SciELO - Scientific Electronic Library Online

 
vol.75 número32-Phenoxyethanol as anaesthetic in removing and relocating 102 species of fishes representing 30 families from Sea World to uShaka Marine World, South AfricaEvaluation of biochemical and ultrasonographic measurements as indicators of undernutrition in cattle índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Artigo

Indicadores

Links relacionados

  • Em processo de indexaçãoCitado por Google
  • Em processo de indexaçãoSimilares em Google

Compartilhar


Onderstepoort Journal of Veterinary Research

versão On-line ISSN 2219-0635

Resumo

NGERANWA, J.J.N. et al. Detection of Anaplasma antibodies in wildlife and domestic species in wildlife-livestock interface areas of Kenya by major surface protein 5 competitive inhibition enzyme-linked immunosorbent assay. Onderstepoort j. vet. res. [online]. 2008, vol.75, n.3, pp. 199-205. ISSN 2219-0635.

The seroprevalence of Anaplasma antibodies in wildlife (eland, blue wildebeest, kongoni, impala, Thomson's gazelle, Grant's gazelle, giraffe and plains zebra) and domestic animal (cattle, sheep and goat) populations was studied in wildlife/livestock interface areas of Kenya. Serum samples were analyzed by competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA), using a recombinant antigen (MSP-5) from Anaplasma marginale surface membrane. A monoclonal antibody, FC-16, was used as the primary antibody, while anti-mouse conjugated to horseradish peroxidase was used as the secondary antibody. The results indicate a high seroprevalence in both wildlife and livestock populations, in contrast to earlier reports from Kenya, which indicated a low seroprevalence. The differences are attributed to the accurate analytical method used (CI-ELISA), as compared with agglutination techniques, clinical signs and microscopy employed by the earlier workers.

Palavras-chave : Anaplasma; CI-ELISA; Kenya; seroprevalence; wildlife-livestock interface.

        · texto em Inglês     · Inglês ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License