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Onderstepoort Journal of Veterinary Research

On-line version ISSN 2219-0635
Print version ISSN 0030-2465

Onderstepoort j. vet. res. vol.80 n.1 Pretoria Jan. 2013

 

RESEARCH COMMUNICATION

 

Biliary and plasma copper and zinc in pregnant Simmental and Angus cattle

 

 

S. Ravi GooneratneI, II; Bernard. LaarveldII; Kumar.K. PathiranaI; David.A. ChristensenII

IFaculty of Agriculture and Life Sciences, Lincoln University, New Zealand
IIDepartment of Animal and Poultry Science, University of Saskatchewan, Canada

Correspondence

 

 


ABSTRACT

Three each of 3-year-old Angus and Simmental heifers, surgically modified to collect bile, were used to measure the effects of pregnancy and breed on bile flow, biliary copper and zinc excretion and plasma copper and zinc concentrations. Bile copper excretion was significantly higher at 7-mo of pregnancy when samples from both breeds were pooled. From then onwards it declined to its lowest, one week post-partum. During pregnancy, plasma copper concentration increased slightly, reaching its highest level at 7-mo of pregnancy and then decreased slightly until full term. In pooled samples from both breeds, the correlation between increase in bile copper excretion and plasma copper concentration from 0 to 7-mo of pregnancy was high (r = 0.85) and significant (p < 0.05). Plasma zinc concentration decreased to the lowest level around 6-mo of pregnancy but increased thereafter until full term. In cows that were dried off one week after parturition, major shifts in bile and plasma copper and zinc parameters occurred at one week following and these coincided with a marked decline of bile flow and bile copper and zinc excretion. By 3-mo post-partum, biliary copper and zinc excretion and plasma copper and zinc concentrations had reached levels observed prior to pregnancy. When the data from all samples were pooled, the bile flow and bile copper excretion were significantly (p < 0.05) higher in Simmental, and plasma copper and zinc concentration higher in the Angus.


 

 

Introduction

Changes in copper (Cu) and zinc (Zn) metabolism occur during pregnancy to meet the demands of the foetus. Homeostasis of these two essential trace minerals in the pregnant animal depends on the relative absorption, excretion and their transfer to the foetus (Gooneratne, Buckley & Christensen 1989). High Cu content in most new-born animals suggests placental transfer and foetal liver storage before birth (Cunningham 1931; Gooneratne 1986; Gooneratne & Christensen 1989a, 1989b; Hefnawy et al. 2011; Pryor 1964) with foetal liver Cu concentration as high as 50% in the human foetus (Widdowson 1974). Early work indicated that bile is the major excretory route for many trace minerals (Evans 1973) and 94% of the excreted Cu is via faeces (Moss et al. 1974). According to the kinetic modelling of Buckley (1991), 61.4% of the total Cu excreted in faeces in non-pregnant cattle is from bile. Since foetal Cu and Zn are derived from the blood of the dam, maintaining the concentrations of these two essential trace elements in the maternal plasma during pregnancy, and if possible reducing their excretion, would be of critical importance. Requirements of Simmental are higher than Angus (Mullis, Spears & McGraw 2003), and Smart and Gudmundson (1980) suggested that Simmental cattle are more susceptible to Cu deficiency than other breeds. More recently, Hansen, Richter and Drewnoski (2010) reported that Angus and Simmental calves exhibit differential trace mineral metabolism, with Simmental showing lower plasma Cu concentration at 10 h post-injection of a mineral mix compared to Angus, probably because of increased Cu excretion via bile in this breed (Gooneratne et al. 1994). Zinc at high concentrations (Bremner, Young & Mills 1976) can affect Cu absorption and therefore Cu balance, especially when large doses of Zn salts are given as a treatment for facial eczema (Morris, Amyes & Hickey 2006). Zinc does not significantly influence the Cu-Mo-S interaction except at very high doses. In the present study (carried out at the Main Barn. Department of Animal and Poultry Science, University of Saskatchewan), the Cu and Zn excretion in bile and the plasma Cu, Zn concentrations of two breeds of cattle (Angus and Simmental) were investigated during different physiological stages: non-pregnant, pregnant and post-partum.

