SciELO - Scientific Electronic Library Online

vol.47 número2Evaluation of growth, milk and manure production in Norwegian dairy goats in one highland of Tanzania 30 years after introductionPalm kernel expeller increases milk fat content when fed to grazing dairy cows índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados



Links relacionados

  • Em processo de indexaçãoCitado por Google
  • Em processo de indexaçãoSimilares em Google


South African Journal of Animal Science

versão On-line ISSN 2221-4062
versão impressa ISSN 0375-1589


FRASER, L.; STRZEżEK, J.; WASILEWSKA, K.  e  PAREEK, C.S.. Sperm DNA damage in relation to lipid peroxidation following freezing-thawing of boar semen. S. Afr. j. anim. sci. [online]. 2017, vol.47, n.2, pp.213-218. ISSN 2221-4062.

This study investigated the relationships between lipid peroxidation (LPO) and sperm DNA damage following freezing-thawing of boar semen in different extenders. The comet assay was used to measure the extent of sperm DNA damage in a cryoprotectant-free extender or in cryoprotectant-based extenders after single and repeated freezing and thawing. As well as an analysis of sperm motion characteristics, mitochondrial function, membrane integrity, and lipid peroxidation (LPO) were assessed simultaneously with the measurements of sperm DNA damage. Consistent positive significant correlations were found between sperm DNA damage and LPO after freezing-thawing. Comet assay measurements showed that cryo-induced sperm DNA damage was more marked in the cryoprotectant-free extender, irrespective of freezing cycle. The frequency of sperm cells with damaged DNA increased with repeated freezing and thawing in the cryoprotectant-based extenders. Except for sperm DNA damage, there were no consistent associations between post-thaw sperm LPO and sperm quality characteristics. It could be suggested that the increased LPO of membrane phospholipids is associated with higher susceptibility of boar spermatozoa to cryo-induced DNA damage.

Palavras-chave : Comet assay measurements; cryopreservation; extenders; spermatozoa.

        · texto em Inglês     · Inglês ( pdf )


Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons