Abstract

FADANA, Vuyolwethu; THOMAS, Teena  and  VON KNORRING, Nina. Retrospective analysis of Vitek®2 performance compared to manual broth micro-dilution for colistin susceptibility testing of Acinetobacter baumanniicomplex isolates in South Africa. Afr. J. Lab. Med. [online]. 2022, vol.11, n.1, pp.1-4. ISSN 2225-2010.  http://dx.doi.org/10.4102/ajlm.v11i1.1597.

The manual broth micro-dilution (mBMD) is the recommended reference method for colistin minimum inhibitory concentration determination; however, it is not as readily available in South Africa as the Vitek^®2. This retrospective study compared the performance of Vitek^®2 against mBMD in determining the colistin minimum inhibitory concentration of 337 extensively drug-resistant Acinetobacter baumannii complex isolates. Vitek^®2 yielded a categorical agreement of 89%, an essential agreement of 56%, a major error rate of 8% and a very major error rate of 55%. The Vitek^®2 is not an alternative to mBMD for colistin susceptibility testing.

Keywords : Acinetobacter; colistin; broth micro-dilution; Vitek^®2; antimicrobial susceptibility testing.

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