 

Materials and methods

Handling and care of cattle were in accordance with the principles and guidelines established by the Canadian Council on Animal Care (1984). Three each of 3-year-old Angus and Simmental heifers with average body weights of 409 kg and 431 kg were surgically modified for collection of bile as described by Symonds, Mather and Hall (1982) 1-mo prior to the experiment. Following surgery, all animals were kept in separate pens and fed Bromegrass hay (Bromus inermis Leyss.) (60%), barley (35%), canola meal (3%) based pelleted ration twice daily to meet National

Research Council (NRC) (1980) nutrient requirements. The diet contained 40 mg/kg, 57 mg/kg, 93 mg/kg, 42 mg/kg and 1 mg/kg of, Cu (added as copper oxide), Zn, iron (Fe), manganese (Mn) and molybdenum (Mo) respectively, and 0.2% sulphur (S). The animals were fed 7 kg of feed daily at the beginning of experiment, increasing weekly by 0.1 kg - 0.2 kg to allow for body weight gain, and had free access to drinking water from a municipal source. The higher Cu level of 40 mg/kg was recommended by Gooneratne (1986) for pregnant Simmental cattle in Western Canada, which is moderately higher than that recommended by Suttle (2010) for pregnant animals to allow the build-up of a large foetal reserve of Cu. The typical sulphate concentration in the municipal drinking water is 101 mg/L. All animals were synchronised for breeding with prostaglandin, dinoprost trimethamine ('Lutealyse', Upjohn Co.) and artificially inseminated with semen of the respective breed. At one week after parturition, the calves were separated and the cows dried off.

Bile flow, biliary Cu and Zn excretion, and plasma Cu and Zn concentrations were measured commencing 1 month before pregnancy, monthly during pregnancy, and at 0.25-mo (one week), one, two and three months following parturition. On sampling days, the rate of bile flow was measured by collecting the bile secreted during consecutive 30 min periods between 09:00 and 15:00 each day, into a bag attached to each animal's flank as described by Gooneratne et al. (1994). The volume was measured and 2 mL removed for analysis whilst returning the remainder into the duodenum through a re-entrant cannula. A blood sample was taken from each animal 30 min prior to each bile sampling session. Cu and Zn concentrations in bile and plasma (1:4 dilution) were measured directly by atomic absorption spectrophotometer (Perkin-Elmer, Model 5000, Perkin Elmer Corp., Norwalk, CT, USA) as described by Gooneratne et al. (1994). Overall, Cu homeostasis is dependent on Cu absorption, tissue distribution, accumulation in both dam and foetus, and Cu excretion. Since biliary Cu excretion is the major route of Cu excretion, only the Cu excretion rates (bile Cu concentration x bile volume) will be presented here.

Statistics

The data were analysed using the general linear models procedure (Ray 1982). The effect of time of sampling (stage of pregnancy), breed, and pooled samples on the bile Cu excretion, bile Zn excretion and plasma Cu and Zn concentrations were analysed as a one-way analysis of variance (ANOVA). Next, the effect of time of sampling and breed on the same parameters was analysed as a two-way ANOVA. Parameter means and also means of pooled samples were compared using Duncan's multiple range test (Snedecor & Cochran 1980). Results were significantly different at p < 0.05. Regression and correlation analysis between selected parameters were also performed.

 

Results

Bile flow

Overall in pooled data, pregnancy did not significantly affect (p > 0.05) the bile flow (Table 1). It declined slightly initially, remained within a narrow range from the second month of pregnancy to full term, but declined markedly at 0.25-mo (one week) following parturition, which was significantly lower (p < 0.05) than the flow prior to and at one month of pregnancy. It increased gradually thereafter to reach near initial non-pregnant and early gestation levels by 3-mo post-partum. Overall, mean bile flow in Simmental (2.60 L/6 h) tended to be greater than that in Angus (2.12 L/6 h) (Figure 1), and when samples were pooled by breed for the duration of the study, this difference was highly significant (p < 0.01). The month χ breed interaction was also significant (p < 0.05).

