Abstract

BAKHSHI, Fatemeh; LANGROUDI, Reza Pilehchian  and  EIMANI, Bahram Golestani. Enhanced expression of recombinant beta toxin of Clostridium perfringens type B using a commercially available Escherichia coli strain. Onderstepoort j. vet. res. [online]. 2016, vol.83, n.1, pp.1-4. ISSN 2219-0635.  http://dx.doi.org/10.4102/ojvr.v83i1.1136.

Clostridium perfringens beta toxin is only produced by types B and C and plays an important role in many human and animal diseases, causing fatal conditions that originate in the intestines. We compared the expression of C. perfringens type B vaccine strain recombinant beta toxin gene in theEscherichia coli strains Rosetta^TM(DE3) and BL21(DE3). The beta toxin gene was extracted from pJETβ and ligated with pET22b(+). pET22β was transformed into E. coli strains BL21(DE3) and Rosetta^TM(DE3). Recombinant protein was expressed as a soluble protein after isopropyl β-D-1-thiogalactopyranoside (IPTG) induction in strain Rosetta^TM(DE3) but not in BL21(DE3). Expression was optimised by growing recombinant cells at 37 °C and at an induction of 0.5 mM, 1 mM, 1.5 mM IPTG. Expression was evaluated using sodium dodecyl sulfate Polyacrylamide gel electrophoresis (SDS-PAGE). The recombinant protein was purified via Ni-NTA and was analysed using western blot. We concluded that E. coli strain Rosetta^TM(DE3) can enhance the expression of C. perfringens recombinant beta toxin.

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