SciELO - Scientific Electronic Library Online

 
vol.79 número2Tuberculosis cross-species transmission in Tanzania: Towards a One-Health conceptFiloviral haemorrhagic fevers: A threat to Zambia? índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Articulo

Indicadores

Links relacionados

  • En proceso de indezaciónCitado por Google
  • En proceso de indezaciónSimilares en Google

Compartir


Onderstepoort Journal of Veterinary Research

versión On-line ISSN 2219-0635
versión impresa ISSN 0030-2465

Onderstepoort j. vet. res. vol.79 no.2 Cape Town ene. 2012

 

ABSTRACT

 

Identification of the plague reservoir in an endemic area of Zambia

 

 

Bernard M. Hang'ombeI; I. NakamuraII; D. KaileIII; A.S. MweeneI; K.L. SamuiI; B.S. KilonzoIV; H. SawaII; C. SugimotoII; B. WrenV

ISchool of Veterinary Medicine, University of Zambia, Zambia
IIResearch Center for Zoonosis Control, Hokkaido University, Japan
IIINamwala District Medical Officer, Namwala District, Zambia
IVSokoine University of Agriculture, United Republic of Tanzania
VSchool of Hygiene and Tropical Medicine, London, United Kingdom

Correspondence to

 

 

Yersinia pestis, the bacterial agent of plague, is primarily a parasite of wild rodents that persists in permanent, discrete enzootic foci throughout the world. The disease is transmitted in humans by bites from fleas of wildlife rodent species. Therefore surveillance is the ultimate public health solution through plague detection in domestic dogs, other carnivores and wild rodents. The investigations of die-offs amongst plague-susceptible colonial rodents are also significant to determine the presence of Y. pestis in a susceptible population.

This study details the identification of the plague reservoir in a suspected endemic area of Zambia. The study was undertaken through rodent investigation for the presence of Y. pestis. A total of 105 rodents were sampled routinely and during a suspected plague period. On dissection 4 (3.81%, 95% CI: 1.23-10.0) rodents sampled during an outbreak showed signs of spleen enlargement. The blood, liver, lymph nodes and spleen of each rodent were subjected to culture on 6% sheep blood agar and MaCconkey agar. Colonies obtained were identified as Y. pestis by colony morphologic features, biochemical profiles, mouse inoculation assay and polymerase chain reaction (PCR). The PCR primers used targeted the Y. pestis plasminogen activator gene, chromosomal ferric iron uptake regulation gene and the outer membrane protein B gene.

The isolates were also subjected to antibiotic sensitivity tests using the disk diffusion method on Mueller-Hinton agar with sensitivity being observed with ampicillin, amoxicillin, chloramphenicol, gentamycin, streptomycin, tetracycline and trimethoprim-sulfamethoxazole. The findings, identifies a natural reservoir of Y. pestis in Zambia providing the public health officials with a definite host for the control strategy.

 

 

Correspondence to:
Bernard Hang'ombe
PO Box 32379, Lusaka, Zambia
Email: bernard.hang'ombe@sacids.org

 

 

Note: Proceedings of the Conference of the Southern African Centre for Infectious Disease Surveillance 'One Health' held at the National Institute for Communicable Diseases, Johannesburg, July 2011.

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons