Onderstepoort Journal of Veterinary Research
On-line version ISSN 2219-0635
Print version ISSN 0030-2465
BARNARD, T.G.; VAN HEERDEN, E.; BRAGG, R.R. and ALBERTYN, J.. Haemophilus paragallinarum haemagglutinin: Role in adhesion, serotyping and pathogenicity. Onderstepoort j. vet. res. [online]. 2008, vol.75, n.1, pp.11-16. ISSN 2219-0635.
It is suggested that Haemophilus paragallinarum requires at least three haemagglutinins for adhesion during infection. This paper reports the partial purification and characterization of the HA-L haemagglutinin from H. paragallinarum strain 46-C3, a heat sensitive, trypsin sensitive haemagglutinin that has been shown to be the serovar specific haemagglutinin in this organism. Using the pi and molecular mass obtained, it was shown that this protein shares similarities with other types of adhesins found in Gram-negative bacteria. The haemagglutination assay conditions were optimized at pH 7.5 at 37 °C. It was also shown that activity is enhanced by the addition of Ca2+ and Mn2+ ions.
Keywords : Haemagglutinin purification and characterization; Haemophilus paragallinarum.