Abstract

LIN, C.W. et al. Effects of polymorphisms in the endothelin receptor B subtype 2 gene on plumage colour in mule ducks. S. Afr. j. anim. sci. [online]. 2020, vol.50, n.2, pp.310-317. ISSN 2221-4062.  http://dx.doi.org/10.4314/sajas.v50i2.14.

The aim of the present study was to investigate the effect of a single nucleotide polymorphism (SNP) of the endothelin receptor B subtype 2 (EDNRB2) gene on plumage coloration in mule ducks. Test mating (white Tsaiya * white Muscovy ducks) in combination with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to investigate the effect of non-synonymous SNPs in two maternal lines (a conservation and a selection population) on plumage coloration in mule ducks. One non-synonymous SNP (c.995G>A) was identified in white Muscovy ducks and white Tsaiya ducks by PCR-high-resolution melting (PCR-HRM) and DNA sequencing. Genotyping results showed that the c.995G>A locus is associated with plumage colour in two maternal populations of white Tsaiya ducks. Further, the maternal genotype of c.995G>A SNP affects the plumage colour of mule ducks. Therefore, the polymorphisms within the EDNRB2 gene at c.995G>A in white Tsaiya ducks may be used in marker-assisted selection to improve the plumage colour of mule ducks.

Keywords : Muscovy drakes; polymerase chain reaction-restriction fragment length; single nucleotide polymorphism; Tsaiya ducks.

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