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Onderstepoort Journal of Veterinary Research

On-line version ISSN 2219-0635

Onderstepoort j. vet. res. vol.76 n.2 Cape Town  2009

 

ORIGINAL RESEARCH

 

Seroprevalence of Cryptosporidium parvum infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23

 

 

T. InpankaewI; S. JittapalapongI; J. PhasukI; N. PinyopanuwutI; W. ChimnoII; C. KengradomkitI; C. SunantaII; G. ZhangIII; G.O. AbogeIII; Y. NishikawaIII; I. IgarashiIII; X. XuanIII

IDepartment of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand
IIChiang Rai Provincial Office, Department of Livestock Development, Chiang Rai, Thailand
IIINational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan

 

 


ABSTRACT

Cryptosporidium parvum is the most frequent parasitic agent that causes diarrhoea in AIDS patients in Thailand. Cryptosporidiosis outbreaks in humans may be attributed to contamination of their drinking water from infected dairy pastures. A 23-kDa glycoprotein of C. parvum (CpP23) is a sporozoite surface protein that is geographically conserved among C. parvum isolates. This glycoprotein is a potentially useful candidate antigen for the diagnosis of cryptosporidiosis by enzyme-linked immunosorbent assay. Therefore, we investigated the seroprevalence of C. parvum infection in dairy cows in northern Thailand using an ELISA based on recombinant CpP23 antigen. Sera were randomly collected from 642 dairy cows of 42 small-holder farmers, which had the top three highest number of the dairy cows' population in Northern Thailand, that included Chiang Mai, Chiang Rai and Lumpang provinces. The overall seroprevalence of the infection was 4.4 %, and the seropositive rates for the three provinces were 3.3 % in Chiang Mai, 5.1 % in Chiang Rai and 3 % in Lumpang. These results suggest that cattle could play a role in zoonotic cryptosporidiosis in Thailand.

Keywords: Cryptosporidium parvum, CpP23, dairy cow, ELISA, Thailand


 

 

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REFERENCES

ABE, N., KIMATA, I. & ISEKI, M. 2002. Identification of genotypes of Cryptosporidium parvum isolated from a patient and dog in Japan. Journal of Veterinary Medical Science, 64:65-168.         [ Links ]

BANNAI, H., NISHIGAWA, Y., SEO, J., NAKAMURA, C., ZHANG, S., KIMATA, I., TAKASHIMA, Y., LI, J., IGARASHI, I. & XUAN, X. 2006. Development and evaluation of an enzyme-linked immunosorbent assay using recombinant p23 for the detection of antibodies to Cryptosporidium parvum in cattle. Journal of Protozoology Research, 16:9-15.         [ Links ]

BECHER, K.A., ROBERTSON, I.D., FRASER, D.M., PALMER, D.G. & THOMPSON, R.C. 2004. Molecular epidemiology of Giardia and Cryptosporidium infections in dairy calves originating from three sources in Western Australia. Veterinary Parasitology, 123:1-9.         [ Links ]

BOMFIM, T.C.B., HUBER, F., GOMES, R.S. & ALVES, L.L. 2005. Natural infection by Giardia spp. and Cryptosporidium spp. in dairy goats, associated with possible risk factors of the studied properties. Veterinary Parasitology, 134:9-13.         [ Links ]

CASTRO-HERMIDA, J.A., ALMEIDA, A., GONZÁLEZ-WAR-LETA, M., CORREIA DA COSTA, J.M., RUMBO-LORENZO, C. & MEZO, M. 2007. Occurrence of Cryptosporidium parvum and Giardia duodenalis in healthy adult domestic ruminants. Parasitology Research, 101:1443-1448.         [ Links ]

CURRENT, W.L. & GARCIA, L.S. 1991. Cryptosporidiosis. Clinical Microbiology Reviews, 4:325-358.         [ Links ]

FAYER, R., TROUT, J.M. & GRACZYK, T.D. 2000. Prevalence of Cryptosporidium, Giardia, Eimeria infections in post-weaned and adult cattle on three Maryland farms. Veterinary Parasitology, 93:103-112.         [ Links ]

GUERRANT, R.L. 1997. Cryptosporidiosis: an emerging, highly infectious threat. Emerging Infectious Diseases, 169:696-699.         [ Links ]

JITTAPALAPONG, S., PINYOPANUWAT, W., CHIMNOI, W., SIRIPANTH, C. & STICH, R.W. 2006. Prevalence of Cryptosporidium among dairy cows in Thailand. Annals of the New York Academy of Sciences, 1081:328-335.         [ Links ]

MANATSATHIT, S., TANSUPASAWASDIKUL, S., WANACHI-WANAWIN, D., SETAWARIN, S., SUWANAGOOL, P., PRAKASVEJAKIT, S., LEELAKUSOLWONG, S., EAMPOKALAP, B. & KACHINTORN, U. 1996. Causes of chronic diarrhea in patients with AIDS in Thailand: a prospective clinical and microbiological study. Journal of Gastroenterology, 31:533-537.         [ Links ]

NGUYEN, S.T., NGUYEN, D.T., LE, D.Q., LE HUA, L.N., VAN NGUYEN, T., HONMA, H. & NAKAI, Y. 2007. Prevalence and first genetic identification of Cryptosporidium spp. in cattle in central Viet Nam. Veterinary Parasitology, 150:357-361.         [ Links ]

PERRYMAN, L.E., KAPIL, S.J., JONES, M.L. & HUNT, E.L. 1999. Protection of calves against cryptosporidiosis with immune bovine colostrum induced by a Cryptosporidium parvum recombinant protein. Vaccine, 17:2142-2149.         [ Links ]

SAKAI, H., TSUSHIMA, Y., NAGASAWA, H., DUCUSIN, R.J., TANABE, S., UZUKA, Y. & SARASHINA, T. 2003. Cryptosporidium infection of cattle in the Tokachi district, Hokkaido. Journal of Veterinary Medical Science, 65:125-127.         [ Links ]

SAVIOLI, L., SMITH, H. & THOMPSON, A. 2006. Giardia and Cryptosporidium join the 'Neglected Disease Initiative'. Trends in Parasitology, 22:203-208.         [ Links ]

TAKASHIMA, Y., XUAN, X., KIMATA, I., ISEKI, M., KODAMA, Y., NAGANE, N., NAGASAWA, H., MATSUMOTO, Y., MIKAMI, T. & OTSUKA, H. 2003. Recombinant bovine herpes-virus-1 expressing p23 protein of Cryptosporidium parvum induces neutralizing antibodies in rabbits. Journal of Parasitology, 89:276-282.         [ Links ]

TROTZ WILLIAMS, L.A., JARVIE, B.D., MARTIN, S.W., LESLIE, K.E. & PEREGRINE, A.S. 2005. Prevalence of Cryptosporidium infection in Southwestern Ontario and its association with diarrhea in neonatal diary calves. Canadian Veterinary Journal, 46:349-351.         [ Links ]

 

 

Accepted for publication 14 August 2008-Editor

 

 

* Author to whom correspondence is to be directed. E-mail: gen@obihiro.ac.jp

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