 

 

Biliary copper excretion

Overall, pregnancy did not affect biliary Cu excretion (p > 0.05) (Figure 1). However, there was a tendency in Simmental for the Cu excretion to gradually increase from 2-mo of pregnancy especially at the end of second trimester and in Angus from the beginning of third trimester. Following parturition, the biliary Cu excretion declined (p < 0.05) to the lowest recorded level at 0.25-mo at the time the calves were weaned. It then gradually recovered to reach levels similar to non-pregnant and pregnant levels from one to three months post-partum. Overall, mean biliary Cu excretion was significantly (p < 0.05) higher in Simmental (0.55 mg/6 h) than in Angus (0.34 mg/6 h). Biliary Cu excretion profiles in the two breeds were compiled for analysis (Figure 1). A comparison of bile Cu excretion in pooled Simmental and Angus samples taken at each month was significantly higher (p < 0.05) at the 7th month of pregnancy compared to the excretion one week after parturition.

Biliary zinc excretion

Month of sampling showed no effect on biliary Zn excretion (Figure 2). The biliary Zn excretion between the breeds was variable throughout pregnancy. In both breeds, it remained within a narrow range, declining slightly in the second trimester, which was most marked in Simmental, and declining again at 0.25-mo (one week) following parturition in both breeds at the time of weaning of calves, compared to the value at 9-mo of pregnancy and then continued to increase from then onwards, with the highest concentration recorded in Angus at 2-mo and in pooled samples at 3-mo after parturition. The month of sampling, breed and month χ breed interaction were not significant (p > 0.05).

 

 

Plasma copper concentration

The breed- and pooled-plasma Cu concentrations are shown in Figure 1 and Table 1 respectively. In both breeds, gestation or parturition had no significant effect on plasma Cu concentration, except for a high concentration at the beginning of the third trimester. When the samples from both breeds were pooled, the plasma concentration was significantly (p < 0.05) higher at 7-mo compared to several other time points. By 8-mo of pregnancy, the concentration declined to early pregnancy concentrations and remained so until parturition. At 0.25-mo (one week) following parturition, plasma Cu concentration increased compared to the concentration at full term. Then it gradually declined to reach a concentration similar to that prior to conception and early pregnancy. During the experiment, Angus tended to have a higher plasma Cu concentration compared to Simmental.

Plasma zinc concentration

The overall mean plasma Zn concentration between the breeds during the experiment was similar, with a mean of 0.94 mg/ L in Angus vs. 0.97 mg/L in Simmental. For both breeds, the plasma Zn concentration remained within narrow limits during early pregnancy, declined during second trimester, gradually increased through the third trimester to full-term to reach the highest overall concentration one week (0.25-mo) post-partum. The plasma Zn concentration then declined (p < 0.05) again from 1-mo post-partum onwards to reach values closer to the non-pregnant and early gestation concentrations. Overall, in pooled samples, plasma Cu and Zn concentrations (Table 1; Figure 1 and Figure 2) showed a negative correlation (r = -0.66) until third trimester and a relatively high positive correlation thereafter (r = 0.9).

 

Discussion

To our knowledge, this is the first report on biliary Cu and Zn excretion in cattle prior to, during, and after pregnancy, and it also compares differences in these parameters in two breeds of cattle, Simmental and Angus. The experimental model used in this study was technically challenging. Only a small number of animals could be used because of the care required to maintain bile cannulae and duodenal re-entrant cannulae clean and without blockages for over one year and especially in pregnant animals where displacement of organs can occur during pregnancy. Only the pooled data (Table 1) showed statistically significant differences during certain sampling times. Statistical differences between the two breeds were not discernible, except for the significantly higher (p < 0.05) biliary Cu excretion in pooled samples by Simmental than Angus, due to the small number of experimental animals used in the study and the large variation between animals. So, the discussion on effects due to breed differences will be limited to trends.

The plasma Cu concentration in cattle fed a balanced diet is around 0.9 mg/L, (Simpson, Mills & Mader 1981) although breed differences exist. The pooled plasma Cu concentration observed in this study prior to pregnancy and post-partum was mostly above 1 mg/L. However, unlike in other studies, the diet in this study contained 40 mg Cu/kg, which might seem excessive but is the amount required in cattle in Western Canada to supply the requirements of the foetus without an excessive drain on the dam liver Cu (Gooneratne, Laarveld & Christensen, unpublished observations). This is because of the high sulphate in deep well water in the province, although the sulphur concentration in this study was not high. However, in non-pregnant Simmental and Angus cattle on a similar diet as in this study, the plasma Cu concentration was even higher, > 1.5 mg/L in both Simmental and Angus (Gooneratne et al. 1994). Copper accretion in bovine foetus and non-foetal conceptus increases markedly and linearly during the third trimester (House & Bell 1993). Thus, a relatively lower plasma Cu concentration especially during the last 2-mo of pregnancy observed in this study seems to reflect the drain of Cu to meet the foetal demand.

Angus compared to Simmental had a slightly higher plasma Cu concentration, at least up to month seven of gestation and at 0.25-mo, 2-mo and 3-mo post-parturition in the present study. This is supported by our previous studies (Gooneratne et al. 1994), by Crow et al. (1980), and more recently by Hansen et al. (2010) who showed that Simmental calves exhibited significantly lower plasma Cu concentrations at 10 h post-injection of Multimin (a mineral mix of Cu, Zn, Mn and selenium) compared to Angus calves, probably due to a greater clearance via bile (Gooneratne et al. 1994) and urine (Gooneratne et al. 2011). The higher dietary Cu supplementation (40 mg/kg) in the present study may have narrowed the plasma Cu concentration gap between the two breeds since Cu unsupplemented Angus compared to Cu supplemented Angus heifers show much higher differential plasma Cu concentrations compared to Simmental heifers when both breeds received the same diet (Ward, Spears & Gengelbach 1995), probably because Simmental excrete more Cu in bile than Angus (Gooneratne et al. 1994). These observations provide further evidence to explain the prevalence of Cu deficiency in Simmental compared to other breeds as observed by Smart (1984). The effect of breed on liver and/or plasma Cu concentration has also been reported for sheep by Woolliams et al. (1982) and for other cattle breeds by Miranda et al. (2006). Evidence for genetic variation of Cu metabolism in Angus, Simmental, and Charolais (Ward et al. 1995), and Hereford-sired cows and Simmental-sired cows (Smart & Christensen 1985) have also been reported.

In the foetus, the liver is the main site for Cu storage in humans (Widdowson 1974). In ruminants, about 40%-70% of total body Cu is in the foetal liver, depending on the diet (Woolliams et al. 1983). Therefore the changes in maternal and foetal liver Cu concentrations during gestation would help to explain concomitant changes in plasma Cu, since liver and plasma Cu concentrations in pregnant and parturient cows are closely related (Smart et al. 1986). Whilst dam liver Cu concentration decreases significantly from trimester one through two to three, foetal liver Cu concentration increases significantly throughout gestation in cattle (Gooneratne & Christensen 1989a) and pigs (Richards 1999), but the rate of accumulation in the foetus and depletion in the dam depends on the availability of Cu from the diet (Gooneratne et al. 1986a). In addition, the weight of the foetal liver increases significantly from about 7-mo gestation, which in combination with the significant increase in liver Cu concentration at that time results in a substantial increase in the total Cu accretion in the foetal liver (Graham et al. 1994) at the expense of the maternal pool in cattle (Gooneratne, Laarveld & Christensen, unpublished observations) and sheep (Hefnawy et al. 2011). If only the liver Cu concentrations and its accretion at different stages of gestation are considered, quantitative accretion may fail to be detected, particularly at times of rapid foetus and foetal liver growth. Thus Abdelrahman and Kincaid (1993) observed the highest foetal liver Cu accretion during the third trimester, but the Cu concentration in the liver was not different from the two previous trimesters because of the rapid foetal liver growth. However, according to Graham et al. (1994), there is little variation in the dam liver Cu concentration with stage of pregnancy, but that depends to a large extent on the dietary Cu, Mo and S concentrations, with high dietary Mo and S concentrations markedly reducing both the dam and foetal liver Cu concentration (Gooneratne et al. 1986a).

