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South African Journal of Science
On-line version ISSN 1996-7489
Print version ISSN 0038-2353
Abstract
LEPHOTO, Tiisetso E. and GRAY, Vincent M.. First report of the isolation of entomopathogenic nematode Steinernema australe (Rhabditida: Steinernematidae) from South Africa. S. Afr. j. sci. [online]. 2019, vol.115, n.11-12, pp.1-5. ISSN 1996-7489. http://dx.doi.org/10.17159/sajs.2019/6008.
A survey was conducted in Walkerville, south of Johannesburg (Gauteng, South Africa) between 2012 and 2016 to ascertain the diversity of entomopathogenic nematodes in the area. Entomopathogenic nematodes are soil-dwelling microscopic worms with the ability to infect and kill insects, and thus serve as eco-friendly control agents for problem insects in agriculture. Steinernematids were recovered in 1 out of 80 soil samples from uncultivated grassland; soil was characterised as loamy. The entomopathogenic nematodes were identified using molecular and morphological techniques. The isolate was identified as Steinernema australe. This report is the first of Steinernema australe in South Africa. S. australe was first isolated worldwide from a soil sample obtained from the beach on Isla Magdalena - an island in the Pacific Ocean, 2 km from mainland Chile.SIGNIFICANCE: •Entomopathogenic nematodes are only parasitic to insects and are therefore important in agriculture as they can serve as eco-friendly biopesticides to control problem insects without effects on the environment, humans and other animals, unlike chemical pesticides
Keywords : strain; morphological variations; survey; biocontrol agent.