ORIGINAL RESEARCH

 

An immunohistochemical study of ovarian innervation in the emu (Dromaius novaehollandiae)

 

 

M-C. Madekurozwa

Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110 South Africa

 

 

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ABSTRACT

The present study investigated the distribution of nerves in the ovary of the emu. The neuronal markers, protein gene product 9.5, neurofilament protein and neuron specific enolase demonstrated the constituents of the extrinsic and intrinsic ovarian neural systems. The extrinsic neural system was composed of ganglia in the ovarian stalk, as well as nerve bundles, which were distributed throughout the ovary. Isolated neuronal cell bodies, in the medulla and cortex, formed the intrinsic neural system. An interesting finding of the study was the presence of nerve bundles, circumscribed by lymphocytes, in the ovarian stalk. The findings of the study indicate that the distribution of nerve fibres and neuronal cell bodies in the emu ovary is similar, but not identical to that of the domestic fowl and ostrich.

Keywords: Emu, immunohistochemistry, innervation, ovary

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REFERENCES

ANESETTI, G., LOMBIDE, P., D'ALBORA, H. & OJEDA, S.R. 2001. Intrinsic neurons in the human ovary. Cell and Tissue Research, 306:231-237.         [ Links ]

AGUADO, L.I. 2002. Role of the central and peripheral nervous system in the ovarian function. Microscopy Research and Technique, 59:462-473.         [ Links ]

BAHR, J., KAO, L. & NALBANDOV, A.V. 1974. The role of catecholamines and nerves in ovulation. Biology of Reproduction, 10:273-290.         [ Links ]

CASAIS, M., DELGADO, S.M., SOSA, Z. & RASTRILLA, A.M. 2006. Involvement of the coeliac ganglion in the luteotrophic effect of androstenedione in late pregnant rats. Reproduction, 131:361-368.         [ Links ]

CURRY, T.E., LAWRENCE, I.E. & BURDEN, H.W. 1984. Ovarian sympathectomy in the guinea pig. I. Effects on follicular development during the estrous cycle. Cell and Tissue Research, 236:257-263.         [ Links ]

D'ALBORA, H. & BARCIA, J.J. 1996. Intrinsic neuronal cell bodies in the rat ovary. Neuroscience Letters, 205:65-67.         [ Links ]

D'ALBORA, H., LOMBIDE, P. & OJEDA, S.R. 2000. Intrinsic neurons in the rat ovary: an immunohistochemical study. Cell and Tissue Research, 300:47-56.         [ Links ]

D'ALBORA, H., ANESETTI, G., LOMBIDE, P., DEES, W.L. & OJEDA, S.R. 2002. Intrinsic neurons in the mammalian ovary. Microscopy Research and Technique, 59:484-489.         [ Links ]

DEES, W.L., HINEY, J.K., SCHULTEA, T.D., MAYERHOFER, A., DANILCHIK, M., DISSEN, G.A. & OJEDA, S.R. 1995. The primate ovary contains a population of catecholaminergic neuron-like cells expressing nerve growth receptors. Endocrinology, 136:5760-5768.         [ Links ]

DEES, W.L., HINEY, J.K., McARTHUR, N.H., JOHNSON, G.A., DISSEN, G.A. & OJEDA, S.R. 2006. Origin and ontogeny of mammalian ovarian nerves. Endocrinology, 147:3789-3796.         [ Links ]

DELGADO, S.M., SOSA, Z., DOMINGUEZ, N.S., CASAIS, M., AGUADO, L. & RASTRILLA, A.M. 2004. Effect of relation between neural cholinergic action and nitric oxide on ovarian steroidogenesis in prepubertal rats. Journal of Steroid Biochemistry and Molecular Biology, 91:139-145.         [ Links ]

FELTEN, D.L., ACKERMAN, K.D., WIEGAND, S.J. & FELTEN, S.Y. 1987. Noradrenergic sympathetic innervation of the spleen: I. Nerve fibers associate with lymphocytes and macrophages in specific compartments of the splenic white pulp. Journal of Neuroscience Research, 18:28-36.         [ Links ]

GERENDAI, I., KOCSIS, K. & HALASZ, B. 2002. Supraspinal connections of the ovary: structural and functional aspects. Microscopy Research and Technique, 59:474-483.         [ Links ]

GILBERT, A.B. 1965. Innervation of the ovarian follicle of the domestic hen. Quarterly Journal of Experimental Physiology, 50:437-445.         [ Links ]

GILBERT, A.B. 1968. Innervation of the avian ovary. Journal of Physiology, 196:4-5.         [ Links ]

GILBERT, A.B. 1969. Innervation of the ovary of the domestic hen. Quarterly Journal of Experimental Physiology, 54:404-411.         [ Links ]

