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Onderstepoort Journal of Veterinary Research

On-line version ISSN 2219-0635
Print version ISSN 0030-2465

Onderstepoort j. vet. res. vol.75 n.1 Pretoria  2008




Evaluation of different adjuvants for foot-and-mouth disease vaccine containing all the SAT serotypes



M. CloeteI; B. DunguI, II; L.I. Van StadenI; N. Ismail-CassimI; W. VoslooI

ITransboundary Animal Diseases Programme, Onderstepoort Veterinary Institute, Private Bag X05, Onderstepoort, 0110 South Africa
IIOnderstepoort Biological Products, Private Bag X07, Onderstepoort, 0110 South Africa
IIIDepartment of Veterinary Tropical Diseases, University of Pretoria, Pretoria, 0110 South Africa




Foot-and-mouth disease (FMD) is an economically important disease of cloven-hoofed animals that is primarily controlled by vaccination of susceptible animals and movement restrictions for animals and animal-derived products in South Africa. Vaccination using aluminium hydroxide gel-saponin (AS) adjuvanted vaccines containing the South African Territories (SAT) serotypes has been shown to be effective both in ensuring that disease does not spread from the endemic to the free zone and in controlling outbreaks in the free zone. Various vaccine formulations containing antigens derived from the SAT serotypes were tested in cattle that were challenged 1 year later. Both the AS and ISA 206B vaccines adjuvanted with saponin protected cattle against virulent virus challenge. The oil-based ISA 206B-adjuvanted vaccine with and without stimulators was evaluated in a field trial and both elicited antibody responses that lasted for 1 year. Furthermore, the ISA 206 adjuvanted FMD vaccine protected groups of cattle against homologous virus challenge at very low payloads, while pigs vaccinated with an emergency ISA 206B-based FMD vaccine containing the SAT 1 vaccine strains were protected against the heterologous SAT 1 outbreak strain.

Keywords: Adjuvant, cattle, control strategies, foot-and-mouth disease, pigs, South Africa, vaccines



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Accepted for publication 22 October 2007-Editor



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