The sudden surge in plasma Cu concentration at the beginning of the last trimester may have been triggered by a high foetal demand, since the demand for many of the essential trace elements also changes markedly during the terminal stages of foetal development (Mills & Davies 1979), when most of the foetal growth occurs. The exact time this occurs could not be determined because in this study the samplings were carried out at monthly intervals, although it appears to be around the end of the second trimester or the beginning of third trimester. From the findings of Gooneratne et al. (1986a, 1986b), it appears that Cu is actively transported across the placenta from the dam to the foetus and that the storage of Cu by the growing foetus proceeds even when the dam is fed a Cu deficient diet, at the expense of maternal Cu reserves. The gradual reduction in plasma Cu concentration from 7-mo of gestation period and an associated reduction in biliary Cu excretion may have been due to increased foetal accretion of Cu and the homeostatic mechanisms of the dam operating to maintain the dam plasma Cu concentration at a reasonable level and conserve much needed Cu by minimising losses via bile during this time. At one week following parturition, the increased plasma Cu concentration in the dam may have been due to the sudden absence of foetal demand and/or to support lactation demands. Thus variations in plasma Cu concentrations under the present experimental conditions are a reflection of the net result of foetal demand and/or lactation and a lack of it following parturition and drying off of the cows at one week post-partum. Thus, the plasma Cu concentrations in pregnant cattle observed in this study are in agreement with those reported by Smart et al. (1986) and increased plasma Cu concentration in dams after calving reported by Gonzalez et al. (1988). However, although the maternal homeostatic adaptations to mineral demands are apparent, a clear interpretation seems difficult without corresponding foetal data and limited data in the literature on mineral excretion in bile prior to, during, and after pregnancy.

The range in bile flow rates observed in this study is consistent with the cattle studies of Symonds, Mather and Hall (1982) and Gooneratne et al. (1994) for non-pregnant cattle. However, during pregnancy there were changes, with a decline or steady bile flow in Simmental and Angus respectively during the 1st trimester, a relatively steady flow in both breeds during the second trimester, an increase during the third trimester, and a significant (p < 0.05) decline in pooled samples at one week after parturition compared to the last month of pregnancy, non-pregnant and mo-1 of pregnancy. In non-pregnant cattle, different combinations of dietary Cu, S and Mo can create significant effects on biliary flow (Gooneratne et al. 1994) whilst short-term intravenous infusions of Cu have minimal effect (Charmley & Symonds 1985). In contrast, the changes in bile flow observed in this study with pregnant cattle were probably a reflection of the demands at different physiological stages - non-pregnant, pregnant, and post partum.

In general, bile flow and biliary excretion of Cu tended to be higher in Simmental throughout the experimental period with few exceptions, with overall averages of 2.60 L/6 h and 0.55 mg/6 h for Simmental compared to 2.12 L/6 h and 0.34 mg/6 h for Angus. Gooneratne et al. (1994) found no significant difference between Simmental and Angus in the flow of bile of non-pregnant heifers, although it was relatively greater in Simmental. According to Mills and Davies (1979), biliary secretions in the dam during late foetal development are rich in Cu, and in the current study it peaked at 6-mo of pregnancy in Simmental and 7-mo in Angus (Figure 1). Plasma Cu concentration also followed a similar pattern, with significantly higher concentrations at the commencement of 6-mo and 7-mo of pregnancy. Thus, in most instances, both biliary Cu excretion and plasma Cu concentration increased with advancing gestation up to 6-mo or 7-mo of pregnancy (r = 0.85), but declined thereafter up to full-term and this was most marked in Simmental. At one week following parturition, biliary Cu excretion declined to a value significantly lower than the highest level observed at 7-mo, whereas plasma Cu concentration increased. Therefore, following parturition and up to 3-mo post-partum, Cu excretion in bile and plasma Cu concentration were again closely but inversely related (r = -0.9). Such correlations emphasise again that, in cattle, bile Cu excretion and plasma Cu concentration in the cows whilst closely related to each other, are strongly affected by the physiological stage, foetal demands and a shift in Cu-homeostasis mechanisms to maintain the demands of the foetus whilst attempting to maintain maternal Cu levels within desirable limits (Gooneratne & Christensen 1989a; Smart, Christensen & Shargool 1983). This interaction is even more complex in humans (Gambling, Anderson & McArdle 2008) because of the high prevalence of Fe deficiency in women during pregnancy. The decline in biliary Cu excretion post-partum could also be interpreted as a consequence of the drain of Cu into milk during lactation and the elevation of plasma Cu as a consequence of mobilisation of Cu reserves to achieve this and the absence of foetal demand. However, lactation in cows does not raise requirements significantly because of the low Cu content of milk (approximately 2 mg/L; Suttle 2010), although the demand from the dam increases, especially in high producing cows.

Copper and Zn interactions are well known (Bremner et al. 1976; Grace & Gooden 1980; Saylor, Morrow & Leach 1980; Tanabe 1980). However, the present experiment is the first investigation into the relationship of these two minerals in plasma and bile as affected by pregnancy rather than as a function of dietary intake. In pooled samples, plasma Zn was inversely correlated with bile Cu (r = -0.77) and plasma Cu was also negatively, but less closely related to bile Zn (r = -0.41). Liver Cu concentrations decrease following Zn injection in goat kids (Tanabe 1980) or feeding supplementary Zn to sheep (Saylor et al. 1980).

