SciELO - Scientific Electronic Library Online

 
vol.76 número3 índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Artigo

Indicadores

Links relacionados

  • Em processo de indexaçãoCitado por Google
  • Em processo de indexaçãoSimilares em Google

Compartilhar


Onderstepoort Journal of Veterinary Research

versão On-line ISSN 2219-0635
versão impressa ISSN 0030-2465

Onderstepoort j. vet. res. vol.76 no.3 Pretoria  2009

 

ORIGINAL RESEARCH

 

The effect of pyridoxal-5-phosphate on serum alanine aminotransferase activity in dogs suffering from canine babesiosis

 

 

E.C. Myburgh; A. Goddard

Department of Companion Animal Clinical Studies, Section of Clinical Pathology, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110 South Africa

 

 


ABSTRACT

Accurate measurements of serum aminotransferase (ALT) activity in dogs relies on the endogenous pro-enzyme pyridoxal 5-phosphate (P5P). The purpose of this study was to determine whether the exclusion of P5P from the analytical method causes an underestimation of serum ALT activity in dogs suffering from babesiosis and in those manifesting evidence of hepatocellular damage, and to determine if anorexia causes sufficient P5P depletion to affect in vitro serum ALT activity. One-hundred-and-twenty healthy control dogs and 105 Babesia-infected dogs were included in the study. Two methods for ALT measurement were used: Method 1 included P5P, and Method 2 excluded P5P from the reaction mixture. Higher serum ALT activity was measured with Method 1 in the Babesia-infected dogs (P < 0.001), as well as in 14 dogs with suspected hepatocellular damage (P = 0.03). Duration of anorexia had no effect, irrespective of the method used. Although inclusion of P5P to the reaction mixture consistently resulted in higher measured serum ALT activity, the differences were too small to have led to incorrect diagnoses in the Babesia-infected dogs suspected of liver disease.

Keywords: Alanine aminotransferase, anorexia, babesiosis, liver disease, pyridoxal 5'-phosphate


 

 

“Full text available only in PDF format”

 

 

REFERENCES

ALTMAN, D.G. & BLAND, J.M. 1983. Measurement in medicine: the analysis of method comparison studies. The Statistician, 32:307-317.         [ Links ]

BERGMEYER, H.U., SCHEIBE, P. & WAHLEFELD, A.W. 1978. Optimization of methods for aspartate aminotransferase and alanine aminotransferase. Clinical Chemistry, 24:58-73.         [ Links ]

CONSOLAZIO, C.F., JOHNSON, H.L., KRZYWICKI, H.J., DAWS, T.A. & BARNHART, R.A. 1971. Thiamin, riboflavin, and pyridoxine excretion during acute starvation and calorie restriction. American Journal of Clinical Nutrition, 24:1060-1067.         [ Links ]

HOPKINS, W.G. 2004. Bias in Bland-Altman but not regression validity analyses. Sportscience, 8:42-46.         [ Links ]

HORDER, M. & BOWERS, G.N. 1977. Biological variability in aspartate aminotransferase activity in serum of healthy persons, and effect of in vitro supplementation with pyridoxal 5-phosphate. Clinical Chemistry, 23:551-554.         [ Links ]

JACOBSON, L.S. & CLARK, I.A. 1994. The pathophysiology of canine babesiosis: New approaches to an old puzzle. Journal of the South African Veterinary Association, 65:134-145.         [ Links ]

JACOBSON, L.S. 2006. The South African form of severe and complicated canine babesiosis: Clinical advances 1994-2004. Veterinary Parasitology, 138:126-139.         [ Links ]

LOBETTI, R.G. 1998. Canine babesiosis. Compendium of Continuing Education, 20:418-430.         [ Links ]

MARSH, M.E., GREENBERG, C.D. & RINEHART, J.F. 1955. The relationship between pyridoxine ingestion and transaminase activity. Journal of Nutrition, 56:115.         [ Links ]

MATJILA, P.T., LEISEWITZ, A.L., JONGEJAN, F. & PENZHORN, B.L. 2008. Molecular detection of tick-borne protozoal and ehrlichial infections in domestic dogs in South Africa. Veterinary Parasitology, 155:152-157.         [ Links ]

MESHER, C.I., REJ, R. & STOKOL, T. 1998. Alanine amino-transferase apoenzyme in dogs. Veterinary Clinical Pathology, 27:26-30.         [ Links ]

NANJI, A.A. 1983. Decreased activity of commonly measured serum enzymes: Causes and clinical significance. American Journal of Medical Technology, 49:241-245.         [ Links ]

REJ, R., RUDOFSKY, U., MAGRO, A. & PRENDERGAST, J. 1990. Effects of exercise on serum amino-transferase activity and pyridoxal phosphate saturation in Thoroughbred racehorses. Equine Veterinary Journal, 22:205-208.         [ Links ]

REYERS, F., LEISEWITZ, A.L., LOBETTI, R.G., MILNER, R.J. & JACOBSON, L.S. 1998. Canine babesiosis in South Africa: more than one disease. Does this serve as a model for falciparum malaria? Annals of Tropical Medicine & Parasitology, 92:503-511.         [ Links ]

STOKOL, T. & ERB, H. 1998. The apo-enzyme content of aminotransferases in healthy and diseased domestic animals. Veterinary Clinical Pathology, 27:71-78.         [ Links ]

SCHMID, M. & VON FORSTNER, L. 1986. Laboratory testing in veterinary medicine diagnosis and clinical monitoring, 3rd ed. Mannheim: Boehringer Mannheim GmbH.         [ Links ]

VANDERLINDE, R.E. 1986. Review of pyridoxal phosphate and the transaminases in liver disease. Annals of Clinical and Laboratory Science, 16:79-93.         [ Links ]

VINYARD, E., JOVEN, C.B., SWENDSEID, M.E. & DRENICK, E.J. 1967. Vitamin B6 nutriture studied in obese subjects during 8 weeks of starvation. The American Journal of Clinical Nutrition, 20:317-323.         [ Links ]

WESTERHUIS, L.W.J.J.M. & HAFKENSCHEID, J.C.M. 1983. Apoenzyme content of serum aminotransferases in relation to plasma pyridoxal-5-phosphate concentration. Clinical Chemistry, 29:789-792.         [ Links ]

 

 

Accepted for publication 16 March 2009-Editor

 

 

* Author to whom correspondence is to be directed. E-mail: amelia.goddard@up.ac.za

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons