SciELO - Scientific Electronic Library Online

 
vol.105 issue4Utility of the Robson Ten Group Classification System to determine appropriateness of caesarean section at a rural regional hospital in KwaZulu-Natal, South AfricaIntrapartum asphyxia and hypoxic ischaemic encephalopathy in a public hospital: incidence and predictors of poor outcome author indexsubject indexarticles search
Home Pagealphabetic serial listing  

SAMJ: South African Medical Journal

On-line version ISSN 2078-5135

Abstract

FRANKEN, D R. Office-based sperm concentration: a simplified method for intrauterine insemination therapy. SAMJ, S. Afr. med. j. [online]. 2015, vol.105, n.4, pp. 295-297. ISSN 2078-5135.  http://dx.doi.org/10.7196/SAMJ.8944.

BACKGROUND: Intrauterine insemination (IUI) could become preferred to more invasive and expensive techniques of assisted reproduction therapy (ART) and should be offered as the first choice in cases with no female factors and mild male factor subfertility. However, developing countries and especially their rural areas often lack the necessary equipment and laboratory facilities OBJECTIVE: To describe a simplified one-step method to determine the sperm concentration range for IUI therapy METHODS: Semen samples from 51 sperm donors were used. Following swim-up separation, the sperm concentration of the retrieved motile fraction was counted, as well as progressive motile sperm using a standardised wet preparation. The number of sperm in a 10 μl droplet covered with a 22 χ 22 mm coverslip was counted under 400 χ total magnification. The observed numbers of retrieved motile sperm were divided into three groups: <40, 40 - 100 and >101 spermatozoa as recorded per intial estimation on the wet preparation RESULTS: The mean (standard deviation) estimated sperm concentration for each group compared with actual counts per Neubauer counting chamber were: estimated <40 sperm (n=14), mean 20 (8), Neubauer count 2.5 x 106/mL; estimated 40 - 100 sperm (n=14), mean 71 (15), Neubauer count 16 x 106/mL; and estimated >100 sperm (n=23), Neubauer count 48.3 (21.7) χ 106/mL CONCLUSION: The results with IUI in male subfertility cases reported by Ombelet et al. in 1995 support the concept of first-line treatment of infertility by three to four cycles of IUI therapy in selected cases

        · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License