SciELO - Scientific Electronic Library Online

 
vol.75 issue2Bovine intestinal cellular responses following primary and challenge infections with Calicophoron microbothrium metacercariaeAcute and long-term effects of exposure to sodium monofluoroacetate (1080) in sheep author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Article

Indicators

Related links

  • On index processCited by Google
  • On index processSimilars in Google

Share


Onderstepoort Journal of Veterinary Research

On-line version ISSN 2219-0635
Print version ISSN 0030-2465

Abstract

ZWEYGARTH, E.; JOSEMANS, A.I.  and  STEYN, H.C.. In vitro isolation of Ehrlichia ruminantium from ovine blood into Ixodes scapularis (IDE8) cell cultures. Onderstepoort j. vet. res. [online]. 2008, vol.75, n.2, pp.121-126. ISSN 2219-0635.

Four stocks of Ehrlichia ruminantium (Welgevonden, Ball3, Nonile and Blaauwkrans), the causative agent of heartwater in domestic ruminants, were isolated into Ixodes scapularis (IDE8) tick cells using the leukocyte fraction of the blood of infected sheep. Organisms of two of the E. ruminantium stocks (Welgevonden and Blaauwkrans) propagated in IDE8 cells were also successfully used to infect bovine endothelial cells. All stocks were successfully propagated in IDE8 cells using Dulbecco's modified Eagle's medium nutrient mixture Ham F-12 containing 10 % foetal bovine serum (FBS). The technique should be included in any attempt to isolate uncharacterized E. ruminantium stocks.

Keywords : Ehrlichia ruminantium; heartwater; in vitro isolation; tick cell line.

        · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License