South African Journal of Science
On-line version ISSN 1996-7489
Print version ISSN 0038-2353
MUSINGARIMI, Wicleffe; TUFFIN, Marla and COWAN, Donald. Characterisation of the arsenic resistance genes in Bacillus sp. UWC isolated from maturing fly ash acid mine drainage neutralised solids. S. Afr. j. sci. [online]. 2010, vol.106, n.1-2, pp.59-63. ISSN 1996-7489.
An arsenic resistant Bacillus sp. UWC was isolated from fly ash acid mine drainage (FA-AMD) neutralised solids. A genomic library was prepared and screened in an arsenic sensitive mutant Escherichia coli strain for the presence of arsenic resistance (ars) genes. Sequence analysis of a clone conferring resistance to both sodium arsenite and sodium arsenate revealed homologues to the arsR (regulatory repressor), arsB (membrane located arsenite pump), arsC (arsenate reductase), arsD (second regulatory repressor and a metallochaperone) and arsA (ATPase) genes from known arsenic resistance operons. The Bacillus sp. UWC arsRBCDA genes were shown to be arranged in an unusual manner with the arsDA genes immediately downstream of arsC.
Keywords : fly ash; acid mine drainage; arsenic resistance genes; Bacillus; groundwater contamination.