SciELO - Scientific Electronic Library Online

 
vol.45 issue2Diallel analysis for bodyweight involving three genotypes of Nigerian indigenous chickensPotential enhancing effects of a carbohydrase mixture on phytase efficacy in late-phase laying hens fed wheat-based diets author indexsubject indexarticles search
Home Pagealphabetic serial listing  

South African Journal of Animal Science

On-line version ISSN 2221-4062
Print version ISSN 0375-1589

Abstract

SHARMA, R. et al. First-time characterization of JY-1-like sequence in goats. S. Afr. j. anim. sci. [online]. 2015, vol.45, n.2, pp.198-205. ISSN 2221-4062.  http://dx.doi.org/10.4314/SAJAS.V45I2.11.

The JY-1 protein is oocyte specific, and is associated with folliculogenesis and early embryo development, and thus influences the chance of pregnancy. It was the first protein of maternal origin to be described for a single ovulating species, namely cattle. JY-1-like sequences corresponding to 3' coding and the untranslated region have been reported in other vertebrate species. This is the first description of the partial JY-1 (exon 3 and 3'UTR) in a livestock species other than cattle and buffalo. The sequence was characterized in a panel of nine Indian goat breeds, which differ in reproductive traits (twinning percentage and age of sexual maturity). Forty three variations were recorded in the analysed region of goats JY-1 compared with cattle. Nucleotide variations in the codifying region of goats correspond to seven amino acid changes that could affect the biological function of the protein and possibly reproductive differences between goats (higher proportion of multiparous animals) and cattle (mainly uniparous). A novel single nucleotide polymorphism (C15329T) has been identified in Indian goats, which was genotyped in 272 animals from six breeds. Further studies to investigate other regions of the gene and its expression in goat female reproductive tissues would clarify the role of JY-1 in farm animal species that are not primarily uniparous.

Keywords : Capra hircus; embryogenesis; fertility; folliculogenesis; oocyte; PCR-RFLP; polymorphism; SNP.

        · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License