South African Journal of Science
On-line version ISSN 1996-7489
Print version ISSN 0038-2353
MEYER, Patrícia Froes et al. Cellular and molecular effects of electromagnetic radiation and sonic waves. S. Afr. j. sci. [online]. 2013, vol.109, n.7-8, pp.01-04. ISSN 1996-7489.
Electromagnetic radiation (in the form of pulsed magnetic fields, radiofrequency and intense pulsed light) and mechanical agents (such as sonic waves) have been used in physical therapy. The aim of this study was to assess the effects of low-intensity magnetic fields, sonic and radiofrequency waves, and intense pulsed light on the survival of Escherichia coli cultures and on the electrophoretic mobility of plasmid DNA. Exponentially growing E. coli AB1157 cultures and plasmid DNA samples were exposed to these physical agents and 0.9% NaCl (negative control) and SnCl2 (positive control) solutions. Aliquots of the cultures were diluted and spread onto a solidified rich medium. The colony-forming units were counted after overnight incubation and the survival fraction was calculated. Agarose gel electrophoresis was performed to visualise and quantify the plasmid topological forms. The results suggest that these agents do not alter the survival of E. coli cells or plasmid DNA electrophoresis mobility. Moreover, they do not protect against the lesive action of SnCl2. These physical agents therefore had no cytotoxic or genotoxic effects under the conditions studied.
Keywords : Escherichia coli; DNA; intense pulsed light; magnetic field; radiofrequency; tannous chloride.