Abstract

AMLASHI, Hamoon Sami; DARYASARI, Ameneh Porgham  and  SOLEIMANI, Mojtaba. Molecularly Imprinted Polymer Solid Phase Extraction followed by High-Performance Liquid Chromatography as an Efficient and Sensitive Technique for Determination of Meropenem in Human Plasma and Urine. S.Afr.j.chem. (Online) [online]. 2019, vol.72, pp.32-39. ISSN 1996-840X.  http://dx.doi.org/10.17159/0379-4350/2019/v72a5.

A new and selective sorbent for molecularly imprinted polymer solid-phase extraction (MIP-SPE) was prepared to extract meropenem from plasma and urine samples. The extracted analyte was analyzed by high-performance liquid chromatography (HPLC) coupled with photodiode array detection. Methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linker, azobisisobutyronitrile (AIBN) as an initiator and meropenem as a template molecule were used for the MIP preparation. Imprinted meropenem molecule was removed from the polymeric structure using acetonitrile in water (1:9, v/v), as the eluent solvent. Under the optimized conditions, the limit of detection (LOD) and limit of quantification (LOQ) were 35 and 120μg L^-1, respectively. The developed MIP-SPE method demonstrates that it could be applied for the determination of meropenem in plasma and urine samples.

Keywords : Molecularly imprinted polymer; solid-phase extraction; meropenem; human urine and plasma; high-performance liquid chromatography.

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