University Press, Philadelphia.         [ Links ]

32. Rudzinski H.G. (1997). New sciarid flies from Africa (Diptera: Sciaridae). Entomol. Zeit. 107, 160-172.         [ Links ]

33. Rudzinski H.G. (1997). The Sciaridae (Diptera: Nematocera) of the Natal Museum, Pietermaritzburg (South Africa). Part 1. Entomol. Zeit. 107, 26-29.         [ Links ]

34. Rudzinski H.G. (1997). The Sciaridae (Diptera: Nematocera) of the Natal Museum, Pietermaritzburg (South Africa). Part 2. Entomol. Zeit. 107, 513-518.         [ Links ]

35. Menzel F., Smith J.E. and Colauto N.B. (2003). Bradysia difformis Frey and Bradysia ocellaris (Comstock): Two additional neotropical species of black fungus gnats (Diptera: Sciaridae) of economic importance: a redescription and review. Ann. Ent. Soc. Am. 96, 448-457.         [ Links ]

36. White P.F., Smith J.E. and Menzel F. (2000). Distribution of Sciaridae (Dipt.) species infesting commercial mushroom farms in Britain. Ent. Mon. Mag. 136, 207-209.         [ Links ]

37. Hellqvist S. (1994). Biology of Synacra sp. (Hym., Diapriidae), a parasitoid of Bradysia paupera (Dipt., Sciaridae) in Swedish greenhouses. J. Appl. Ent. 117, 491-497.         [ Links ]

38. Keates S.E., Sturrock R.N. and Sutherland J.R. (1989). Populations of adult fungus gnats and shore flies in British Columbia container nurseries as related to nursery environment, and incidence of fungi on the insects. New For. 3,1-9.         [ Links ]

39. Harris M.A., Gardner W.A. and Oetting R.D. (1996). A review of the scientific literature on fungus gnats (Diptera: Sciaridae) in the genus Bradysia. J. Entomol. Sci. 31, 252-276.         [ Links ]

40. Foote B.A. (1995). Biology of shore flies. Ann. Rev. Ent. 40, 417-442.         [ Links ]

41. Hesler L.S. (1995). Bibliography on Hydrellia griseola Fallen (Diptera: Ephydridae) and review of its biology and pest status. Insecta Mundi 9, 25-35.         [ Links ]

42. Corbaz R. and Fischer S. (1994). The shore fly Scatella stagnalis Fall. (Diptera, Ephydridae)responsiblefortransmittingFusariumoxysporumf. sp. lycopersiciin soiless tomatoe crops. Rev. suisse Vitic. Arboric. Hortic. 26, 383-385.         [ Links ]

43. Stanghellini M.E., Rasmussen S.L. and Kim D.H. (1999). Aerial transmission of Thielaviopsis basicola, a pathogen of corn-salad, by adult shore flies. Phytopathology 89, 476-479.         [ Links ]

44. Slaymaker A.K., Ferrington L.C.J. and Lichtwardt R.W. (1998). Chironomid-Trichomycete associations: a literature review. J. Kansas Entomol. Soc. 71, 490-500.         [ Links ]

45. Ocamb C.M. and Juzwik J. (1995). Fusarium species associated with rhizosphere soil and diseased roots of eastern white pine seedlings and associated nursery soil. Can. J. Plant Pathol. 17, 325-330.         [ Links ]

46. Bloomberg W.J. (1971). Diseases of Douglas-fir caused by Fusarium oxysporum. Phytopathology 61, 467-470.         [ Links ]

47. Bloomberg W.J. (1976). Distribution and pathogenicity of Fusarium oxysporum in a forest nursery soil. Phytopathology 66, 1090-1092.         [ Links ]

48. Phiri N.A., Hillcocks R.J. and Jeffries P. (2001). Incidence and severity of coffee diseases in smallholder plantations in northern Malawi. Crop Prot. 20,325-332.         [ Links ]

49. Ismail M.A. (2000). Pathogenicity ability and pectolytic enzymes of fungi most commonly associated with passion fruits with special reference to the effectiveness of sodium hypochlorite on the rot development. Afr. J. Mycol. Biotechnol. 8, 13-24.         [ Links ]

50. Zhang L. (2000). Recent situation and control of bamboo diseases in China. Ind. J. For. 23, 104-109.         [ Links ]

 

 

Received 16 November 2006.
Accepted 20 February 2007.

 

 

* Author for correspondence. E-mail: brett.hurley@fabi.up.ac.za