SciELO - Scientific Electronic Library Online

 
vol.43 número4Genetic relationship between growth and carcass traits in Large White pigsAssessing the genetic diversity of five Tanzanian chicken ecotypes using molecular tools índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Articulo

Indicadores

Links relacionados

  • En proceso de indezaciónCitado por Google
  • En proceso de indezaciónSimilares en Google

Compartir


South African Journal of Animal Science

versión On-line ISSN 2221-4062

Resumen

NOORI, R. et al. Association of polymorphism in Exon 3 of toll-like receptor 4 gene with somatic cell score and milk production traits in Holstein dairy cows of Iran. S. Afr. j. anim. sci. [online]. 2013, vol.43, n.4, pp. 493-498. ISSN 2221-4062.

Mastitis is a complex inflammatory disease of the mammary gland that is caused by the invasion of pathogens. This leads to reduced synthetic capacity, compositional changes and increased somatic cell counts (SCC) in milk. Toll-like receptor 4 (TLR4) is a cell surface receptor that recognizes lipopolysaccharides (LPS) of gram-negative bacteria. Its role in pathogen recognition and the subsequent immune response and differential expression of the gene during mastitis have prompted the investigation of TLR4 gene as a candidate to improve mastitis resistance in dairy cattle. The aim of this study was to analyse the possible association of a single nucleotide polymorphism (SNP) in putative co-receptor-binding region 2 (T4CRBR2) of the TLR4 gene with somatic cell score (SCS) and milk-related traits in 408 Iranian Holstein cows. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was performed for genotyping. The effect of the genotype on the traits of interest was analysed using the general linear model (GLM) procedure of SAS software. The B allele frequency was 0.634 and the distribution of genotypes was not in Hardy-Weinberg equilibrium in the overall population. The B allele of the SNP was associated with higher 305-day milk yield and breeding value for milk yield, and lower fat percentage and lower SCS, as compared with allele A. The association between TLR4 polymorphism with SCS and milk production traits reported in this study suggests that this SNP has the potential to be used as a marker for selection, based on molecular information.

Palabras clave : TLR4 gene; mastitis; candidate gene; milk-related traits; single nucleotide polymorphism; SNP.

        · texto en Inglés     · Inglés ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License