RESEARCH COMMUNICATION

 

Sequence and phylogenetic analysis of chicken anaemia virus obtained from backyard and commercial chickens in Nigeria

 

 

D.O. Oluwayelu^I; D. Todd^II; O.D. Olaleye^III

^IDepartment of Veterinary Microbiology and Parasitology, University of Ibadan, Ibadan, Nigeria
^IIVeterinary Sciences Division, Department of Agriculture and Rural Development for Northern Ireland, Stormont, Belfast BT4 3SD, United Kingdom
^IIIDepartment of Virology, College of Medicine, University of Ibadan, Ibadan, Nigeria

 

 

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ABSTRACT

This work reports the first molecular analysis study of chicken anaemia virus (CAV) in backyard chickens in Africa using molecular cloning and sequence analysis to characterize CAV strains obtained from commercial chickens and Nigerian backyard chickens. Partial VP1 gene sequences were determined for three CAVs from commercial chickens and for six CAV variants present in samples from a backyard chicken. Multiple alignment analysis revealed that the 6 % and 4 % nucleotide diversity obtained respectively for the commercial and backyard chicken strains translated to only 2 % amino acid diversity for each breed. Overall, the amino acid composition of Nigerian CAVs was found to be highly conserved. Since the partial VP1 gene sequence of two backyard chicken cloned CAV strains (NGR/Cl-8 and NGR/Cl-9) were almost identical and evolutionarily closely related to the commercial chicken strains NGR-1, and NGR-4 and NGR-5, respectively, we concluded that CAV infections had crossed the farm boundary.

Keywords: Chicken anaemia virus, cloning, sequence analysis

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Accepted for publication 14 August 2008-Editor