SciELO - Scientific Electronic Library Online

 
vol.38 issue5Use of calcium sulphate dihydrate as an alternative to the conventional use of aluminium sulphate in the primary treatment of wastewaterReduction of Langelier index of cooling water by electrolytic treatment with stainless steel electrode author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Water SA

On-line version ISSN 0378-4738

Abstract

PAULSE, AN; JACKSON, VA; KHAN, S  and  KHAN, W. Isolation and identification of bacterial pollutants from the Berg and Plankenburg Rivers in the Western Cape, South Africa. Water SA [online]. 2012, vol.38, n.5, pp. 819-824. ISSN 0378-4738.

Bacterial species present in the Berg and Plankenburg Rivers (Western Cape, South Africa) were isolated from water and biofilm samples and population shifts between sampling sites were phylogenetically identified. Deoxyribonucleic acid (DNA) extraction of representative isolates was performed and amplified using 2 different primer sets. Various Enterobacteriaceae species were present at all of the sites, confirming faecal contamination. Phylogenetic analyses also showed that, in general, Gram-negative micro-organisms dominated at all of the sites sampled in both the Berg and Plankenburg river systems. Pathogens and opportunistic pathogens, such as Pseudomonas aeruginosa, Staphylococcus sp., and Bacillus cereus, were isolated from the Berg River. Similarly, in the Plankenburg River system, Aeromonas sp., Acinetobacter sp., Stenotrophomonas sp. and Yersinia enterocolitica were also isolated. This raises major health concerns as human population densities along both rivers are high, thus resulting in increased human exposure to these organisms.

Keywords : Enterobacteriaceae; faecal contamination; informal settlement; river water; waterborne illnesses.

        · text in English     · English ( pdf )