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African Journal of Laboratory Medicine
versão On-line ISSN 2225-2010
versão impressa ISSN 2225-2002
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MAHOMED, Sharana; DLAMINI-MVELASE, Nomonde R.; DLAMINI, Moses e MLISANA, Koleka. Failure of BACTEC™ MGIT 960™ to detect Mycobacterium tuberculosis complex within a 42-day incubation period. Afr. J. Lab. Med. [online]. 2017, vol.6, n.1, pp.1-3. ISSN 2225-2010. http://dx.doi.org/10.4102/ajlm.v6i1.537.
For the optimal recovery of Mycobacterium tuberculosis from the BACTEC™ Mycobacterium Growth Indicator Tube 960™ system, an incubation period of 42-56 days is recommended by the manufacturer. Due to logistical reasons, it is common practice to follow an incubation period of 42 days. We undertook a retrospective study to document positive Mycobacterium Growth Indicator Tube cultures beyond the 42-day incubation period. In total, 98/110 (89%) were positive for M. tuberculosis complex. This alerted us to M. tuberculosis growth detection failure at 42 days.