JANA, B., DZIENIS, A., PANCZYSZYN, J., ROGOZINSKA, A., WOJTKIEWICZ, J., SKOBOWIAT, C. & MAJEWSKI, M. 2005. Denervation of the porcine ovaries performed during the early luteal phase influenced morphology and function of the gonad. Reproductive Biology, 5:69-82.         [ Links ]

KANNISTO, P., OWMAN, C.H., ROSENGREN, E. & WALLES, B. 1984. Intraovarian adrenergic nerves in the guinea-pig: development from fetal life to sexual maturity. Cell and Tissue Research, 238:235-240.         [ Links ]

KIMARO, W.H. & MADEKUROZWA, M-C. 2006. Immunoreactivities to protein gene product 9.5, neurofilament protein and neuron specific enolase in the ovary of the sexually immature ostrich (Struthio camelus). Experimental Brain Research, 173:291-297.         [ Links ]

KOTWICA, J., BOGACKI, M. & REKAWIECKI, R. 2002. Neural regulation of the bovine corpus luteum. Domestic Animal Endocrinology, 23:299-308.         [ Links ]

LAKOMY, M., DOBOSZYNSKA, T. & SZTEYN, S. 1983. Adrenergic nerves in organs of the reproductive system of sexually immature pigs. Anatomischer Anzeiger, 154:149-159.         [ Links ]

LARA, H.E., DORFMAN, M., VENEGAS, M., LUZA, S.M., LUNA, S.L., MAYERHOFER, A., GUIMARAES, M.A., ROSA E SILVA, A.A.M. & RAMIREZ, V.D. 2002. Changes in sympathetic nerve activity of the mammalian ovary during a normal estrous cycle and in polycystic ovary syndrome: studies on norepinephrine release. Microscopy Research and Technique, 59:495-502.         [ Links ]

LAWRENCE, I.E. & BURDEN, H.W. 1980. The origin of the extrinsic adrenergic innervation to the rat ovary. Anatomical Record, 196:51-59.         [ Links ]

LUNA, F., CORTES, M., FLORES, M., HERNANDEZ, B., TRUJILLO, A. & DOMINGUEZ, R. 2003. The effects of superior ovarian nerve sectioning on ovulation in the guinea pig. Reproductive Biology and Endocrinology, 1:61-67.         [ Links ]

MALAMED, S., GIBNEY, J.A. & OJEDA, S.R. 1992. Ovarian innervation develops before initiation of folliculogenesis in the rat. Cell and Tissue Research, 270:87-93.         [ Links ]

MARTIN, R., FRAILE, B., PEINADO, F., ARENAS, M.I., ELICES, M., ALONSO, L., PANIAGUA, R., MARTIN J.J. & SANTA-MARIA, L. 2000. Immunohistochemical localization of protein gene product 9.5, ubiquitin and neuropeptide Y immuno-reactivities in epithelial and neuroendocrine cells from normal and hyperplastic human prostate. Journal of Histochemistry and Cytochemistry, 48:1121-1130.         [ Links ]

MINNAAR, P. & MINNAAR, M. 1997. The emu farmer's handbook. Texas: Nyoni Publishing Co.         [ Links ]

OHARA, O., GAHARA, Y., MIYAKE, T., TERAOKA, H. & KITAMURA, T. 1993. Neurofilament deficiency in quail caused by nonsense mutation in neurofilament-L gene. Journal of Cell Biology, 121:387-395.         [ Links ]

SPORRONG, B., KANNISTO, P., OWMAN, C.H., SJOBERG, N.O. & WALLES, B. 1985. Histochemistry and ultrastructure of adrenergic and acetylcholinesterase-containing nerves supplying follicles and endocrine cells in the guinea-pig ovary. Cell and Tissue Research, 240:505-511.         [ Links ]

THEODOROPOULOS, V.E., TSIGKA, A., MIHALOPOULOU, A., TSOUKALA, V., LAZARIS, A.C., PATSOURIS, E. & GHIKONTI, I. 2005. Evaluation of neuroendocrine staining and androgen receptor expression in incidental prostatic adenocarci-noma: prognostic implications. Urology, 66:897-902.         [ Links ]

UNSICKER, K., SEIDEL, F., HOFMANN, H.D., MULLER, T.H., SCHMIDT, R. & WILSON, A. 1983. Catecholaminergic innervation of the chicken ovary. Cell and Tissue Research, 230:431-450.         [ Links ]

WEISS, G.K., DAIL, W.G. & RATNER, A. 1982. Evidence for direct neural control of ovarian steroidogenesis in rats. Journal of Reproduction and Fertility, 65:507-511.         [ Links ]

 

 

Accepted for publication 10 October 2007-Editor

 

 

* E-mail: madex@op.up.ac.za