Servicios Personalizados
Articulo
Indicadores
Links relacionados
- Citado por Google
- Similares en Google
Compartir
Onderstepoort Journal of Veterinary Research
versión On-line ISSN 2219-0635
versión impresa ISSN 0030-2465
Resumen
ZWEYGARTH, E.; JOSEMANS, A.I. y STEYN, H.C.. In vitro isolation of Ehrlichia ruminantium from ovine blood into Ixodes scapularis (IDE8) cell cultures. Onderstepoort j. vet. res. [online]. 2008, vol.75, n.2, pp.121-126. ISSN 2219-0635.
Four stocks of Ehrlichia ruminantium (Welgevonden, Ball3, Nonile and Blaauwkrans), the causative agent of heartwater in domestic ruminants, were isolated into Ixodes scapularis (IDE8) tick cells using the leukocyte fraction of the blood of infected sheep. Organisms of two of the E. ruminantium stocks (Welgevonden and Blaauwkrans) propagated in IDE8 cells were also successfully used to infect bovine endothelial cells. All stocks were successfully propagated in IDE8 cells using Dulbecco's modified Eagle's medium nutrient mixture Ham F-12 containing 10 % foetal bovine serum (FBS). The technique should be included in any attempt to isolate uncharacterized E. ruminantium stocks.
Palabras clave : Ehrlichia ruminantium; heartwater; in vitro isolation; tick cell line.