Abstract

SENAMELA, Tshiphiri; KOCK, Marleen; BECKER, Piet  and  POTGIETER, Joachim J.C.. Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay. Afr. J. Lab. Med. [online]. 2018, vol.7, n.1, pp.1-4. ISSN 2225-2010.  http://dx.doi.org/10.4102/ajlm.v7i1.658.

The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation.

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