South African Journal of Science
On-line version ISSN 1996-7489
ADEBOLA, P.O. and AFOLAYAN, A.J.. Plant regeneration from seed-derived callus of Arctotis arctotoides, a medicinal herb of the family Asteraceae. S. Afr. j. sci. [online]. 2007, vol.103, n.3-4, pp. 120-122. ISSN 1996-7489.
We have devised a procedure for in vitro plant regeneration, from seed-derived callus, of Arctotis arctotoides, a plant noted for its medicinal uses among the rural people of the Eastern Cape province of South Africa. Callus formation was induced in basal MS salt supplemented with 3% sucrose and 1% agar at different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D). After two weeks, morphogenic responses were evident in cultures and deeply stained, light-yellow calluses were produced. The highest percentage (68%) of seed explants that induced callus formation was obtained on MS medium supplemented with 2.0 mg l-12,4-D in the dark. When callus material was repeatedly subcultured in ½MS basal medium with a 16-h photoperiod without any hormonal supplement, adventitious shoots were produced eight weeks after the start of culture. The shoots rooted two weeks later. This is the first record of the in vitro regeneration of A arctotoides and could serve as a foundation for further research on tissue culture, micropropagation and germplasm conservation of this noteworthy medicinal plant.