It appears that biliary Cu and Zn, and plasma Cu and Zn concentrations behave in a similar manner in both Simmental and Angus, albeit with some differences. In humans, the serum Cu concentration increases whilst serum Zn declines in the mother during pregnancy (Widdowson 1974) and plasma Cu and Zn concentrations were similar in this study also until the beginning of the third trimester. Thereafter until parturition, plasma Cu concentration decreased whilst plasma Zn concentration increased. Dufty, Bingley and Cove (1977), Smart et al. (1986) and Gonzalez et al. (1988) have reported similar decreases in plasma Zn concentration in cows as pregnancy advanced, but only Gonzalez et al. (1988) were able to show an increase in plasma Zn concentration soon after calving, as observed in this study. However, according to Hansard, Mohammed and Turner (1968), plasma Zn concentration in pregnant cross-bred heifers remains the same throughout pregnancy. It is possible that the higher Cu supplementation (40 mg/kg) used in the current study may have altered the Zn balance. The plasma Zn concentration during different stages of pregnancy depends on the species, breed or genetic constitution of the cattle, but more importantly on the overall mineral nutrition balance, including Cu and Zn supplementation during the gestation period. Highest plasma Zn concentration recorded one week after calving was associated with a low biliary excretion of Zn.

Trends which could not be fully explained in this study may be due to one or a combination of the following: (1) small sample size with only three cattle per breed, (2) lack of data on trace element concentrations, including Cu and Zn in pancreatic secretions (Grace & Gooden 1980), saliva (Stevenson & Unsworth 1978) and urine, all of which contribute to the overall Cu and Zn excretion, (3) lack of data in the literature on absorption and metabolism, excretion of Cu and Zn during pregnancy, and (4) a dearth of information on the other minerals that interact with Cu and Zn metabolism in pregnant cattle. Nevertheless, the evidence presented here points to partitioning of Cu due to an increased foetal demand, especially at the end of the second trimester and during the third trimester, and a marked decrease in both biliary Cu and Zn excretion one week postpartum, probably to make available more Cu and Zn to increase maternal plasma Cu and Zn concentration to support early lactation demand. These findings thus provide further support for higher mineral supplementation during pregnancy and especially from mid-gestation onwards as recommended for sheep, cattle, and deer by Grace and Knowles (2010), and especially during the last trimester of pregnancy although this has led to high liver Cu concentration in some dairy herds in New Zealand (Grace, Knowles & Hittman 2010).

 

Conclusion

In conclusion, the bile Cu excretion was significantly (p < 0.05) higher at 7-mo pregnancy and lowest one week post- partum in all cattle. In pooled samples from both breeds, the correlation between increase in bile Cu excretion and plasma Cu concentration from 0 to 7-mo of pregnancy was high and significant. Major shifts in bile and plasma Cu and Zn parameters occurred at one week following parturition. By 3-mo post-partum, biliary Cu and Zn excretion and plasma Cu and Zn concentrations had reached levels observed prior to pregnancy. Bile flow and bile Cu excretion were significantly (p < 0.05) higher in Simmental, and plasma Cu and Zn concentration higher in the Angus.

 

Acknowledgements

The authors thank T. Berryere for care of animals and technical assistance. This work was supported by the Saskatchewan Agriculture Development Fund and Natural Sciences and Engineering Research Council of Canada.

Competing interests

The authors declare that they have no financial or personal relationship(s) which may have inappropriately influenced them in writing this article.

Authors' contributions

S.R.G. (Lincoln University) was the project leader, performed most of the experiments, and wrote most of the manuscript. B.L. and D.A.C. (University of Saskatchewan) were responsible for experimental and project design. K.K.P. (Lincoln University) was responsible for data analysis and writing some parts of the manuscript.

 

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 Correspondence:
Ravi Gooneratne
Email: ravi.gooneratne@lincoln.ac.nz
Postal address: PO Box 84, Lincoln University, Lincoln 7647, New Zealand

Received: 15 Feb. 2013
Accepted: 25 June 2013
Published: 26 Aug. 2013

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