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Onderstepoort Journal of Veterinary Research

versão On-line ISSN 2219-0635
versão impressa ISSN 0030-2465

Onderstepoort j. vet. res. vol.75 no.1 Cape Town  2008




Evaluation of different adjuvants for foot-and-mouth disease vaccine containing all the SAT serotypes



M. CloeteI; B. DunguI, II; L.I. Van StadenI; N. Ismail-CassimI; W. VoslooI

ITransboundary Animal Diseases Programme, Onderstepoort Veterinary Institute, Private Bag X05, Onderstepoort, 0110 South Africa
IIOnderstepoort Biological Products, Private Bag X07, Onderstepoort, 0110 South Africa
IIIDepartment of Veterinary Tropical Diseases, University of Pretoria, Pretoria, 0110 South Africa




Foot-and-mouth disease (FMD) is an economically important disease of cloven-hoofed animals that is primarily controlled by vaccination of susceptible animals and movement restrictions for animals and animal-derived products in South Africa. Vaccination using aluminium hydroxide gel-saponin (AS) adjuvanted vaccines containing the South African Territories (SAT) serotypes has been shown to be effective both in ensuring that disease does not spread from the endemic to the free zone and in controlling outbreaks in the free zone. Various vaccine formulations containing antigens derived from the SAT serotypes were tested in cattle that were challenged 1 year later. Both the AS and ISA 206B vaccines adjuvanted with saponin protected cattle against virulent virus challenge. The oil-based ISA 206B-adjuvanted vaccine with and without stimulators was evaluated in a field trial and both elicited antibody responses that lasted for 1 year. Furthermore, the ISA 206 adjuvanted FMD vaccine protected groups of cattle against homologous virus challenge at very low payloads, while pigs vaccinated with an emergency ISA 206B-based FMD vaccine containing the SAT 1 vaccine strains were protected against the heterologous SAT 1 outbreak strain.

Keywords: Adjuvant, cattle, control strategies, foot-and-mouth disease, pigs, South Africa, vaccines



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AUCOUTURIER, J., DUPUIS, L. & GANNE, V. 2001. Adjuvants designed for veterinary and human vaccines. Vaccine, 19: 2666-2672.         [ Links ]

BAHNEMANN, H.G. & MESQUITA, J.A. 1987. Oil-adjuvanted vaccine against foot-and-mouth disease. Bulletin Centro Panamericano Fiebre Altosa, 53:25-50.         [ Links ]

BARNETT, P.V. & CARABIN, H. 2002. A review of emergency foot-and-mouth disease (FMD) vaccines. Vaccine, 20:1505-1514.         [ Links ]

BARNETT, P.V., COX, S.J., AGGARWAL, N., GERBER, H. & MCCULLOUGH, K.C. 2002. Further studies on the early protective responses of pigs following immunization with high potency foot-and-mouth disease vaccine. Vaccine, 20:3197-3208.         [ Links ]

BARNETT, P.V. & DOEL, T.R. 1992. Stability and potency studies of FMD vaccines prepared from antigens stored in the Pirbright bank: comparison between the oil and Al(OH)3 formulations. Report of the Research Group of the Standing Technical Committee of the European Commission for the Control of Foot-and-mouth Disease, Berne.         [ Links ]

BARNETT, P.V., KEEL, P., REID, S., ARMSTRONG, R.M., STATHAM, R.J., VOYCE, C., AGGARWAL, N. & COX, S.J. 2004. Evidence that high potency foot-and-mouth disease vaccine inhibits local virus replication and prevents the carrier state in sheep. Vaccine, 22:1221-1232.         [ Links ]

BARNETT, P.V., PULLEN, L., WILLIAMS, L. & DOEL, T.R. 1996. International bank for foot-and-mouth disease vaccine: assessment of Montanide ISA 25 and ISA 206, two commercially available oil adjuvants. Vaccine, 14:1187-1198.         [ Links ]

BARNETT, P.V. & STATHAM, R.J. 2002. Stratified and cryogenically stored (SACS) vaccines, a new concept in emergency foot-and-mouth disease vaccine formulation and storage. Vaccine, 20:2060-2064.         [ Links ]

BARNETT, P.V., STATHAM, R.J., VOSLOO, W. & HAYDON, D.T. 2003. Foot-and-mouth disease vaccine potency testing: determination and statistical validation of a model using a serological approach. Vaccine, 21:3240-3248.         [ Links ]

BRÜCKNER, G.K., VOSLOO, W., DU PLESSIS, B.J.A., KLOECK, P.E.L.G., CONNOWAY, L., EKRON, M.D., WEAVER, D.B., DICKASON, C.J., SCHREUDER, F.J., MARAIS., T. & MOGAJANE, E. 2002. Foot-and-mouth disease: the experience of South Africa. Revue Scientifique et Technique, 21:751-764.         [ Links ]

