Journal of the South African Veterinary Association
versão On-line ISSN 2224-9435
versão impressa ISSN 1019-9128
MULABBI, Elijah N et al. Circulation of bluetongue virus in goats in the Karamoja region of Uganda. J. S. Afr. Vet. Assoc. [online]. 2013, vol.84, n.1, pp.00-00. ISSN 2224-9435.
The presence of bluetongue virus (BTV) in indigenous goats from the Karamoja region of northern Uganda was investigated. A total of 300 goats were sampled (serum and whole blood) from five districts within the Karamoja region. The samples were analysed for the presence of bluetongue (BT) antibodies using a commercial Enzyme-linked immunosorbent assay (ELISA) and for the presence of BTV viral RNA by real-time Reverse transcription polymerase chain reaction (RT-PCR), because BTV is an RNA virus. Of the 300 goats tested, 269 (90%) were positive for BTV antibodies, indicating high levels of BTV circulation within the region. Out of the 150 whole blood samples tested for the presence of the virus by realtime RT-PCR, 84 (56%) were positive for BTV RNA. This study, which is the first of its kind in Uganda, showed a high seroprevalence of BT antibodies and active circulation of BTV in a high proportion of goats in the Karamoja region.