SciELO - Scientific Electronic Library Online

vol.42 issue3First report on the colony-forming freshwater ciliate Ophrydium versatile in an African riverHistorical trends in the flows of the Breede River author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand



Related links

  • On index processCited by Google
  • On index processSimilars in Google


Water SA

On-line version ISSN 1816-7950
Print version ISSN 0378-4738


VAN DEN BERG, MF; BOTES, M; SLABBERT, E  and  CLOETE, TE. Evaluating sulphate removal and identifying the bacterial community present in acid mine drainage treated with synthetic domestic wastewater sludge. Water SA [online]. 2016, vol.42, n.3, pp.475-482. ISSN 1816-7950.

Domestic wastewater sludge can serve as a carbon source in the passive biotic treatment of acid mine drainage (AMD) in microbial bioreactors to create anaerobic conditions for the removal of sulphate, chemical oxygen demand (COD) and pH neutralization. A synthetic medium simulating domestic wastewater sludge was used in AMD treatment in a ratio of 1:1 AMD: synthetic domestic wastewater sludge (SDWWS). Sulphate and COD removal were determined at different incubation temperatures and with and without a biofilm in the bioreactors. Sulphate and COD were removed by 60.8% and 96% within 26 d, after which a plateau was reached. Bacterial community analyses using next generation sequencing showed that Chlorobium spp. dominated at a relative percentage of 36% followed by Magnetospirillum spp. and Ornithobacterium spp. The effect of a resident biofilm in the bioreactors showed dominance of Chlorobium spp. at a relative percentage of 62% and removal of sulphates and COD at 96% and 58%, respectively, after 26 d. Incubation at 17-19°C reduced sulphates by only 10% and COD by 12% after 17 d, after which a plateau was reached. Magnetospirillum spp. was the dominate organism at the end of this trial.

Keywords : acid mine drainage; domestic wastewater sludge; sulphates; COD; bacterial community.

        · text in English     · English ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License