BRÜCKNER, G.K., VOSLOO, W., CLOETE, M., DUNGU, B. & DU PLESSIS, B.J. 2004. Foot-and-mouth disease control using vaccination: South African experience. Developmental Biology, 119:51-62.         [ Links ]

CHILDERSTONE, A.J., CEDILLO-BARON, L., FOSTER-CUEVAS, M. & PARKHOUSE, M.E. 1999. Demonstration of bovine CD8+ T-cell responses to foot-and-mouth disease virus. Journal of General Virology, 80:663-669.         [ Links ]

COTTRAL, G.E. 1972a. Foot-and-mouth disease virus neutralization test cross reactions. Bulletin Office International des Epizooties, 77:1239-1261.         [ Links ]

COTTRAL, G.E. 1972b. Foot-and-mouth disease virus neutralization test cross reactions: a comparison of cattle and guinea pig serums. Proceedings of the 76th Annual Meeting of the US Animal Health Association: 194-207.         [ Links ]

COX, S.J., BARNETT, P.V., DANI, P. & SALT, J.S. 1999. Emergency vaccination of sheep against foot-and-mouth disease: protection against disease and reduction in contact transmission. Vaccine, 17:1858-1868.         [ Links ]

COX, J.C. & COULTER, A.R. 1997. Adjuvants-a classification and review of their modes of action. Vaccine, 15:248-256.         [ Links ]

COX, S.J., VOYCE, C., PARIDA, S., REID, S.M., HAMBLIN, P.A., PATON, D. & BARNETT, P.V. 2005. Protection against direct-contact challenge following emergency FMD vaccination of cattle and the effect of virus secretion of the oropharynx. Vaccine, 23:1106-1113.         [ Links ]

COX, S.J., VOYCE, C., PARIDA, S., REID, S.M., HAMBLIN, P.A., HUTCHINGS, D., PATON, D. & BARNETT, P.V. 2006. Effect of emergency FMD vaccine payload on protection, sub-clinical infection and persistence following direct contact challenge of cattle. Vaccine, 24:3184-3190.         [ Links ]

DE LEEUW, P.W., TIESSINK, J.W.A. & VAN BEKKUM, J.G. 1979. The challenge of vaccinated pigs with foot-and-mouth disease virus. Zentralblatt für Veterinärmedizin, 26:98-109.         [ Links ]

DORA, J.F.P., COELHO NUNES, J.C., GOULAR DE SILVEIRA, J.C., JORGENS, E.N., ROSENBERG, J.F. & ASTUDILLO, V.M. 1984. Epidemic of foot-and-and-mouth disease in Bage, R.S., Brazil, 1980: Evaluation of two systems of vaccination. Bulletin Centro Panamericano Fiebre Altosa, 49-50:11-17.         [ Links ]

EBLÉ, P.L., BOUMA, A., DE BRUIN, M.G.M., VAN HEMERT-KLUITENBERG, F., VAN OIRSCHOT, J.T. & DEKKER, A. 2004. Vaccination of pigs two weeks before infection significantly reduces transmission of foot-and-mouth disease virus. Vaccine, 22:1372-1378.         [ Links ]

EBLÉ, P.L., DE BRUIN, M.G.M., BOUMA, A. VAN HEMERT-KLUITENBERG, F. & DEKKER, A. 2006. Comparison of immune responses after intra-typic heterologous and homologous vaccination against foot-and-mouth disease virus infection in pigs. Vaccine, 24:1274-1281.         [ Links ]

EBLÉ, P.L., BOUMA, A., WEERDMEESTER, K., STEGEMAN, J.A., & DEKKER, A. 2007. Serological and mucosal immune responses after vaccination and infection with FMDV in pigs. Vaccine, 25:1043-1054.         [ Links ]

GOLDE, W.T., PACHECO, J.M., DUQUE, H., DOEL, T., PEN-FOLD, B., FERMAN, G.S., GREGG, D.R. & RODRIQUEZ, L.L. 2005. Vaccination against foot-and-mouth disease confers complete protection in 7 days and partial protection in 4 days: Use in emergency outbreak response. Vaccine, 23: 5775-5782.         [ Links ]

HAMBLIN, C., BARNETT, I.T. & HEDGER, R.S.A. 1986. A new enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus. I. Development and method of ELISA. Journal of Immunological Methods, 93:115-121.         [ Links ]

HUNTER, P. 1996. The performance of southern African territories serotypes of foot-and-mouth disease antigen in oil-adjuvanted vaccines. Revue Scientifique et Technique, 15:913-922.         [ Links ]

HUNTER, P. 1998. Vaccination as a means of control of foot-and-mouth disease in sub-Saharan Africa. Vaccine, 16:261-264.         [ Links ]

