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Onderstepoort Journal of Veterinary Research

On-line version ISSN 2219-0635
Print version ISSN 0030-2465

Onderstepoort j. vet. res. vol.75 n.3 Cape Town  2008

 

ORIGINAL RESEARCH

 

Detection of Anaplasma antibodies in wildlife and domestic species in wildlife-livestock interface areas of Kenya by major surface protein 5 competitive inhibition enzyme-linked immunosorbent assay

 

 

J.J.N. NgeranwaI, II; S.P. ShompoleI; E.H. VenterII; A. WambuguI; J.E. CraffordII; B.L. PenzhornII

IKenya Agricultural Research Institute, P.O. Box 29231, Nairobi, Kenya
IIDepartment of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onder-stepoort, 0110 South Africa

Correspondence

 

 


ABSTRACT

The seroprevalence of Anaplasma antibodies in wildlife (eland, blue wildebeest, kongoni, impala, Thomson's gazelle, Grant's gazelle, giraffe and plains zebra) and domestic animal (cattle, sheep and goat) populations was studied in wildlife/livestock interface areas of Kenya. Serum samples were analyzed by competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA), using a recombinant antigen (MSP-5) from Anaplasma marginale surface membrane. A monoclonal antibody, FC-16, was used as the primary antibody, while anti-mouse conjugated to horseradish peroxidase was used as the secondary antibody. The results indicate a high seroprevalence in both wildlife and livestock populations, in contrast to earlier reports from Kenya, which indicated a low seroprevalence. The differences are attributed to the accurate analytical method used (CI-ELISA), as compared with agglutination techniques, clinical signs and microscopy employed by the earlier workers.

Keywords: Anaplasma, CI-ELISA, Kenya, seroprevalence, wildlife-livestock interface


 

 

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Correspondence:
Department of Biochemistry & Biotechnology
Kenyatta University
P.O. Box 43844
Nairobi, Kenya

Accepted for publication 24 April 2008-Editor

 

 

* Author to whom correspondence is to be directed. E-mail: banie.penzhorn@up.ac.za

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