IYER, A.V., GHOSH, S., SINGH, S.V. & DESHMUKH, R.A. 2001. Evaluation of three ready to formulate oil adjuvants for foot-and-mouth disease vaccine production. Vaccine, 19:1097-1105.         [ Links ]

KITCHING, R.P., BARNETT, P.V. & DONALDSON, A.I. 2000. Foot-and-mouth disease, in Manual of standards for diagnostic tests and vaccines, edited by OIE Biological Standards Committee. Paris: OIE        [ Links ]

PATIL, P.K., BAYRY, J., RAMAKRISHNA, C., HUGAR, B., MISRA, L.D. & NATARAJAN, C. 2002a. Immune responses of goats to foot-and-mouth disease quadrivalent vaccine: comparison of double-oil emulsion and aluminium hydroxide gel vaccines in eliciting immunity. Vaccine, 20:2781-2789.         [ Links ]

PATIL, P.K., BAYRY, J., RAMAKRISHNA, C., HUGAR, B., MISRA, L.D., PRABHUDAS, K. & NATARAJAN, C. 2002b. Immune responses of sheep to foot-and-mouth disease quadrivalent vaccine: rate of development of immunity and variations among other ruminants. Journal of Clinical Microbiology, 40:4367-4371.         [ Links ]

PAYNE, R.W. (Ed). 2003. Genstat® for Windows® , 7th ed. (Introduction). VSA International.         [ Links ]

QUATTROCCHI, V., BIANCO, V., FONDEVILA, N., PAPPALARDO, S., SADIRA, A. & ZAMORANO, P. 2004. Use of new adjuvants in an emergency vaccine against foot-and-mouth disease virus: evaluation of conferred immunity. Control of Infectious Animal Diseases by Vaccination, Developmental Biology, 119:481-497.         [ Links ]

QUATTROCCHI, V., FONDEVILA, N., PAPPALARDO, S., SADIRA, A. & ZAMORANO, P. 2006. Development of an adjuvanted vaccine with rapid delivery against foot-and-mouth disease virus (FMDV). The Third International Workshop on Vaccine Adjuvants and Glycoconjugates, Varadero, Cuba, April 16-20, 2006.         [ Links ]

RWEYEMAMU, M.M., BOOTH, J.C., HEAD, M. & PAY, T.W.F. 1978. Microneutralization tests for serological typing and subtyping of foot-and-mouth disease virus strains. Journal of Hygiene (London), 81:107-123.         [ Links ]

SMITSAART, E., ESPONOZA, A.M., SANGUINETTI, R., FILIPPI, J., HAM, A. & BELLINZONI, R. 2004. Addition of saponin to double oil emulsion FMD vaccines enhances specific antibody responses in cattle and pigs. Report on the European Commission for the control of foot-and-mouth disease. Session of the Research Group of the Technical Standing Committee, Food and Agriculture Organization of the United Nations, Chania, Greece, 11-15 October 2004: 344-351.         [ Links ]

SMITSAART, E., MATTION, N., FILIPPI, J.L., ROBIOLO, B., PERIOLO, O., LA TORRE, J. & BELLINZONI, R.C. 2000. Enhancement of the immune response induced by the inclusion of saponin in oil adjuvanted vaccines against foot-and-mouth disease. Session of the Research Group of the Standing Technical Committee of the European Commission for the Control of FMD. Borovets, Bulgaria (Appendix), 32:255-262.         [ Links ]

SNEDECOR, G.W. & COCHRAN, W.G. 1980. Statistical methods, 7th ed. Ames: Iowa State University Press.         [ Links ]

STELLMANN, C. TERR, J., FAVRE, H., BRUN, A. & FONTAINE, J. 1977. Comparison of foot-and-mouth disease vaccine potency in terms of the nature of the diluent. Archives of Virology, 54:61-74.         [ Links ]

SUTMOLLER, P., BARTELING, S.S., OLASCOAGA, R.C. & SUMPTION, K.J. 2003. Control and eradication of foot-and-mouth disease. Virus Research, 91:10-144.         [ Links ]

VAN BEKKUM, J.G., BOOL, P.H. & VERMEULEN, C.J. 1967. Experience with the vaccination of pigs for the control of foot-and-mouth disease in the Netherlands. Tijdschrift voor Diergeneeskunde, 92:87-97.         [ Links ]

VOSLOO, W., BASTOS, A.D.S., SANGARE, O., HARGREAVES, S.K. & THOMSON, G.R. 2002a. Review of the status and control of foot-and-mouth disease in sub-Saharan Africa. Revue Scientifique et Technique, 21:437-449.         [ Links ]

VOSLOO, W., BOSHOFF, K., DWARKA, R. & BASTOS, A. 2002b. The possible role that buffalo played in the recent outbreaks of foot-and-mouth disease in South Africa. Annals of the New York Academy of Sciences, 969:187-190.         [ Links ]



Accepted for publication 22 October 2007-Editor



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